Method for reducing citrus Huanglongbing pathogenic bacterium CLas (Candidatus Liberibacter asisticus) content

A technology for citrus Huanglongbing and pathogenic bacteria, which is applied in the fields of botanical equipment and methods, fungicides, chemicals for biological control, etc. , Good bactericidal effect, stable solution properties

Active Publication Date: 2020-02-04
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the beginning of the 21st century, scientists have conducted research on the prevention and treatment of Huanglongbing disease, such as hormones, nutritional interventions, small molecule target CLas virulence for osmotic stress tolerance, and plant growth regulators, but the results are still not ideal
Although the United States is under pressure to release the use of oxytetracycline (oxytetracycline, oxytetracycline) and streptomycin in Florida citrus orchards, the research and development of new antibiotics is currently lagging behind, and there is an urgent need to find new antibacterial drugs to solve the problem. current serious problems

Method used

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  • Method for reducing citrus Huanglongbing pathogenic bacterium CLas (Candidatus Liberibacter asisticus) content
  • Method for reducing citrus Huanglongbing pathogenic bacterium CLas (Candidatus Liberibacter asisticus) content
  • Method for reducing citrus Huanglongbing pathogenic bacterium CLas (Candidatus Liberibacter asisticus) content

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 carbon quantum dot nano-oxytetracycline

[0032] Preparation of carbon quantum dot nanoparticles: prepared by hydrothermal method, weighed 1.0507g of citric acid and 335μL of ethylenediamine and dissolved in 10mL of deionized water, transferred the solution to a flask (50mL), heated at 200°C overnight, after the reaction , the reactor was naturally cooled to room temperature, and the production yield was 50%. 1 mL of 10% polyacrylic acid (PAA, Sigma) was added to the carbon quantum dot solution, and heated at 80°C for 4h to obtain carbon quantum dots-polyacrylic acid. After dialysis Dialysis in the bag, vacuum drying to obtain carbon quantum dot nanoparticles;

[0033] Preparation of carbon quantum dot nano-oxytetracycline: disperse the prepared carbon quantum dot nanoparticles in water, add oxytetracycline to disperse, stir at 80°C for 1 hour, and then stir to room temperature to obtain carbon quantum dot nano-oxytetracycline, carbon Th...

Embodiment 2

[0034] The preparation of embodiment 2 iron oxide nanometer oxytetracycline

[0035] Preparation of iron oxide nanoparticles: Co-precipitation method to prepare iron oxide nanoparticles, 8.1g of FeCl 3 6H 2 O was dissolved in an appropriate amount of deionized water, and the resulting solution was transferred to a three-necked flask, stirred and heated to 70°C. 4.4 g FeCl 2 4H 2 Dissolve O in 10mL of deionized water, after filtering, take 75mL and put it into a three-neck flask, under vigorous stirring, add 18mL of 25% concentrated ammonia water, after 1min, add 4.66g of oleic acid dropwise, stir at 70°C for 1h, the reaction is complete Finally, a black jelly-like precipitate was obtained, which was separated from the reaction system by applying an external magnetic field, washed twice with ethanol to remove excess oleic acid, and washed with deionized water until neutral.

[0036] Preparation of iron oxide nano-oxytetracycline: disperse the prepared iron oxide nanoparticle...

Embodiment 3

[0037] Example 3 Characterization of Nanoparticles

[0038] Scanning electron microscope (Transmission Electron Microscopy, TEM) can observe the morphology and structure of less than 0.2μm. The TEM sample production process is as follows: cut off the carbon conductive adhesive and paste it on the sample table, dip a small amount of powder sample and stick it on the surface of the conductive adhesive and vacuumize it, and conduct morphology observation and composition analysis after carbon spraying. The fluorescence spectrum of the sample is measured by a fluorescence photometer, and the above chemical groups are detected by Fourier transform infrared spectroscopy.

[0039] The characterization results showed that the transmission electron microscopy showed that the fluorescent carbon quantum dot nanoparticles had good dispersion ( figure 1 ), the particle size distribution is relatively uniform, the size of carbon quantum dot nanoparticles is 4nm, and the size of iron oxide n...

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Abstract

The invention belongs to the technical field of plant disease prevention and treatment and particularly relates to a method for reducing citrus Huanglongbing pathogenic bacterium CLas (Candidatus Liberibacter asisticus) content. Carbon quantum dot nanoparticles are applied to preparation of carbon quantum dot nano oxytetracycline which is then injected to citrus plants with citrus Huanglongbing bymeans of trunk injection. Preparation of carbon quantum dot nano oxytetracycline includes carbon quantum dot nanoparticle preparation and carbon quantum dot nano oxytetracycline preparation. The carbon quantum dot nano oxytetracycline has the advantages of small size and is smaller than the pore diameter of a plant sieve tube in which pathogenic bacteria are parasitized, so that great bactericidal effects can be achieved; in addition, corresponding solution is stable and uniform in property and meets injection requirements. The method for reducing citrus Huanglongbing pathogenic bacterium CLas content is significant to citrus Huanglongbing prevention and treatment and has a potential of large-scale agricultural application.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control, and in particular relates to a method for reducing the content of CLas, a pathogenic bacterium suffering from citrus huanglongbing. Background technique [0002] Citrus Huanglongbing (HLB) is raging all over the world. There is still no cure for typhoon areas or citrus varieties that need protection. Once the tree is infected, it can only be cut down. Since the beginning of the 21st century, scientists have carried out studies on the prevention and treatment of Huanglongbing, such as hormones, nutritional interventions, small molecule target CLas virulence for osmotic stress tolerance, and plant growth regulators, but the results are still not satisfactory. Although the United States is under pressure to release the use of oxytetracycline (oxytetracycline, oxytetracycline) and streptomycin in Florida citrus orchards, the research and development of new antibiotics is ...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01N59/00A01N37/44A01P3/00A01P1/00
CPCA01G13/00A01N37/44A01N59/00
Inventor 姚廷山钱坤胡军华周彦周常勇
Owner SOUTHWEST UNIVERSITY
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