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Realizing large-volume and high-resolution temporal pulse light slice tomography method and system

A time pulse and tomographic imaging technology, which is applied in the fields of biomedicine and optical imaging to achieve fast organ-level imaging, ensure three-dimensional imaging resolution, and improve imaging depth.

Active Publication Date: 2021-01-01
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of the shortcomings of the above-mentioned background technology, the present invention proposes a time-pulse light slice tomographic imaging method and system to realize high-resolution imaging of large-volume samples or large living organisms, so as to solve the problems of light imaging resolution and imaging depth

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  • Realizing large-volume and high-resolution temporal pulse light slice tomography method and system
  • Realizing large-volume and high-resolution temporal pulse light slice tomography method and system
  • Realizing large-volume and high-resolution temporal pulse light slice tomography method and system

Examples

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Effect test

Embodiment 1

[0036] Example 1 Fluorescence labeling imaging

[0037] For fluorescent label imaging, the excitation and emission wavelengths of fluorescent labeling moieties include the visible, near-infrared, and far-infrared ranges. Taking visible light excitation as an example, the wavelength of the two laser beams is 1040 nm&1130 nm, which can be used for two-color two-photon excitation of Sulforhodamine101, Texas red, and quantum dot dyes.

[0038] Pulsed laser has the advantages of ultra-short pulse and high repetition rate. Taking femtosecond light source as an example, the laser outputs two beams of synchronous light source. The thickness of the sheet; the thickness of the time light sheet or the Z-axis resolution of imaging is determined by the time pulse width of the two beams of light pulses. Pulse light sources include attosecond, femtosecond, picosecond, and nanosecond pulse lasers. Reducing the time pulse width can achieve Higher axial resolution. The repetition frequency of t...

Embodiment 2

[0054] Example 2 Vascular Imaging

[0055] The difference between this embodiment and the first embodiment is that it adopts the certified ICG dye that can be used on the human body. Its absorption peak is at 800 nm, and it can be excited by ~1300 nm and ~2100 nm in the second near-infrared region, and the excitation light has a deeper The penetration depth of a single beam of light cannot excite fluorescence, and only a time light sheet signal is generated where the two beams of light overlap to achieve tomographic imaging of blood vessels, such as Figure 5 shown;

[0056] The sample is processed by fluorescence, phosphorescence, Raman, photothermal, and photoacoustic labeling. The two pulsed light sources alone cannot excite the labeling group to send a signal, and only when the two pulsed light sources act together can the signal be generated.

Embodiment 3

[0057] Example 3 SRS or CARS imaging of fat and protein

[0058] The difference between this embodiment and Embodiment 1 is that the sample is not fluorescently labeled, and the two light pulses λ 1 , lambda 2 Exciting endogenous molecules in the sample to emit signals when they coincide simultaneously in space and time, said signals include optical, acoustic, thermal, electrical, magnetic signals. The laser chooses pump light and Stokes light.

[0059] The specific implementation process of SRS: select the wavelength of the pump light and the Stokes light to excite specific analytical chemical bonds, modulate the Stokes light through the acousto-optic modulator, and output the reference signal of the modulator to the lock-in amplifier ; After a suitable time delay, then coincide with the pump light on the sample, and use a high-pass low-reflection dichroic mirror to reflect the pump light to the photodiode for detection on the optical path of the forward propagation of the ...

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Abstract

The invention discloses a method and system for realizing large-volume and high-resolution time-pulse optical slice tomography. The method includes: 1) the laser outputs two synchronous or phase-locked pulse light sources λ 1 , λ 2 ; 2) One of the pulse light sources λ 2 Equipped with a delay device for time delay adjustment; 3) Pulse light λ 1 , λ 2 In the direction of propagation, it is completely opposite or angled or emitted in the same direction. By adjusting the positions of the two beams of light, the overlapping of space and time pulses can be realized on the imaging sample at the same time, forming a time light sheet; 4) time light sheet The fault generates signals and provides image information; 5) By changing the relative time delay of the two beams of light or the position of the sample, the tomographic imaging of the light sheet is realized. The invention uses a modulated time-pulse light sheet to solve the problems of uneven resolution of mechanical light sheets, large defocused background and low signal-to-background ratio in depth imaging, and can be applied to large-volume organ level imaging.

Description

technical field [0001] The invention relates to the technical fields of biomedicine and optical imaging, in particular to a method and system for realizing large-volume and high-resolution time-pulse light slice three-dimensional tomographic imaging. Background technique [0002] In biomedical research and practical applications, optical imaging techniques including confocal fluorescence microscopy, multiphoton fluorescence microscopy and other imaging modalities have been widely used to observe the fine structure and functional activities of biological tissues. Although optical imaging technology has many advantages such as non-toxicity, high resolution, fast imaging speed, and high sensitivity, it is only suitable for imaging the surface layer of biological tissue from a few microns to a few millimeters in depth. For larger biological tissues or other opaque samples (1 mm to 10 cm and above), as the imaging depth increases, light scattering and absorption tend to be seriou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/00A61B5/026
CPCA61B5/0059A61B5/0073A61B5/0261A61B2576/026
Inventor 王平杨驰毕亚丽
Owner HUAZHONG UNIV OF SCI & TECH
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