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New application of ferroferric oxide nano-enzyme

A technology of ferric oxide and nano-enzymes, which can be used in medical preparations of non-active ingredients, nervous system diseases, capsule delivery, etc., and can solve problems such as cognitive and memory impairment, reduction of neural differentiation, and oxidative stress

Inactive Publication Date: 2020-01-14
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when reactive oxygen species are overproduced, these molecules cause oxidative stress, reduce neural differentiation, and lead to cognitive and memory impairments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: animal model preparation

[0032] The present invention adopts male 8-week-old male ICR mice recognized by international academic circles, with a body weight of 18-22g and 2-3 months old, purchased from the Comparative Medicine Center of Yangzhou University, and kept at an appropriate temperature (23°C) and humidity (60%). 12-hour light / 12-hour dark environment, after adaptive feeding for 1 week. The mice in the normal control group normally ate and drank, and the animals were randomly divided into 5 groups (14 in each group):

[0033] 1) Control group (0.9% normal saline), 2) 100mg / kg D-galactose treatment group (D-gal-group), 3) 50mg / kg melatonin d-gal group (Mel-gal group), 4 ) 10μg / ml PEG-Fe3O4 nanozyme treatment group (pfe3O4-group); 5) 10μg / ml PEG-Fe3O4 nanozyme+100mg / kg D-gal treatment group (pFe3O4+D-gal group).

[0034] D-galactose was injected intraperitoneally at 100 mg / kg, once a day, for a total of 12 weeks to prepare the mouse model. It ha...

Embodiment 2

[0035] Example 2: Protective effect of PEG-Fe3O4 nanozyme on neurogenesis in DG region of hippocampus of mice treated with D-gal

[0036] This part adopts the animal model described in Example 1, and drinks Fe3O4 nanozyme every day for 4 weeks. After the last administration, the mouse is deeply anesthetized for 1 hour, and the brain tissue is quickly stripped, perfused with 4% paraformaldehyde, dehydrated with gradient sucrose, and the brain tissue Frozen sections were performed, and hippocampal slices were selected according to the brain atlas for immunohistochemical staining, and the morphology of neuroblasts was observed using an electron microscope.

[0037] The result is as figure 1 Shown: Doublecortin (DCX) is a microtubule-associated protein present in migrating neuroblasts, and it is one of the specific markers of early neurons. The number of DCX+ cells is related to the rate of neurogenesis, and DCX-positive cells mainly in the subgranular layer of the dentate gyrus ...

Embodiment 3

[0039] Example 3: Effect of PEG-Fe3O4 nanozyme on platelet endothelial cell adhesion molecule-1 (PECAM-1) in DG region of mouse hippocampus treated with D-gal.

[0040] This part adopts the animal model described in Example 1, and drinks Fe3O4 nanozyme every day for 4 weeks. After the last administration, the mouse is deeply anesthetized for 1 hour, and the brain tissue is quickly stripped, perfused with 4% paraformaldehyde, dehydrated with gradient sucrose, and the brain tissue Frozen sections were performed, and hippocampal brain slices were selected for immunohistochemical staining according to the brain atlas, and the expression of platelet endothelial cell adhesion molecule-1 (PECAM-1) in the vascular endothelium was observed using an electron microscope.

[0041] The result is as figure 2 Shown: the expression of PECAM-1 was easily detected in the hippocampal DG area of ​​the control group ( figure 2 A). In the D-gal group, we noticed a significant decrease in PECAM-...

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Abstract

The invention relates to a new application of ferroferric oxide nano-enzyme, which belongs to the field of western medicine pharmacy, and particularly relates to an application of polyethylene glycol-coated ferroferric oxide nano-enzyme (PEG-Fe3O4 nano-zyme) in promotion of neuroblastocyte proliferation and differentiation. After a small dose of PEG-Fe3O4 nanozyme is given to daily drinking water,the differentiation of neuroblasts in hippocampus can be increased, a blood-brain barrier is protected, autophagy is enhanced, and the oxygen free radical level is reduced. From the invention, the polyethylene glycol-coated ferroferric oxide nano-enzyme (PEG-Fe3O4 nano-enzyme) has the effect of promoting the differentiation of neuroblasts from multiple aspects, and has a certain value in the treatment of neurodegenerative diseases.

Description

technical field [0001] The invention belongs to the field of western medicine preparations, and relates to a new medicinal application of iron ferric oxide nanozyme, in particular to the application of ferric iron tetroxide nanozyme in the preparation of drugs for promoting neuroblast differentiation. Background technique [0002] Neural stem cells (neural stem cells, NSCs) have the characteristics of self-renewal and multi-directional differentiation potential, using the characteristics of NSCs, by improving the microenvironment, effectively stimulating and promoting this process, repairing and replacing damaged nerve cells in the prevention and treatment of nervous system diseases has important application prospects. Neuronal differentiation in the subgranular zone (SGZ) of the hippocampal dentate gyrus (Dg) is associated with brain cognitive reserve and brain plasticity. Reactive oxygen species are considered to be the most important oxidative molecules in cells, includi...

Claims

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Application Information

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IPC IPC(8): A61K33/26A61K9/51A61K47/10A61P25/00A61P25/28
CPCA61K9/5146A61K33/26A61P25/00A61P25/28
Inventor 颜丙春高利增高满满许卓斌王莉张洁
Owner YANGZHOU UNIV
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