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All-optical method capable of simultaneously achieving single-cell targeted drug transferring and real-time detection

A real-time detection and optical method technology, applied in the direction of material analysis, measuring devices, scientific instruments, etc. through optical means, to achieve good results, flexible operation, and fast delivery speed

Inactive Publication Date: 2019-12-06
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an all-optical method that can realize single-cell targeted drug delivery and real-time detection at the same time, which solves the problem that it is difficult to achieve fast, accurate and quantifiable micro-nano drug particles at the single-cell level in the prior art. The problem of targeted delivery and safe and efficient real-time detection, simple and convenient operation, non-toxic and non-destructive to the biological environment

Method used

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  • All-optical method capable of simultaneously achieving single-cell targeted drug transferring and real-time detection
  • All-optical method capable of simultaneously achieving single-cell targeted drug transferring and real-time detection
  • All-optical method capable of simultaneously achieving single-cell targeted drug transferring and real-time detection

Examples

Experimental program
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Effect test

Embodiment 1

[0039]1-1. This implementation example provides a manufacturing method of optical tweezers with a parabolic connection cone angle at the fiber tip and an assembling method of experimental equipment. The process is as follows.

[0040] (1) Use fiber optic pliers to cut off the coupler at one end of the fiber. Counting from the bare end, pull out the fiber coating plastic with a length of about 4dm, and then pull out the cladding with a length of about 2.5mm to obtain a bare fiber.

[0041] (2) Light the alcohol lamp, control the flame height below 1cm, then place the middle end of the bare fiber on the outer flame of the alcohol lamp for heating, after 40s the fiber is melted, draw it at a speed of 0.6mm / s for 10s, and finally use Quickly break the fiber to form a small arc parabola to connect the fiber head with a small taper angle, such as figure 1 as shown in a. If the tapered end of the optical fiber extends with a tip less than 1 μm, use a lens-cleaning paper dipped in an...

Embodiment 2

[0049] 2-1. This implementation example provides a method for stepwise targeted drug delivery and simultaneous Raman detection of a single adherent cell using fiber optic tweezers and a Raman microscope.

[0050] S1. HeLa cells were cultured in DMEM medium with 10% fetal bovine serum and 1% double antibody, and placed in an incubator with a temperature of 37°C and a carbon dioxide concentration of 5%. Twelve hours before the experiment, the HeLa cells were transferred to a cell slide with a diameter of 1.2 cm, and then placed in an incubator with the same culture environment.

[0051] S2. During the experiment, the cell slides were taken out and placed on the stage of the Raman microscope, the culture medium was sucked off, washed four times with PBS solution, and then the washed cell slides were placed on the stage of the Raman microscope Drop 1ml of PBS solution on top of the glass slide.

[0052] S3. Select silicon dioxide particles with a diameter of 3um, a pore diameter ...

Embodiment 3

[0063] 3-1. This example provides a method for simultaneous delivery and simultaneous Raman detection of multiple drug particles in a single adherent cell using optical fiber tweezers and a Raman microscope.

[0064] Steps S1-S5 are the same as S1-S5 of embodiment 2-1;

[0065] S6. Turn on the 980nm laser connected to the end of the fiber and set the power to 40mW, adjust the hexagonal adjustment frame or control the movement of the Raman microscope stage to make the tip of the fiber close to the drug particles.

[0066] S7. When the tip of the fiber is slowly approaching the drug particle by about 0.5~2 um, the fiber will capture the drug particle at the balance point at the front end of the fiber, and then continue to adjust the relative movement of the fiber or the stage so that the captured drug particle is close to the second A drug particle that needs to be captured, when the distance between the captured drug particle and the drug particle that needs to be captured is a...

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Abstract

The invention discloses an all-optical method capable of simultaneously achieving single-cell targeted drug transferring and real-time detection. Targeted drug transferring at a single-cell level andreal-time Raman detection of a micronano drug are achieved by assembling a Raman microscopy, a specially-fabricated fiber, a three-dimensional hexagonal adjustment rack and a laser. Micronano drug targeted transferring achieved by an optical control method has the advantages of rapid transfer rate and good targeting ability, quantitative transfer can be achieved, moreover, the all-optical method is wide in applicability and is flexible, simple and convenient to operate, the all-optical method can be applied to an adherent cell, and the problem that targeted drug transfer is difficult due to Brownian movement of a suspension cell also can be solved; by Raman detection, activity detection of a single cell can be achieved, a chemical reagent is not needed to dye or mark during the detection process, no damage is generated, and the all-optical method is simple, convenient and efficient; and single-cell synchronous targeted drug transfer and Raman detection which are achieved by the deviceis of important significance to the fields of cytological study, drug research and development and the like.

Description

technical field [0001] The invention belongs to the interdisciplinary technical field of optical micro-nano manipulation technology, Raman detection technology and biomedical technology, and in particular relates to an optical target for micro-nano drug particles at the single-cell level using optical fiber optical tweezers and a Raman microscope. A method for delivery and real-time detection of target cells. Background technique [0002] Single-cell targeted drug delivery and real-time detection occupy an increasingly important position in the field of cytology and biomedicine, such as drug development, tissue engineering and genetic engineering, especially cancer treatment, are inseparable from the targeting of cells Development of drug delivery and real-time detection. In order to improve the specificity, safety and effectiveness of targeted drugs, researchers have developed a variety of targeted drug systems. For example, a chemically driven micro / nanomotor consisting ...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/01G01N21/65
Inventor 雷宏香刘晓乐
Owner SUN YAT SEN UNIV
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