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Compound strain based on hydrogen hydroxide bacteria and culture method thereof

A technology of compound strains and culture methods, applied in the field of microorganisms, can solve the problems of low abundance ratio of strains and unfavorable industrial application

Active Publication Date: 2019-12-06
GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the common culture method adopts the enrichment culture method of inorganic autotrophic mode, it needs to supply CO artificially. 2 、H 2 ; If the heterotrophic mode is used to limit the medium method, the abundance ratio of the cultured strains is not high, which is not conducive to industrial application

Method used

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  • Compound strain based on hydrogen hydroxide bacteria and culture method thereof
  • Compound strain based on hydrogen hydroxide bacteria and culture method thereof
  • Compound strain based on hydrogen hydroxide bacteria and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 5

[0064] This embodiment provides a method for preparing the synthetic medium of the present invention, and the specific steps are as follows:

[0065] (1) The organic compound is prepared according to the ratio of Example 1 and the mixed solution is stirred and dispersed evenly.

[0066] (2) The minerals are configured according to the ratio of Example 1, added to the mixed liquid of organic compounds, stirred and dispersed uniformly, and the minerals form a suspended dispersion state in the mixed liquid of organic compounds, with high visual turbidity and no light transmission.

[0067] (3) Add pure water to dilute to make the total weight 100kg, continue to stir and disperse, and obtain a liquid synthetic medium after dilution.

[0068] (4) The liquid synthetic medium is sterilized in cycles through an ultraviolet light device, and the sterilization time is> 30 min. Put the sterilized medium into the culture container.

[0069] The culture medium prepared in Examples 1 to 4 was tested...

Embodiment 6

[0073] Example 6 Cultivation method of composite strain

[0074] Step 1: From under the vegetation of the original ecological environment in Dinghushan National Nature Reserve, Guangdong, China, take the high humidity turf soil with a humidity of 16%, stir and dilute with water to precipitate, and take 1L of the supernatant as the primary strain;

[0075] Step 2: Inoculate the primary strains into the liquid medium of Example 1 for cultivation, and pass in compressed air. The volume ratio of liquid to air flow is 1m 3 :5-15m 3 / h, preferably, the air flow is 0.8-1m 3 / h, the temperature is controlled to 28-32°C, preferably 30-32°C. After 7 days of enrichment culture, the gas supply is stopped, and the precipitation is allowed to stand for 24 hours. After the minerals settle to the bottom of the container, the supernatant is separated to obtain the secondary strain.

[0076] Step 3: Take the secondary strains and put them into the same reaction vessel; pass in compressed air with an a...

Embodiment 7

[0087] This example uses the method for culturing the composite strain of Example 6. This example uses the culture medium prepared in Example 2. The only difference between this example and Example 6 is that the first step of this example is from the national level of Mangshan, Hunan, China. Under the vegetation of the original ecological environment of the nature reserve, take 30% high-humidity turf soil, dissolve the water, stir and dilute it to precipitate, and take 1L of supernatant as the primary strain.

[0088] In this example, samples were taken at the culture time points 0h, 24h, 36h, 72h, 96h, 7 days, 14 days, and 21 days for testing. The test results are shown in Table 6.

[0089] Table 6: Test results at different time points in the cultivation process of Example 7

[0090]

[0091] Remarks: ①Represents Hydrogenophaga laconesensis HWB-10 T ②Represents Pseudomonas wadenswilerensis CCOS 864T ③Represents Azospirillum rugosumIMMIB AFH-6T ④Represents Varicosporellopsis aquatil...

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Abstract

The invention provides a compound strain based on hydrogen hydroxide bacteria. The compound strain is a root circle mesophilic microorganism with facultative aerobic respiratory type, suitable pH value of 4.5-8, suitable water activity of 0.8-0.9, and organic / inorganic facultative chemoheterotrophic nutrient type. The invention also provides a culture method of the compound strain, and the methodcomprises the following steps of: under the common survival condition of the compound strain, fungi decompose organic carbon-containing compounds into organic acid and CO2 which are provided to the hydroxide bacteria as a carbon source in a heterotrophic mode; azotobacter converts N2 in the air into NH3 and simultaneously releases H2; and the hydrogen hydroxide bacteria utilize organic acid, CO2,NH3, H2 and O2 in air as metabolic elements to realize energy transfer and balance, and the compound strain based on the hydrogen hydroxide bacteria is obtained through enrichment culture. Accordingto the invention, the compound strain with a plant growth promoting function is obtained by carrying out combined culture on the hydrogen hydroxide bacteria, the azotobacter and the fungi, and can beused as a high-efficiency organic bacterial fertilizer.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a composite strain based on hydrogen-oxidizing bacteria and a cultivation method thereof. Background technique [0002] Microbial preparations have important and extensive applications in the field of agriculture and forestry. They can promote plant biological nitrogen fixation, improve soil fertility, increase the absorption and utilization of plant mineral elements, enhance plant disease resistance, drought resistance, increase yield, and improve quality. Green agriculture and ecological agriculture play an irreplaceable role. Root Growth Promoting Microorganisms (PGPR) is a joint growth promotion mechanism of multiple microorganisms to promote plant growth, and is also the focus of research in the field of agricultural microbiology. The research and application of microbial preparations are mostly concentrated in the fields of rhizobia, nitrogen-fixing bacteria, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/00A01N63/04A01P21/00C05F11/08C12R1/01C12R1/38C12R1/645
CPCA01N63/00C05F11/08C12N1/14C12N1/20
Inventor 韩允奉袁文豪
Owner GUANGDONG RICHHOLD BIOLOGICAL AGRI CO LTD
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