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Biological repair membrane and preparation method thereof

A bioremediation, animal membrane technology, applied in medical science, prosthesis, coating, etc., can solve problems such as weakening the performance of promoting tissue regeneration and repairing, adhesion and inducing other complications, cerebrospinal fluid leakage at the suture point, etc. Beneficial for healing, excellent mechanical strength and tight bonding

Active Publication Date: 2017-01-11
SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the existing problems of poor mechanical strength, risk of cerebrospinal fluid leakage at the suture point, weakened ability to promote tissue regeneration and repair, residual toxicity after degradation, and poor biocompatibility in the existing biorepair membrane, which may easily cause the repair material to be incompatible with brain tissue. Adhesion and induce other defects such as complications, and provide a leak-proof, hemostatic, anti-adhesion performance, without sutures can seal the wound and maintain good mechanical properties, good biocompatibility, no immune rejection , no adverse stimulation to the tissue; degradable and absorbable, the degradation rate matches the repair rate of the dura / spinal mater, a biological repair membrane suitable for dura / spinal repair and its preparation method

Method used

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  • Biological repair membrane and preparation method thereof
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  • Biological repair membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] The bioprosthetic membrane for dura / spinal repair is made of bovine-derived biomaterials through the following steps;

[0088] Step 1. Animal membrane tissue pretreatment: The selected bovine pericardium refers to the selection of qualified slaughterhouses and the selection of healthy cattle. Tear off the easy-to-tear fat attached to the bovine pericardium, then spread the bovine pericardium on a stainless steel plate, scrape off the remaining fat with a card, and cut off the parts that are difficult to remove fat or impurities on the edge; add purified The bovine pericardium was washed repeatedly with water until the surface was free of blood and other attachments.

[0089] Step 2. Degreasing: add isopropanol in a fume hood at a material-to-liquid ratio of 1:8 (weight-to-volume ratio), degrease by shaking at 120 rpm at room temperature for 4 hours, replace the isopropanol, and continue shaking and degreasing at 120 rpm at room temperature for 11 hours; Discard the iso...

Embodiment 2

[0100] The bioprosthetic membrane for dura / spinal repair is made of bovine-derived biomaterials through the following steps;

[0101] Step 1. Animal membrane tissue pretreatment: The selected bovine pericardium refers to the selection of qualified slaughterhouses and the selection of healthy cattle. Tear off the easy-to-tear fat attached to the bovine pericardium, then spread the bovine pericardium on a stainless steel plate, scrape off the remaining fat with a card, and cut off the parts that are difficult to remove fat or impurities on the edge; add purified The bovine pericardium was washed repeatedly with water until the surface was free of blood and other attachments.

[0102] Step 2. Degreasing: add isopropanol in a fume hood at a material-to-liquid ratio of 1:8 (weight-to-volume ratio), degrease by shaking at 120 rpm at room temperature for 4 hours, replace the isopropanol, and continue shaking and degreasing at 120 rpm at room temperature for 11 hours; Discard the iso...

Embodiment 3

[0110] The bioprosthetic membrane for dura / spinal repair is made of pig-derived biomaterials through the following steps;

[0111] Step 1. Animal membrane tissue pretreatment: The selected pig peritoneum refers to the selection of qualified slaughterhouses and the selection of healthy pigs. Tear off the easy-to-tear fat attached to the pig's peritoneum, then spread the pig's peritoneum on a stainless steel plate, scrape off the remaining fat with a card, and cut off the parts that are difficult to remove fat or impurities on the edge; add purified water to wash the pig repeatedly Peritoneum, to the surface without blood and other attachments.

[0112] Step 2. Degreasing: add isopropanol in a fume hood at a material-to-liquid ratio of 1:8 (weight-to-volume ratio), degrease by shaking at 120 rpm at room temperature for 4 hours, replace the isopropanol, and continue shaking and degreasing at 120 rpm at room temperature for 11 hours; Discard the isopropanol, wash the isopropanol ...

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Abstract

The invention provides a biological repair membrane and a preparation method thereof. In the prior art, the existing biological repair membrane has the following disadvantages that the mechanical strength is poor, the suture point has the cerebrospinal fluid leakage risk, the tissue regeneration and repair promoting performance is weakened, the residual toxicity exists after the degradation, the biocompatibility is poor, and the adhesion between the repair material and the brain tissue is easily caused, and other complications are easily induced. According to the present invention, the animal-derived membrane tissue is subjected to cell removing and antigen removing, and a natural polymer material coating capable of being adsorbed and providing bleeding stopping effect is coated on the treated animal-derived membrane tissue so as to solve the problems in the prior art; the method specifically comprises: pretreatment, degreasing with an organic reagent, cell removing and antigen removing with an alkali liquid, coating preparation, vacuum compression and freeze-drying, packaging, and sterilization so as to complete the preparation of the biological repair membrane; and the biological repair membrane has advantages of bleeding stopping, antibacterial property, adhesion, natural three-dimensional space structure retaining, good mechanical property, good biocompatibility, good tissue growth induction, degradation non-toxicity, wide raw material source, low price, and easy mass-production.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a bioremediation membrane and a preparation method thereof. Background technique [0002] In neurosurgery, there are a large number of cases that need to be repaired due to brain (spinal) membrane damage every year, which mainly focus on dura (spinal) meningeal defects caused by tumor resection, trauma and other reasons. If not repaired in time or properly, often It causes leakage of cerebrospinal fluid, adhesion of dura mater tissue, scarring of nerve tissue and, in severe cases, epilepsy. [0003] At present, a variety of "surgical patches" have been supplied to clinical needs at home and abroad, and the most common dura (spinal) membrane replacement materials on the market are heterogeneous materials. Based on the principles of tissue engineering, the extracellular matrix made of animal tissue is The main direction of development. [0004] Chinese pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/34
Inventor 李亚婧黄文涛乔宝坤刘国强
Owner SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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