Novel application of fermented activated lactobacillus carrot juice drink

A technology of active lactic acid bacteria and carrot juice, which is applied in the direction of lactobacillus, application, and the function of food ingredients, etc., can solve the problems that human health regulation effects have not been tested and studied, and achieve the effect of improving the intestinal flora structure of rats

Pending Publication Date: 2019-12-24
南达新农业股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carrot juice drinks with fermented active lactic acid bacteria with tens of billions of viable bacteria as described in this a...

Method used

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  • Novel application of fermented activated lactobacillus carrot juice drink
  • Novel application of fermented activated lactobacillus carrot juice drink
  • Novel application of fermented activated lactobacillus carrot juice drink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of Fermented Active Lactic Acid Bacteria Carrot Juice Beverage

[0030]The preparation method of the fermented lactic acid bacteria beverage is as follows: in the first step, the required amount of carrots is selected, removed the skin and non-edible parts, cleaned, sliced ​​to a thickness of 1.5 cm to 2 cm, and heated at 115 degrees Celsius to 121 degrees Celsius. Steam in high-pressure steam for 10 minutes to 15 minutes, beat the steamed carrot slices, and filter through a 40-mesh sieve to obtain carrot pulp; in the second step, 80 kg to 100 kg After adding 200 kg to 300 kg of sucrose into pure water with a temperature of 75 degrees Celsius to 80 degrees Celsius to make a sugar solution, stir and mix with 380 kg to 420 kg of carrot pulp to obtain a mixed solution; the third step is to obtain a mixture in the second step Add 0.3 kg to 0.4 kg of vitamin C to the mixed solution and mix evenly, and obtain the mixed solution by distilling the volume with pure w...

Embodiment 2

[0032] Establishment and feeding of experimental animal models

[0033] After the SD rats grew up to 8 weeks, they were divided into different groups to carry out experiments. The number of animals in each group was more than 6, with half male and half male.

[0034] The establishment method of the sleep deprivation animal model is as follows: the improved multi-platform water environment method is used to establish the sleep deprivation model, and the sleep deprivation box is self-made. Order a storage water tank of 120×70×50cm for transformation, stick 10 small plexiglass pillars with a diameter of 3cm and a height of 8cm on the bottom of the tank, and stick a circular piece with a diameter of 6.5cm on the small pillars to form a table that can make the rat stand Small platforms, the distance between each small platform is 15cm. Water at 28-30°C was injected into the water tank during modeling, and the water level was approximately 1 cm below the rat platform. There is a c...

Embodiment 3

[0035] Example 3 Extraction and sequencing of stool samples

[0036] The feces of 6 rats in each group were collected on the same day after the experiment was completed for flora sequencing analysis.

[0037] Genomic DNA extraction and sequencing method: Use CTAB or SDS method to extract the genomic DNA of the sample, then use agarose gel electrophoresis to detect the purity and concentration of the DNA, take an appropriate amount of sample in a centrifuge tube, and dilute the sample with sterile water to 1ng / μl. Using the diluted genomic DNA as a template, the 16SV4 region was amplified, and the primers used were 515F and 806R. After PCR amplification to obtain the target product, use the Ion Plus Fragment LibraryKit 48rxns library construction kit of Thermofisher to construct the library. machine sequencing.

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Abstract

The invention discloses novel application of a fermented activated lactobacillus carrot juice drink in regulating intestinal health and belongs to the technical field of nutrient functions of functional food. Intestinal health is an important health problem concerned by people at present, intestinal microbial imbalance caused by short sleep time, high pressure, metabolic disorders and other reasons is an important pathogenic factor causing multiple metabolic, internal secretion and mental diseases, therefore, intake of functional food to effectively maintain intestinal flora balance and regulate intestinal health becomes an important nutrient intervention means. The invention uses the novel fermented activated lactobacillus carrot juice drink, focuses on influences of the drink on intestinal flora structure change caused by sleep lack, obtains specific change laws of the drink which specifically changes intestinal flora structures and causes structural bacteria such as prevotellaceae,is beneficial to overall migration of intestinal flora structures away from injured structures, shows significant technical effects, can further develop new application values of the functional drinkand provides a new scheme for relieving intestinal abnormity caused by sleep lack.

Description

technical field [0001] The invention belongs to the technical field of nutritional functions of functional foods, and in particular relates to a new function and new use of a fermented active lactic acid bacteria carrot juice beverage in terms of intestinal health regulation. Background technique [0002] Intestinal health is currently an important health issue that people pay attention to. Intestinal health is largely characterized by the balance and stability of intestinal microbial structure. For a long time, people have found that more and more diseases are related to the imbalance of intestinal microorganisms, and the types of diseases and the organs of occurrence are various. In addition to irritable bowel syndrome and chronic enteritis, long-term intestinal flora imbalance can cause metabolic diseases such as diabetes, obesity, and rheumatism, and can also affect the occurrence of a series of mental diseases through brain-gut communication, such as cognition With lea...

Claims

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Application Information

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IPC IPC(8): A23L2/38A23L33/00
CPCA23L2/382A23L33/00A23V2002/00A23V2400/169A23V2200/32
Inventor 林勇马彦科徐毅林旭赵立袁征
Owner 南达新农业股份有限公司
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