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Pre-mineralization natural polysaccharide based hydrogel and preparation method and application thereof

A natural polysaccharide and hydrogel technology, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of unreachable and expensive tooth sensitive restoration materials, and achieve easy operation, promote wound healing, and controllable gel performance Effect

Inactive Publication Date: 2019-11-12
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to reduce the damage to normal tissues, solve the problems of expensive tooth-sensitive restoration materials and the inability to achieve the purpose of tissue engineering remineralization, a new type of tissue with sufficient source of raw materials, easy operation, low or no immunogenicity should be developed. Engineering materials, combined with calcium phosphate salts and bioactive molecules, can repair tooth defects and seal tooth tubules to relieve tooth sensitivity symptoms, and at the same time can mineralize in situ, simulating the dentin regeneration process for dental caries treatment It is an important direction for the development of today's stomatology medical materials

Method used

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  • Pre-mineralization natural polysaccharide based hydrogel and preparation method and application thereof
  • Pre-mineralization natural polysaccharide based hydrogel and preparation method and application thereof
  • Pre-mineralization natural polysaccharide based hydrogel and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0067](1) Dissolve chitosan (CS) in 0.05mol / L acetic acid solution, the concentration of chitosan in acetic acid solution is 10mg / mL, put the dissolved CS solution in a three-necked flask, repeat according to CS The molar ratio of unit to maleic anhydride is 1:1 and maleic anhydride is added (dissolve maleic anhydride in 15ml of deionized water, add in three times, the amount of each addition is the same, and the interval between each addition is 5min), nitrogen, React at room temperature in the dark for 24 hours, then install it with a dialysis bag (φ5000-8000KDa) and place it in the dialysate, and dialyze in the dark for 4 days, in which the dialysate on the first, third, and fourth days is deionized with a pH of 6.0 Water, the dialysate on the second day is a NaCl solution with a pH of 6.0 and a mass concentration of 1%. Replace the dialysate every 12 hours to remove impurities or unreacted small molecules. Freeze-dry at 80° C. for 24 hours to obtain maleylated chitosan, wh...

Embodiment 2

[0079] The premineralized hydrogels MS-CaP-30, MS-CaP-50 and MS-CaP-70 obtained in Example 1 were subjected to in vitro dialysis mineralization, specifically: the above premineralized hydrogels were premineralized at -80°C. Cold for 12 hours, freeze-dried at -80°C for 24 hours to obtain a freeze-dried gel h; dialyze the freeze-dried gel with an alkaline phosphatase solution with a mass concentration of 4% (the pH of the solution was adjusted to = 8.0 by 1mol / L NaOH solution) Glue, dialyzed for 24 hours, the dialyzing temperature is 60°C, and the dialysis bag is φ5000~8000KDa. Afterwards, the hydrogel was rinsed with deionized water until it was neutral, then freeze-dried, and freeze-dried again at -80°C for 24 hours to obtain a mineralized natural polysaccharide-based hydrogel scaffold material containing hydroxyapatite, the main component of human hard tissue. According to the sequence of 30%, 50% and 70% of the total mass content of the added inorganic matter, the correspond...

Embodiment 3

[0083] The premineralized natural polysaccharide-based hydrogel MS-CaP-50 in Example 1 was pre-cooled at -80°C for 12h, and then freeze-dried at -80°C for 24h to obtain a freeze-dried gel. Alkaline phosphatase solution (the pH of the solution was adjusted to = 8.0 by 1mol / L NaOH solution) was dialyzed to freeze-dried gel, the dialysis temperature was 60°C, the dialysis bag was φ5000-8000KDa, and the dialysis time was 12h, 24h, 48h, 72h and 96h. Afterwards, the hydrogel was rinsed with deionized water until it was neutral, then freeze-dried, and then freeze-dried again at -80°C for 24 hours to finally obtain a mineralized natural polysaccharide-based hydrogel scaffold material containing hydroxyapatite, the main component of human hard tissue. According to the sequence of dialysis time of 12h, 24h, 48h, 72h and 96h, they are labeled as MMS-12, MMS-24, MMS-48, MMS-72 and MMS-96.

[0084] MMS-0 in Comparative Example 2 of the same volume, MMS-30, MMS-50, MMS-70 in Example 2, and...

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Abstract

The invention discloses pre-mineralization natural polysaccharide based hydrogel and a preparation method and application thereof. The preparation method comprises the steps of enabling calcium phosphorus salt to dissolve in an acylation natural polysaccharide material aqueous solution, then adding a sulfhydrylation sodium alginate polysaccharide material aqueous solution, performing uniform mixing, adding a beta-sodium glycerophosphate solution, and adjusting pH to 7.0-8.0 so as to obtain the pre-mineralization natural polysaccharide based hydrogel. After the pre-mineralization natural polysaccharide based hydrogel is subjected to freeze drying, dialysis in an alkaline phosphatase solution of which the mass concentration is 1-10% is performed for 12-48h, and freeze drying is performed sothat a hydroxyapatite-contained mineralization natural polysaccharide based hydrogel support material is obtained. The prepared pre-mineralization gel can be subjected to in situ mineralization, and calcium phosphorus salt crystals similar to natural hydroxyapatite in teeth are obtained; besides, the prepared pre-mineralization natural polysaccharide based hydrogel can effectively and uniformly penetrate into the inner parts of canaliculi dentales, so that trouble caused by odonthemodia due to dental caries to patients can be overcome, and damaged dentine and dental enamel structures are repaired.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a premineralized natural polysaccharide-based hydrogel and a preparation method and application thereof. Background technique [0002] Dental caries is an extremely common chronic, progressive, and destructive disease that occurs in the mineralized tissues of teeth, with bacteria as the pathogen and many other etiologies. Clinically, the integrity of the enamel on the surface of the tooth is destroyed, and gradually eroded dentin and even the pulp form cavities. When encountering cold and hot stimuli, there will be sensitivity symptoms, and more serious lesions of the pulp and periapical tissue will be caused. Caries usually occurs on the exposed tooth surface. Existing bacteria adhere to the tooth surface by means of a saliva film that has formed on the tooth surface and form a plaque biofilm. The bacteria inside use the sugar that enters the plaque to produce it th...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/12A61L27/02A61L27/50A61L27/52A61L27/54A61L27/56C08J3/075C08L5/04C08L5/08
CPCA61L27/025A61L27/12A61L27/20A61L27/50A61L27/52A61L27/54A61L27/56A61L2300/412A61L2430/02A61L2430/12C08J3/075C08J2305/04C08J2305/08C08J2405/04C08J2405/08C08L5/04C08L5/08
Inventor 李立华张舒昀赵要武
Owner JINAN UNIVERSITY
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