Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of compound bacterial strain based on hydrogen oxidizing bacteria and culture method thereof

A technology of compound strains and culture methods, applied in the field of microorganisms, can solve the problems of low strain abundance ratio and unfavorable industrial application, etc.

Active Publication Date: 2021-03-30
GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the common culture method adopts the enrichment culture method of inorganic autotrophic mode, it needs to supply CO artificially. 2 、H 2 ; If the heterotrophic mode is used to limit the medium method, the abundance ratio of the cultured strains is not high, which is not conducive to industrial application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of compound bacterial strain based on hydrogen oxidizing bacteria and culture method thereof
  • A kind of compound bacterial strain based on hydrogen oxidizing bacteria and culture method thereof
  • A kind of compound bacterial strain based on hydrogen oxidizing bacteria and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 5

[0064] This embodiment provides a method for preparing a synthetic medium of the present invention, the specific steps are as follows:

[0065] (1) The organic compound is configured into a mixed solution according to the ratio of Example 1, stirred and dispersed evenly.

[0066] (2) Minerals are configured according to the ratio of Example 1, added to the mixed solution of organic compounds, stirred and dispersed evenly, and the minerals form a suspended dispersion state in the mixed solution of organic compounds, and the turbidity is relatively high and opaque by visual inspection.

[0067] (3) adding pure water to dilute to make the total weight 100kg, continue to stir and disperse, and obtain a liquid synthetic medium after dilution.

[0068] (4) The liquid synthetic culture medium is sterilized in a cycle through an ultraviolet light device, and the sterilization time is more than 30 minutes. Put the sterilized medium into the culture container.

[0069] The medium prep...

Embodiment 6

[0073] The cultivation method of embodiment 6 composite strains

[0074] Step 1: Take peat soil with a humidity of 16% from under the vegetation in the original ecological environment of the Dinghushan National Nature Reserve in Guangdong, China, dissolve it in water, stir and dilute it, and then precipitate it, and take 1L of the supernatant as the primary strain;

[0075] Step 2: Inoculate the primary strains into the liquid medium of Example 1 for cultivation, feed compressed air, and the volume ratio relationship between liquid and air flow is 1m 3 :5-15m 3 / h, preferably, the air flow is 0.8-1m 3 / h, the temperature is controlled at 28-32°C, preferably 30-32°C. Stop the air supply after 7 days of enrichment culture, let it stand for 24 hours to settle, and separate the supernatant after the minerals precipitate to the bottom of the container to obtain the secondary strain.

[0076] Step 3: Put the secondary strains into the same reaction container; feed compressed air ...

Embodiment 7

[0087] This example adopts the cultivation method of the compound strain of Example 6, and this example uses the culture medium prepared in Example 2. The only difference between this example and Example 6 is that step 1 of this example is obtained from Mangshan, Hunan, China National Level Under the vegetation in the original ecological environment of the nature reserve, peat soil with a humidity of 30% was taken, dissolved in water, stirred and diluted, and then precipitated. Take 1L of the supernatant as the primary strain.

[0088] In this embodiment, samples were taken and tested at the culture time points of 0h, 24h, 36h, 72h, 96h, 7 days, 14 days, and 21 days, and the test results are shown in Table 6.

[0089] Table 6: Detection results at different time points during the cultivation process of Example 7

[0090]

[0091] Remarks: ①Represents Hydrogenophaga laconesensis HWB-10 T②Represents Pseudomonas wadenswilerensis CCOS 864T ③Represents Azospirillum rugosumIMMIB...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided is a composite strain based on hydrogen-oxidizing bacteria; said compound strain is a type of rhizosphere mesophilic microorganism of which all respiratory types are facultative aerobic, the suitable pH value is 4.5–8, the suitable water activity is 0.8–0.9, and the nutritional type is chemotrophic organic / inorganic facultative heterotrophic. Also provided is a method for culturing the composite strain; as common survival conditions for the compound strain are provided, fungi decompose organic carbon-containing compounds into organic acids and CO2 to provide hydrogen-oxidizing bacteria as a carbon source for a heterotrophic mode; nitrogen-fixing bacteria convert N2 in the air into NH3 and simultaneously release H2; the hydrogen-oxidizing bacteria use organic acids, CO2, NH3, H2, and O2 in the air as their metabolic elements to achieve energy transfer and balance, and, by means of enrichment and cultivation, a composite strain based on hydrogen-oxidizing bacteria is obtained. By means of co-cultivating hydrogen-oxidizing bacteria, nitrogen-fixing bacteria, and fungi, a compound strain having plant growth-promoting function is obtained, and can be used as a high-efficiency organic bacterial fertilizer.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite strain based on hydrogen oxidizing bacteria and a cultivation method thereof. Background technique [0002] Microbial preparations have important and extensive applications in the field of agriculture and forestry. They can promote plant biological nitrogen fixation, improve soil fertility, increase the absorption and utilization of plant mineral elements, enhance plant disease resistance, drought resistance, increase yield, and improve quality. It plays an irreplaceable role in green agriculture and ecological agriculture. Root growth-promoting microorganisms (PGPR) are the combined growth-promoting mechanism of various microorganisms to promote plant growth, and are also the focus of research in the field of agricultural microorganisms. The research and application of microbial preparations are mostly concentrated in the fields of rhizobia, nitro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/20A01N63/27A01N63/30A01P21/00C05F11/08C05G3/00C05G3/80C12R1/01C12R1/38C12R1/645
CPCA01N63/00C05F11/08C12N1/14C12N1/20
Inventor 韩允奉袁文豪
Owner GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com