Method for determining yield of gellan gum in gellan gum fermentation liquor by utilizing near infrared spectroscopy

A near-infrared spectroscopy and near-infrared spectroscopy technology, which is applied in the direction of measuring devices, material analysis through optical means, instruments, etc., to achieve the effect of saving the use of organic solvents, saving experiment time and production costs

Inactive Publication Date: 2020-04-10
JIANGSU UNIV
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  • Application Information

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Problems solved by technology

However, there is no relevant report on the rapid detection of gellan gum production using near-infrared spectroscopy. The establishment of a method for quickly detecting gellan gum using near-infrared spectroscopy can quickly and accurately detect the production of gellan gum in the fermentation process of gellan gum. It greatly saves the use of organic solvents such as isopropanol or ethanol in traditional methods, which is beneficial to save experimental time and production costs. At the same time, this method can also provide a fast and easy method for the rapid screening of high-yielding gellan gum strains. Gellan gum fermentation to produce high-yield strains and industrial production provides important screening and detection methods

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  • Method for determining yield of gellan gum in gellan gum fermentation liquor by utilizing near infrared spectroscopy
  • Method for determining yield of gellan gum in gellan gum fermentation liquor by utilizing near infrared spectroscopy
  • Method for determining yield of gellan gum in gellan gum fermentation liquor by utilizing near infrared spectroscopy

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Embodiment 1

[0027] Embodiment 1: Sphingomonas paucimobilis strain preservation, seed solution culture and liquid state fermentation method

[0028] 1. Preservation method of Sphingomonas paucimobilis strains: add a ring of slant strains to 50mL liquid medium (beef extract 3g / L, tryptone 5g / L, Nacl 5g / L), 30°C, 200r / L Min cultured for 1 day, the bacterial solution was mixed with 30% (v / v) glycerol at a ratio of 1:1, and placed in a -80°C refrigerator for long-term storage. Streak the bacterial solution in LB solid medium (beef extract 3g / L, tryptone 5g / L, Nacl 5g / L, agar powder 15g / L), place it in an incubator at 30°C for 2 days, and place it in a refrigerator at 4°C. Short term storage.

[0029] 2. Cultivation method of Sphingomonas paucimobilis seed solution: Pick a ring of colony from 4°C LB solid medium and streak it on LB solid medium, culture it in a 30°C incubator for 1 day, and then pick a ring Insert the ring single colony into the seed medium (yeast powder 1g / L, beef extract 3g...

Embodiment 2

[0031] Embodiment 2: Utilize traditional method to analyze the output of gellan gum in the gellan gum fermented liquid

[0032] The specific steps are as follows: start from fermentation 6h, take samples at intervals of 2-4h, accurately weigh 10g (accurate to 0.01g) of fermentation broth in a 250mL Erlenmeyer flask, add 90mL of deionized water, and place the Erlenmeyer flask in a 95°C water bath for 15 -20min, then transferred to a centrifuge tube and centrifuged in a centrifuge at 25°C 8000r / min for 20min, collected the supernatant, and washed the centrifuge tube with an appropriate amount of alcohol, added 250mL industrial alcohol (95%) to the supernatant, placed in overnight in the refrigerator at 4°C. The next day, centrifuge in a centrifuge at 4000r / min for 15 minutes to collect the precipitate, dry it and weigh it to obtain the yield of gellan gum. This invention carried out 6 batches of fermentation, altogether 41 bottles of fermented liquid, each bottle of fermented l...

Embodiment 3

[0035] Embodiment 3: the acquisition of near-infrared spectrum data of gellan gum fermentation liquid

[0036] In order to ensure the accuracy of the modeling effect, the near-infrared spectrum scanning of the fermentation broth was carried out while the gellan gum fermentation broth was collected and tested by traditional methods, and the near-infrared spectrum of the fermentation broth was obtained to obtain the near-infrared spectrum of the gellan gum fermentation broth. Raw data. The near-infrared spectrum collection condition of the present invention is to collect the spectrum under the diffuse reflection mode, and the range of the number of scanning bands of the near-infrared spectrometer is 4000~10000cm -1 . The raw spectrograms of the collected 123 samples are shown in figure 1 .

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Abstract

The invention discloses a method for determining the yield of gellan gum in gellan gum fermentation liquor by utilizing near infrared spectroscopy, belongs to the field of microbial fermentation, andrelates to the technical field of near infrared spectroscopy detection. After the fermentation liquid is sampled at different time points, the yield of gellan gum in the fermentation liquid is analyzed by utilizing a traditional method; meanwhile, a near infrared spectrogram of the fermentation liquid is obtained by scanning of a near infrared spectrograph; an original spectrogram is preprocessedthrough methods such as standard normal variable transformation (SNV), multivariate scattering correction (MSC), smooth filtering (S-G), a normalization method and derivative processing, a mathematical model is established by combining an interval partial least squares method (siPLS), and the model is evaluated through parameters such as correlation coefficients and standard root mean square errors. The method is simple and quick to operate and pollution-free, can greatly save the experimental time and cost in the screening of high-yield gellan gum strains, and can also be used for laboratoryor industrial production of gellan gum.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and at the same time relates to the technical field of near-infrared spectrum detection, and specifically provides a method for quickly measuring the yield of gellan gum in gellan gum fermentation liquid by using near-infrared spectroscopy Background technique [0002] Gellan gum is a microbial polysaccharide successfully developed by the American Kelco company after xanthan gum. It is produced by aerobic fermentation of Sphingomonas paucimobilis. It has good stability and is acid-resistant, With the advantages of high temperature resistance, thermal reversibility, and less dosage, the gel strength of 1.5% agar or 1% carrageenan can be achieved by using 0.25% gellan gum, which is gradually replacing agar and carrageenan in industrial applications . However, due to the relatively low yield of gellan gum, and the cumbersome steps of traditional chemical methods to detect gellan gum, it is imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 孙玲王亚珍孙文敬刘利平任晓锋何荣海马海乐
Owner JIANGSU UNIV
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