Method for simultaneously detecting multiple anti-tumor drugs in blood sample
A technology for anti-tumor drugs and blood samples, applied in the field of blood drug concentration detection, can solve problems such as indistinguishability, inability to predict curative effects, assess adverse reactions, and drug concentration deviations, and achieve reliable results and high throughput. , to ensure specific effects
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[0076] (1) Prepare the sample to be tested
[0077] Add methanol:acetonitrile (volume ratio 1:1) solution to the serum sample and quality control sample, vortex and centrifuge, take the supernatant into a sample bottle, blow dry with nitrogen, and redissolve the sample in 70% v / v In methanol aqueous solution, filter through a 0.2 μm filter membrane to obtain the sample to be tested.
[0078] Wherein, the volume ratio of serum, methanol, and acetonitrile is 1:1:1, and the volume ratio of serum to 70% methanol aqueous solution is 1:1.
[0079] (2) Ultra-high performance liquid chromatography-mass spectrometry detection
[0080] The sample to be tested enters the chromatographic column for separation through the gradient elution mode, and the reference conditions of liquid chromatography are:
[0081] Chromatographic column: C18 column (Waters ACQUITY HPLC BEH C18 100mm×2.1mm, 1.7μm)
[0082] Column temperature: 40°C
[0083] Injection volume: 20μl
[0084] Flow rate: 250μl...
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