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Lumbrukinase concentrated slurry capable of realizing normal-temperature environment storage and long-distance transportation

A technology of long-distance transportation and production process, applied in the field of lumbrokinase, can solve the problems of high cost and long time, and achieve the effects of stable physical and chemical properties, reducing load, and improving the abundance of lumbrokinase

Inactive Publication Date: 2020-02-25
倪成 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the freeze-drying storage method can avoid the problem of enzyme inactivation caused by high temperature, it is costly and time-consuming, and sales and applications need to be carried out at low temperatures

Method used

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  • Lumbrukinase concentrated slurry capable of realizing normal-temperature environment storage and long-distance transportation
  • Lumbrukinase concentrated slurry capable of realizing normal-temperature environment storage and long-distance transportation
  • Lumbrukinase concentrated slurry capable of realizing normal-temperature environment storage and long-distance transportation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] a) Collect earthworm slurry

[0057] After the sundries in the abdominal cavity of the earthworms are emptied and cleaned, they are ground into a slurry with a grinding wheel mill to obtain an earthworm slurry; the temperature of the earthworm slurry preparation process is 15°C, and the time is 8 hours;

[0058] b) Continuous centrifugal deslagging

[0059] The earthworm grinding slurry obtained in step a) is centrifuged to remove slag at 12000r / min to obtain a supernatant;

[0060] c) clarification filter

[0061] To the supernatant obtained in step b), add a coiled membrane module with a molecular weight cut-off of 50KD to clarify the feed liquid;

[0062] d) Degreasing treatment

[0063] Add sodium chloride to the feed liquid obtained in step c) until the concentration of sodium chloride reaches 1M, centrifuge at 10000r / min for 30min, and the earthworm grease phase is separated;

[0064] e) Desalination and concentration

[0065] Pass the clarified liquid obtain...

Embodiment 2

[0067] The difference between embodiment 2 and embodiment 1 is only that the whole operation process of step a) to step e) is operated under the continuous irradiation of ultraviolet rays emitted by an ultraviolet device with a power of 300W, and the others are exactly the same as embodiment 1.

Embodiment 3

[0069] a) Collect earthworm slurry

[0070] After the sundries in the earthworm abdominal cavity are emptied and cleaned, the ball mill is used to grind to make a slurry to obtain the earthworm slurry; the temperature of the earthworm slurry preparation process is 10°C, and the time is 6 hours;

[0071] b) Continuous centrifugal deslagging

[0072] The earthworm grinding slurry obtained in step a) is centrifuged to remove slag at 12000r / min to obtain a supernatant;

[0073] c) clarification filter

[0074] Adding a sintered metal membrane module with a molecular weight cut-off of 50KD to the supernatant obtained in step b) to clarify the feed liquid;

[0075] d) Degreasing treatment

[0076] Add sodium chloride to the feed liquid obtained in step c) until the concentration of sodium chloride reaches 1M, centrifuge at 10000r / min for 30min, and the earthworm grease phase is separated;

[0077] e) Desalination and concentration

[0078] Pass the clarified liquid obtained in ...

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Abstract

The invention discloses a lumbrukinase concentrated slurry production process capable of realizing normal-temperature environment storage and long-distance transportation. The method comprises the steps of pulping, deslagging, filtering, degreasing, desalting and concentrating, sterilizing and the like, impurities such as water-soluble impurities, denatured protein, earthworm grease and the like can be removed, the abundance of lumbrukinase in the lumbrukinase concentrated slurry is improved, the load of a separation medium of a production line is reduced, and the yield of lumbrukinase is increased; moreover, the produced lumbrukinase concentrated slurry is stable in physicochemical property, and the enzyme activity of the lumbrukinase concentrated slurry is maintained for a long time. Theproduction process is suitable for different lumbrukinase process routes, and the prepared lumbrukinase concentrated slurry can be directly used as an earthworm body raw material substitute of lumbrukinase refining engineering, can be stored and transported at normal temperature, has the characteristic of perennial quality guarantee, and is suitable for initial raw materials produced by lumbrukinase factories.

Description

technical field [0001] The invention relates to the field of lumbrokinase, in particular to a production process of lumbrokinase concentrated slurry that can be stored in a normal temperature environment and transported over long distances. Background technique [0002] Lumbrokinase is a group of proteolytic enzymes (acidic proteins) extracted from special earthworms, with a molecular weight of 16,000-45,000 D. Lumbrokinase is an antithrombotic drug, and it is mainly used clinically for thrombotic diseases, especially for patients with increased fibrinogen and increased platelet aggregation rate; it is used for ischemic cardiovascular and cerebrovascular diseases, which can improve symptoms and prevent disease progression. [0003] However, due to the influence of external environmental factors during the processing and storage of lumbrokinase, when the environment changes or the interaction of some groups is weakened, it is easy to cause the collapse of the enzyme structure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/68C12N9/64
CPCC12N9/6405C12N9/6408C12Y304/21007
Inventor 倪成倪宝谦
Owner 倪成
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