Lignocellulose-degrading composite microbial system and culture method and application thereof

A technology of lignocellulose and composite bacteria, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve problems such as low degradation speed and single microorganisms

Inactive Publication Date: 2020-03-31
陕西山河生物科技有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problem that the existing lignocellulose biodegradation process is mainly aimed at a single microorganism and has a low degradation rate, the present invention uses rotten straw as a new biological resource to provide a lignocellulose-degrading composite strain and its cultivation Methods and Applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lignocellulose-degrading composite microbial system and culture method and application thereof
  • Lignocellulose-degrading composite microbial system and culture method and application thereof
  • Lignocellulose-degrading composite microbial system and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] This embodiment mainly involves the screening of highly efficient biomass cellulose degrading bacteria, and the construction of a composite bacterial system, mainly including:

[0132] 1. Test plan

[0133] 1.1 Sample processing and preliminary screening

[0134] 1.1.1, sample processing

[0135] Accurately weigh 10.0 g of the straw sample, add it into a 250 mL Erlenmeyer flask containing 90 mL of sterile saline and glass beads, shake it on a constant temperature shaker at 37 ° C, and mix the sample thoroughly for 3 hours for later use.

[0136] 1.1.2. Primary screening

[0137] a) Dilute the collected samples step by step 10 times with sterile water to 10 -6 、10 -7 and 10 -8 After doubling, take 0.1mL and spread it on the cellulose Congo red selective medium, repeat the coating on 3 plates for each concentration, culture at 28°C, observe regularly, and pick the strains with clear transparent circles around the colonies;

[0138] b) Purify the picked strains by st...

Embodiment 2

[0209] In this example, the complex bacterial strain constructed by the high-efficiency biomass cellulose degrading bacteria screened out in Example 1 is used to prepare the complex bacterial enzyme preparation, and the process flow is as follows Figure 11 As shown, it mainly includes the process of strain cultivation, fermentation process, extraction, separation and purification of enzymes, finished enzymes, testing and packaging.

[0210] Process operation points

[0211] 1. Bacteria culture

[0212] The compound strains of selected fungi, bacteria and actinomycetes are amplified and cultured on the corresponding medium. Specific steps:

[0213] 1.1) Clean the inclined surface with sterile physiological saline, shake well, put it into a sterile flask and shake well;

[0214] 1.2) Inoculate the oblique culture in the seed bottle, cultivate the bacteria at 30°C and 180r / rain for 12 hours to obtain the seed liquid, and cultivate the mold at 28°C and 220r / rain for 48 hours t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
densityaaaaaaaaaa
Login to view more

Abstract

The invention provides a lignocellulose-degrading composite microbial system and a culture method and an application thereof, and solves problems that an existing lignocellulose biodegradation processmainly aims at a single microbe and has a low degradation speed. The composite microbial system includes trichoderma, penicillium, clostridium, bacillus and herbaspirillum, wherein the trichoderma, the penicillium, the clostridium, the bacillus and the herbaspirillum respectively have a mass percent of 20%-40%, 20%-40%, 5%-10%, 20%-40% and 5%-10%; a composite microbial culture medium for culturing the composite microbial system is a cellulose congo red culture medium; and a fermentation culture medium using the composite microbial system includes the following components: 5% of wheat bran, 20% of maize straw powder, 0.05% of yeast extract, 0.3% of peptone, 0.03% of magnesium sulfate heptahydrate, 0.4% of potassium dihydrogen phosphate, 0.03% of calcium chloride dihydrate, 1.5% of ammoniumsulfate, 0.8% of tween 80, and the balance being water. The composite microbial system has an application in straw degradation.

Description

technical field [0001] The invention relates to the compound bacterial agent technology, in particular to a lignocellulose-degrading compound bacterial strain and its cultivation method and application. Background technique [0002] In recent years, the global food crisis, energy crisis, and environmental crisis have become increasingly serious. People have gradually turned their attention to the most abundant and cheapest lignocellulose resources on the earth. Lignocellulose is a mixture of cellulose, hemicellulose, and lignin. , is the largest renewable resource on earth. Microbial degradation and conversion of lignocellulose is an important part of the natural carbon cycle. If this resource is effectively utilized, it will have far-reaching significance in solving the energy shortage and environmental pollution problems facing the world today. [0003] Since about 80% of corn stover components are cellulose, hemicellulose, and lignin, and lignin that is difficult to degr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/22C12N9/42C12Q1/04C12Q1/689C12Q1/6895C12Q1/54C12Q1/34C12R1/885C12R1/80C12R1/145C12R1/07C12R1/01
CPCC12N1/14C12N1/20C12N1/22C12N9/2437C12Q1/045C12Q1/34C12Q1/54C12Q1/689C12Q1/6895G01N2333/32G01N2333/33G01N2333/385G01N2333/942
Inventor 刘国庆钱晓勇赵肖马翔
Owner 陕西山河生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap