Chitosan gelatin having physiological response properties and application of chitosan gelatin to prevention adhesion in uterine cavity

A chitosan gel, responsive technology, applied in the field of pharmacy, can solve problems such as inflammatory reaction, not meeting safety requirements, etc., to avoid injury, promote rapid healing, and facilitate industrialization implementation.

Inactive Publication Date: 2020-02-07
XI AN JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, poloxamer and chitosan are the main temperature-sensitive gel materials in the field of life sciences, but chitosan as the main matrix needs to be shared with glycerophosphate to achieve temperature-sensitive properties, and glycerophosphate has certain toxicity and is easily absorbed in the body. Inflammation occurs, does not meet safety requirements

Method used

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  • Chitosan gelatin having physiological response properties and application of chitosan gelatin to prevention adhesion in uterine cavity
  • Chitosan gelatin having physiological response properties and application of chitosan gelatin to prevention adhesion in uterine cavity
  • Chitosan gelatin having physiological response properties and application of chitosan gelatin to prevention adhesion in uterine cavity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of chitosan gel with physiological responsiveness for preventing intrauterine adhesions 1.1 Screening of prescription

[0043] The range of P407 mass concentration (w / v) is locked at 17%-21%, the range of P188 mass concentration (w / v) is locked at 1%-3%, with P407 and P188 as independent variables, gelation temperature as effect size, Establish star point design, see Table 1:

[0044] Table 1. Star point design-response surface optimization method factor level table

[0045]

[0046] Preparation method: Add the weighed calculated amount of P407 and P188 into the container, add the calculated amount of purified water, and stir until the solid swells infinitely. Add 0.5% chitosan hydrochloride and stir until completely dissolved.

[0047] Result analysis: The coding factor equation of this model is obtained by design expert:

[0048] T=26.5-6.79*A+2.59*B-0.85*A*B+2.07A 2 +1.04B 2

[0049] F=93.00, P<0.0001<0.01, indicating that this regressi...

Embodiment 2

[0074] Example 2 Intervention of chitosan gel with physiological responsiveness on rat uterine adhesion model

[0075] 2.1 Modeling method

[0076] Rats in estrus were injected intraperitoneally with 10% chloral hydrate solution 30ml / kg, and after anesthesia, they were fixed in supine position on an operating board with a constant temperature pad. Remove hair from the abdomen of rats, disinfect the abdomen of rats with iodine, open a wound of about 3 cm along the lower part of the midline of the abdomen, expose the uterus, make an incision of 2 mm in the upper part of the middle of the uterus, and gently scrape the distal end with a curettage spoon (length About 3cm), until there is a rough feeling and then gradually smooth (the uterus can be supported by the fingertips), and the uterus is sutured. Clamp the uterine artery, ovarian artery-uterine artery communication branch, bilateral proximal uterus, and bilateral distal uterus with atraumatic vascular clamp, puncture from t...

Embodiment 3

[0106] Embodiment 3 has the interventional effect of chitosan gel with physiological responsiveness to mouse abdominal cavity model

[0107] 3.1 Modeling

[0108] The mice were weighed and anesthetized by intraperitoneal injection of 3.5% chloral hydrate at 0.01 ml / g. Electric hair remover to shave belly hair. The whole body was sprayed with 75% ethanol for disinfection, and the abdomen was disinfected with povidone iodine. The mouse was fixed on the foam board in the supine position, and the laparotomy was opened in layers with surgical scissors. Two hemostatic forceps clamp the muscle layer on the right side and turn it over so that the abdominal mucosa faces outward. Use a small blade to gently draw a rectangular area of ​​1*0.5cm, and then use tweezers to remove the abdominal mucosa in the area, as shown in Figure 7 As shown, remove the hemostat. Take out the cecum, and use gauze to wear around the cecum about 20 times until there is bleeding, such as Figure 7 As sh...

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Abstract

The invention discloses chitosan gelatin having physiological response properties and an application of the chitosan gelatin to prevention adhesion in uterine cavity, and belongs to the technical field of pharmaceutics. The chitosan gelatin is prepared through the steps of adding active components having functions of stopping bleeding, resisting bacteria, promoting healing and the like to a non-chemical crosslinking thermo-sensitive bacteria-free water gelatin substrate, wherein under the room-temperature condition, the gelatin being favorable in flowing properties and being in the state of sol is convenient for medication in the uterine cavity, at the physiology temperature of wombs, the gelatin rapidly gelate and is solidified, physical isolation on wound parts after an intrauterine operation is completed, adhesion is avoided, besides, the functions of a biologicalactivity material are exerted, and quick healing of wound parts is promoted. After the wombs are restored, under the physiological action of proliferation and secretion, corrosion and erosion of the gelatin are completed, the gelatin is discharged outside the bodies along with secretion, and human intervention is not needed. The gelatin has the advantages of being simple to apply, capable of predicting effects, free from secondary intervene and the like, and can be used for preventing adhesion after intrauterine operations.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and relates to a chitosan gel with physiological responsiveness and its application in uterine cavity anti-adhesion. Background technique [0002] At present, after uterine surgery, estrogen is often used clinically to promote endometrial repair and prevent Intrauterine Adhesion (IUA), but there is basically no other active intervention. Some reports show that after gynecological surgery, different The incidence of postoperative IUA is as high as 50%. The clinical manifestations of IUA are mainly decreased menstrual flow or even amenorrhea, dysmenorrhea, secondary infertility, and adverse pregnancy outcomes, including recurrent miscarriage, premature delivery, and placenta previa. [0003] Hysteroscopic separation of intrauterine adhesions (Trans Cervical Resection of Adhesion, TCRA) is currently the main means of treatment of IUA. In order to prevent the recurrence of IUA after TCRA treatment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/06A61L31/14
CPCA61L31/042A61L31/06A61L31/14A61L31/145A61L2400/04C08L5/08C08L71/02
Inventor 张继业杨显伟李建莫菲马佳张沛沛
Owner XI AN JIAOTONG UNIV
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