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Preparation for direct writing forming 3D printing bioink, and 3D printing method capable of adopting direct writing forming 3D printing bioink

A 3D printing and bio-ink technology, applied in 3D object support structures, additive manufacturing, manufacturing tools, etc., can solve the problems of easy fiber dispersion and poor stability, achieve high biocompatibility, adjustable viscosity, and improve survival rate Effect

Active Publication Date: 2020-02-14
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cellulose nanofibers can self-cure to maintain their original shape and structure, cellulose nanofibers are easily dispersed in water and have poor stability. Therefore, a non-toxic and simple curing method is needed to increase its stability.

Method used

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  • Preparation for direct writing forming 3D printing bioink, and 3D printing method capable of adopting direct writing forming 3D printing bioink
  • Preparation for direct writing forming 3D printing bioink, and 3D printing method capable of adopting direct writing forming 3D printing bioink
  • Preparation for direct writing forming 3D printing bioink, and 3D printing method capable of adopting direct writing forming 3D printing bioink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for preparing direct writing 3D printing bio-ink includes the following steps:

[0043] S1, Preparation of cellulose nanofiber methacrylate freeze-dried fiber

[0044] Disperse 1 to 5 g of cellulose nanofiber powder in deionized water at 4 to 50° C., rotate at a speed of 200 to 1000 r / min, and mechanically stir for 30 to 120 minutes to achieve uniform dispersion. Stir for 12-24 hours in a water bath at 4-25°C to obtain a cellulose nanofiber dispersion with a mass percentage of 1 to 5%. According to the quality of the cellulose nanofiber powder, add 1-20 times the volume of methacrylic anhydride liquid in the dispersion liquid at a rate of 0.5-1mL / min. After reacting for 2-3h, add 3-10mol / L of hydroxide With sodium solution, adjust the pH value of the mixed solution to the range of 8.0-9.0, and continue to react for 12-24 hours to obtain the cellulose nanofiber methacrylate precursor. The cellulose nanofiber methacrylate precursor is clamped with a 5000-14000 molec...

Embodiment 2

[0061] A direct-write molding 3D printing bio-ink. The preparation process is the same as that of Example 1, except that in step S1, 1g of cellulose nanofiber powder is added, and the prepared cellulose nanofiber methacrylate solution contains fiber The mass percentage of the plain nanofiber methacrylate is 1%, the mass percentage of the photoinitiator is 0.05%, the mass percentage of the PBS buffer is 98.95%, and the sum of the mass percentages of the above components is 100%.

[0062] The LAP photoinitiator used in the present invention has an improved polymerization reaction kinetic mechanism, so that the polymer hydrogel or other polymer materials can encapsulate biological cells under lower initiator concentration and longer wavelength light. Under the action of blue light, LAP quickly initiates the curing of the photosensitive hydrogel material. Compared with ultraviolet light initiators, blue light does less damage to biological cells, thereby increasing the survival rate ...

Embodiment 3

[0064] A direct-write molding 3D printing bio-ink. The preparation process is the same as that in Example 1, except that in step S1, 5g of cellulose nanofiber powder is added, and the prepared cellulose nanofiber methacrylate solution contains fiber The mass percentage of the plain nanofiber methacrylate is 5%, the mass percentage of the photoinitiator is 0.5%, the mass percentage of the PBS buffer is 94.5%, and the sum of the above-mentioned components is 100%.

[0065] The cellulose nanofiber used in the invention is a natural polymer material, which has the characteristics of adjustable viscosity, structural orientation, wide sources, and good mechanical properties. Grafting olefin groups into the cellulose nanofiber system makes it obtain the photocurable properties of synthetic polymers. The blue light initiates photocuring to prepare stable cellulose nanofiber methacrylate hydrogel.

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Abstract

The invention discloses preparation for a direct writing forming 3D printing bioink, and a 3D printing method capable of adopting the direct writing forming 3D printing bioink. The 3D printing bioinktakes a cellulose nanofiber as a main body, and methacrylic anhydride is grafted to a main body chain segment to synthesize cellulose nanofiber methacrylate. By use of the shear thinning characteristic of a cellulose nanofiber hydrogel and a characteristic that a methacrylate polymer is photocurable, the degradable 3D printing bioink which has high biocompatibility, high mechanical properties, notoxins and structure orientations and has a capability of guiding biological cells to grow and differentiate is prepared. A biological bionic structure printed by the 3D printing bioink is suitable for biological cells to adhere, proliferate and differentiate in the biological bionic structure, and the biological bionic structure is an ideal biological tissue reconstruction and repairing substitute.

Description

Technical field [0001] The invention relates to the field of 3D printing biological ink preparation, in particular to a direct-writing molding 3D printing biological ink preparation and a 3D printing method thereof. Background technique [0002] Direct ink writing (DIW) is a branch of 3D printing technology. The molding process of direct writing 3D printing technology is different from the traditional mechanical molding method that requires a mold to form, and it is a free molding method with a wide range of uses. [0003] Direct writing 3D printing technology is a technology that precisely forms materials with specific components according to the structure set by computer software. The ink material installed in the Z axis direction is moved and extruded on the XY printing platform to form the required three-dimensional structure. . Among them, the ink material is generally stored in a temperature-controllable barrel. The nozzle is connected to the barrel and fixed on the Z-axis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/38A61L27/52A61L27/60B29C64/393B29C64/264B33Y30/00B33Y50/02
CPCA61L27/20A61L27/3821A61L27/3826A61L27/52A61L27/60A61L2430/30B33Y30/00B33Y50/02B29C64/264B29C64/393C08B3/08C08L1/02
Inventor 吴耀彬黄文华许益超利时雨
Owner SOUTHERN MEDICAL UNIVERSITY
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