Special beta-dextranase-containing complex enzyme for piglet and preparation method thereof
A dextranase and compound enzyme technology, applied in biochemical equipment and methods, enzymes, applications, etc., can solve the problems of reducing the content of antigenic crude protein in the diet, lack of innate immunity in newborn piglets, and imperfect development of the digestive system , to achieve good stability and preservation activity, improve digestion utilization, and natural antibacterial immunity
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Embodiment 1
[0041] The preparation method of embodiment 1 Chinese herbal medicine powder
[0042] A preparation method of Chinese herbal medicine powder is as follows:
[0043] Weigh 25 parts of Radix Astragali; 15 parts of Codonopsis; 12 parts of Bupleurum; Keep at ℃ for 3h, then cool down to 50℃, add mixed enzyme preparation for enzymolysis, use lactic acid to adjust the pH value to 6, enzymolysis for 3h, finally add a mixture of ethanol and propanol twice the weight of the mixed material, 11W ultrasonic extraction for 1h, filter ; The filtrate was concentrated in vacuum and then freeze-dried to obtain Chinese herbal medicine powder.
[0044] The added amount of the mixed enzyme is 8% of the total weight of the mixed material.
[0045] The weight part array of described mixed enzyme is: 12 parts of β-glucosidase, 18 parts of xylanase, 18 parts of pentosanase, 25 parts of pullulanase, 18 parts of β-amylase, 12 parts of pectinase 12 parts of acid protease, 8 parts of papain, 8 parts of...
Embodiment 2
[0047] Embodiment 2 A kind of preparation method of β-glucanase
[0048] A preparation method for β-glucanase, comprising the steps of:
[0049] (1) Expanded cultivation of liquid seeds
[0050] ①First-level seed culture: put the activated slant strains into 500 ml shake flasks with 2 rings, the medium capacity is 100 ml, the rotary shaker is 180 rpm, the culture temperature is 40°C, and the culture time is 10 hours;
[0051] ②Cultivation of secondary seeds: Inoculate 10% of primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as primary seeds;
[0052] ③Third-level seed cultivation: Inoculate the second-level seeds with 10% inoculum amount into a 5000 ml third-level seed shaker flask, the medium capacity is 1000 ml, the rotary shaker is 100 rpm, the culture temperature is 40°C, and the culture time is 12 hours ;
[0053] ④First-level seed tank cultivation: Put the third-level seeds into the first-level seed tank with a total vo...
Embodiment 3
[0062] Example 3 A preparation method of a special compound enzyme for suckling pigs containing β-glucanase
[0063] A special compound enzyme for suckling piglets containing β-glucanase, which consists of the following enzyme preparations in parts by weight:
[0064] 10 parts of Chinese herbal medicine powder, 1000 parts of xylanase, 100 parts of cellulase, 220 parts of β-glucanase, 20 parts of mannanase, 1000 parts of protease, 75 parts of medium temperature α-amylase.
[0065] Get xylanase, cellulase, mannanase, medium-temperature α-amylase, protease from Hunan Hongyingxiang Biological Engineering Co., Ltd. according to the above parts by weight, and the Chinese herbal medicine powder obtained in Example 1, implement The β-glucanase obtained in Example 2 was stirred evenly in a mixer at 25°C for 5 minutes. Stir evenly and store in a box at room temperature or in a cold room.
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