Extraction process for optimizing total flavonoids of potamogeton distinctus by response surface method

An extraction process and a technology of response surface method, which is applied in the field of extraction process optimization of total flavonoids of vegetal seedlings by response surface method, can solve the problems such as not seeing the extraction process of vegetal seedlings, and achieve scientific extraction, high extraction efficiency, Ease of promoting the effect of the application

Pending Publication Date: 2020-02-21
VOCATIONAL & TECHN COLLEGE OF ANSHUN
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  • Extraction process for optimizing total flavonoids of potamogeton distinctus by response surface method
  • Extraction process for optimizing total flavonoids of potamogeton distinctus by response surface method
  • Extraction process for optimizing total flavonoids of potamogeton distinctus by response surface method

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Embodiment Construction

[0038] The principles and features of the present invention are described below in conjunction with the examples, which are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0039] A response surface method to optimize the extraction process of total flavonoids of S. chinensis, comprising the following steps:

[0040] (1) Preparation of reference substance solution: Weigh 50.9 mg of rutin reference substance dried at 120 °C to constant weight, put it in a beaker, add ethanol solution, dissolve in a water bath at 45 °C-55 °C, and dilute to 50 mL In the volumetric flask, shake well to obtain the rutin control solution of 1.018mg / mL;

[0041] (2) Preparation of sample solution: Grind the spinach, pass through a 20-40 mesh sieve, weigh 1 g of coarse powder, place it in a conical flask with a stopper, add ethanol solution for ultrasonic extraction, cool to room temperature, and filter , add ethanol solution to make up f...

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Abstract

The invention discloses an extraction process for optimizing total flavonoids of potamogeton distinctus by a response surface method. The process comprises the following steps of (1) preparing a control solution; (2) preparing a sample solution; (3) detecting the wavelength; (4) making a standard curve; (5) establishing and analyzing a regression model; and (6) analyzing and optimizing an experimental result. The extraction process is simple, high in extraction efficiency and easy to popularize and apply; the response surface method is used for optimization to determine optimal factors; the scientificity and stability of extraction are guaranteed; and a basis is provided for quality standard research.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a response surface method to optimize the extraction process of total flavonoids of vegetal plants. Background technique [0002] Potamogeton distinctus (Potamogeton distinctus A. Bennett J. Bot.) is the whole herb of the perennial aquatic herbaceous plant of Potamogeton distinctus A. Bennett J. Bot. Commonly known as floating on water, also known as water chopping board, it is distributed in all rice-growing areas of the country. It is a perennial vicious weed in rice fields. It mainly relies on chicken feet buds (bulbs) for asexual reproduction and causes harm all year round. In the fourth national census of traditional Chinese medicine resources, it was found that fenugreek is widely distributed in Guizhou, and the folks in Anshun area (Tunpu people) have better effects in treating hemorrhoids and hepatitis. In Ming Dynasty Lanmao's "Southern Yunnan Mater...

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 洪开文宋伦郑重
Owner VOCATIONAL & TECHN COLLEGE OF ANSHUN
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