Biological repair membrane and preparation method thereof
A bioremediation, animal membrane technology, applied in medical science, prosthesis, coating, etc., can solve problems such as weakening the performance of promoting tissue regeneration and repairing, adhesion and inducing other complications, cerebrospinal fluid leakage at the suture point, etc. Beneficial for healing, excellent mechanical strength and tight bonding
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Embodiment 1
[0087] The bioprosthetic membrane for dura / spinal repair is made of bovine-derived biomaterials through the following steps;
[0088] Step 1. Animal membrane tissue pretreatment: The selected bovine pericardium refers to the selection of qualified slaughterhouses and the selection of healthy cattle. Tear off the easy-to-tear fat attached to the bovine pericardium, then spread the bovine pericardium on a stainless steel plate, scrape off the remaining fat with a card, and cut off the parts that are difficult to remove fat or impurities on the edge; add purified The bovine pericardium was washed repeatedly with water until the surface was free of blood and other attachments.
[0089] Step 2. Degreasing: add isopropanol in a fume hood at a material-to-liquid ratio of 1:8 (weight-to-volume ratio), degrease by shaking at 120 rpm at room temperature for 4 hours, replace the isopropanol, and continue shaking and degreasing at 120 rpm at room temperature for 11 hours; Discard the iso...
Embodiment 2
[0100] The bioprosthetic membrane for dura / spinal repair is made of bovine-derived biomaterials through the following steps;
[0101] Step 1. Animal membrane tissue pretreatment: The selected bovine pericardium refers to the selection of qualified slaughterhouses and the selection of healthy cattle. Tear off the easy-to-tear fat attached to the bovine pericardium, then spread the bovine pericardium on a stainless steel plate, scrape off the remaining fat with a card, and cut off the parts that are difficult to remove fat or impurities on the edge; add purified The bovine pericardium was washed repeatedly with water until the surface was free of blood and other attachments.
[0102] Step 2. Degreasing: add isopropanol in a fume hood at a material-to-liquid ratio of 1:8 (weight-to-volume ratio), degrease by shaking at 120 rpm at room temperature for 4 hours, replace the isopropanol, and continue shaking and degreasing at 120 rpm at room temperature for 11 hours; Discard the iso...
Embodiment 3
[0110] The bioprosthetic membrane for dura / spinal repair is made of pig-derived biomaterials through the following steps;
[0111] Step 1. Animal membrane tissue pretreatment: The selected pig peritoneum refers to the selection of qualified slaughterhouses and the selection of healthy pigs. Tear off the easy-to-tear fat attached to the pig's peritoneum, then spread the pig's peritoneum on a stainless steel plate, scrape off the remaining fat with a card, and cut off the parts that are difficult to remove fat or impurities on the edge; add purified water to wash the pig repeatedly Peritoneum, to the surface without blood and other attachments.
[0112] Step 2. Degreasing: add isopropanol in a fume hood at a material-to-liquid ratio of 1:8 (weight-to-volume ratio), degrease by shaking at 120 rpm at room temperature for 4 hours, replace the isopropanol, and continue shaking and degreasing at 120 rpm at room temperature for 11 hours; Discard the isopropanol, wash the isopropanol ...
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