Application of gallic acid in agaricus gennadii in CDC25 phosphoprotease

A phosphoprotease and gallic acid technology, which is applied in the directions of medical preparations containing active ingredients, preparation of carboxylate esters, preparation of organic compounds, etc. The effect of simple separation and low production cost

Inactive Publication Date: 2020-02-28
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical constituents and related activities of Agaricus gennadii in the same genus are still in the blank

Method used

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  • Application of gallic acid in agaricus gennadii in CDC25 phosphoprotease
  • Application of gallic acid in agaricus gennadii in CDC25 phosphoprotease
  • Application of gallic acid in agaricus gennadii in CDC25 phosphoprotease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of the crude extract of each active component in the mushroom tortuosporum of embodiment 1

[0036] Take 10kg of fresh, undamaged Agaricus torusum fruiting body, slice it and soak it with 30L 95% ethanol for 7 days (wherein the ratio of Agaricus toricosporin to ethanol is 1:3, so that the ethanol completely soaks the fruiting body of Agaricus torisporum), filter The filtrate was obtained, the solvent was recovered under reduced pressure, and 30 L of 95% ethanol was added to the filter residue for ultrasonic extraction 3 times, each time for 2 hours. Concentrate under reduced pressure to obtain 95% ethanol extract. Operate in the same way with 65% ethanol, and concentrate under reduced pressure to obtain 65% ethanol extract. Add 30L of distilled water to the filter residue of the mushroom after alcohol extraction, and extract three times with 1800HZ ultrasound, each time for 2 hours. Concentrate under reduced pressure to obtain 538 g of water extract.

[0...

Embodiment 2

[0041] Example 2 Petroleum ether phase component separation and purification

[0042] 10.0 g of the petroleum ether fraction obtained in the above example 1 was separated by medium-pressure silica gel column flash chromatography, petroleum ether-ethyl acetate system (100:0-0:100) gradient elution, and the eluent was detected by thin-layer chromatography. The similar fractions were combined, and the solvent was recovered to obtain a total of 9 components A to I. Component D is separated by medium-pressure silica gel chromatography to obtain D1 and D2. D1 was recrystallized to obtain 20 mg of compound 3 in the form of needles. D2 was separated by multiple medium-pressure silica gel chromatography and Sephadex LH-20 column chromatography to obtain 10 mg of compound 2 as a white solid. Component E was separated by multiple medium-pressure silica gel chromatography and Sephadex LH-20 column chromatography to obtain 5 mg of compound 1 as a yellow-green oily solid.

[0043] Among ...

Embodiment 3

[0055] Example 3 Separation and Purification of Ethyl Acetate Part Components

[0056] 6.0 g of the ethyl acetate fraction obtained in the above-mentioned Example 1 was reversed phase C by medium pressure 18 Column flash chromatography separation, water-methanol solvent system (5% to 100%) gradient elution, the eluents are combined according to the same gradient, and the solvent is recovered to obtain 6 components from I to VI. After checking by high performance liquid chromatography, it was found that components I to IV were common components in the n-butanol phase, so they were merged into the n-butanol phase. Ⅴ component through medium pressure reverse phase C 18 After detaching, use C again 18 After semi-preparative column separation, a total of 4 mg of compound 4, a total of 4 mg of compound 5 and a total of 3 mg of compound 24 were obtained. Compound 4 is a yellow granular solid; Compound 24 is a yellow granular solid; Compound 5 is a red granular solid.

[0057] whe...

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Abstract

The invention discloses application of gallic acid which is an active ingredient in agaricus gennadii. Active ingredients of agaricus gennadii are quickly separated through a separation and extractionmethod, and a separation and extraction process is simple, stable, suitable for industrial continuous production, high in product yield and low in production cost; the gallic acid in the active ingredients of agaricus gennadii has effect of inhibiting activity of CDC25 phosphoprotease, thereby being supportive of being applied to diseases related to CDC25 phosphoprotease.

Description

Technical field: [0001] The invention relates to the application technical field of natural products, an application of gallic acid which is an active ingredient extracted from natural products, and more specifically relates to the extraction of gallic acid from mushroom tortuosporum and the application of gallic acid on CDC25 phosphoprotease. Background technique: [0002] Mushroom family (Agaricaceae) is a family of fungi Agaricaceae, with many types and wide distribution. Most of them are edible, and they are distributed all over the world. The mushroom family has 28 genera including the genus Agaricus. Agaricus gennadii (Chot.et Boud) P.D.Orton, Agaricus bisporus (Large) Sing., Agaricus blazei murrill, Agaricus rubellus (Gill.) Sacc., brown mushroom Agaricus crocopelusspeck, etc. At present, researches on the chemical constituents and pharmacological activities of this genus are mainly focused on Agaricus bisporus and Agaricus blazei. [0003] The research on the chem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/235A61K31/575A61K31/498A61K31/192A61K31/122A61K31/58A61K31/357A61K31/7076A61K31/11A61K31/085A61K31/05A61K31/075A61P35/00C07C51/47C07C51/48C07C65/03C07C67/56C07C67/58C07C69/82C07J9/00C07D241/46C07C65/36C07C46/10C07C50/34C07J71/00C07D323/00C07H1/08C07H19/167C07C69/84C07C45/79C07C45/80C07C47/565C07C41/36C07C41/38C07C43/23C07C37/82C07C37/72C07C39/11C07C43/178
CPCA61K31/235A61K31/575A61K31/498A61K31/192A61K31/122A61K31/58A61K31/357A61K31/7076A61K31/11A61K31/085A61K31/05A61K31/075A61P35/00C07C51/47C07C51/48C07C67/56C07C67/58C07J9/00C07D241/46C07C46/10C07J71/0005C07D323/00C07H1/08C07H19/167C07C45/79C07C45/80C07C41/36C07C41/38C07J71/001C07C37/82C07C37/72C07C2602/30C07C2603/24C07C65/03C07C65/36C07C69/82C07C69/84C07C50/34C07C47/565C07C43/23C07C43/1782C07C39/11
Inventor 林鹏程廖志明吴疆
Owner QINGHAI UNIV FOR NATITIES
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