Method for simultaneously detecting 22 sugars, sugar alcohol and alcohol in fruit juice
A technology for fruit juice and sugar alcohol, applied in the field of analytical chemistry, can solve the problems of inability to meet the detection requirements and the number of components is small, and achieve the effects of reducing the detection cost, improving the accuracy and improving the detection efficiency
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Embodiment 1
[0046] Embodiment 1: a method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice
[0047] 1. Instruments and Reagents
[0048]ICS5000+ ion chromatography system is equipped with electrochemical detector and autosampler (Thermo Scientific, USA); Milli-Q ultrapure water instrument (Millipore, USA); electronic balance (Mettler ML802 / 02); experimental water: Ultrapure water (resistivity 18.2MΩ.cm); sugar, sugar alcohol, alcohol standard or standard reagent (purity >98%); sodium hydroxide solution (50% w / w, Fisher Chemical); hydrochloric acid (excellent grade pure); IC-RP10 column (Agela Technologies).
[0049] 2. Ion Chromatography Conditions
[0050] CarboPac MA1 (4×250 mm) analytical column, CarboPac MA1 (4×50 mm) guard column; working electrode: Gold (Au); reference electrode: AgCl electrode; mobile phase: NaOH eluent (nitrogen protection); flow rate : 0.40 mL / min; column temperature: 30 ℃; injection volume: 10 μL. Initial rinse con...
Embodiment 2
[0058] Example 2: Influence of flow rate on detection
[0059] According to the conditions of the experimental analysis in Example 1, under the condition that other conditions remain unchanged, the flow rate of the eluent is successively tested to be 0.30 mL / min, 0.40 mL / min, 0.50 mL / min, and 0.60 mL / min. The separation of the components of the standard solution, the relationship between the flow rate of the eluent and the system pressure is shown in Table 2.
[0060] Table 2 Relationship between eluent flow rate and system pressure
[0061]
[0062] From Table 2, it can be concluded that when the eluent flow rate is 0.30 mL / min, the system pressure is 885 psi, because the flow rate is lower, the pressure is lower, the peak width increases, the peak sensitivity decreases, and the overall resolution is not high. When the flow rate was 0.50 mL / min, the system pressure reached 1430 psi. Although the component sensitivity was improved, the resolution of individual components w...
Embodiment 3
[0063] Example 3: Influence of multi-stage gradient elution conditions on detection
[0064] According to the conditions of the experimental analysis in Example 1, under the condition of other conditions unchanged, test the initial eluent concentration successively and mix the standard solution components under the conditions of 420, 450, 480, 500, 520, 550 mmol / L respectively Separation situation, since the initial concentration has little effect on the resolution of the three components of components 20, 21, and 22, here we focus on the impact of the initial concentration on the first 19 components, and the component separation of the mixed standard solution is as follows figure 2 shown.
[0065] From figure 2 It can be seen from the figure that when the initial eluting concentrations were 420 and 450 mmol / L, components 7 and 8, and components 10 and 11 were not separated; when the initial eluting concentrations were 480 mmol / L, components 10 and 11 The resolution was no...
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