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Antitumor platinum drug mineralization protein nanoparticles and preparation method and application thereof

A platinum drug and nanoparticle technology, which is applied in the field of biomedicine to achieve good tumor cytotoxicity and reverse tumor drug resistance.

Active Publication Date: 2019-10-25
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in this field, there is an urgent need to develop a new type of platinum-based drug protein nanoparticle with clinical application prospects and tumor targeting, so as to realize the tumor-targeted delivery of platinum-based drugs and overcome the resistance of platinum-based drugs. to improve the anti-tumor effect of platinum-based drugs

Method used

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  • Antitumor platinum drug mineralization protein nanoparticles and preparation method and application thereof
  • Antitumor platinum drug mineralization protein nanoparticles and preparation method and application thereof
  • Antitumor platinum drug mineralization protein nanoparticles and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Preparation of platinum-based drug mineralized protein nanoparticles, the specific steps are as follows:

[0056] Synthesis of platinum-based prodrug compounds: cisplatin and silver nitrate were mixed in water at a molar ratio of 1:2, reacted at 60 °C in the dark for 3 hours, then cooled to room temperature, continued to react for 10 hours, and then reacted at 12000 rpm The sample was centrifuged at a rotating speed for 12 minutes to remove the silver chloride precipitate, and the obtained supernatant was an aqueous solution of platinum-based prodrug compound.

[0057] Take 100 mM platinum-based prodrug compound aqueous solution and 13.3 mg / mL human serum albumin (HSA) aqueous solution and mix thoroughly according to the volume ratio of 1:5, the molar ratio of platinum-based prodrug compound to human serum albumin is 100:1 , use 0.2 M NaOH solution to adjust the pH of the mixed solution to 6.4, then add potassium iodide (KI) aqueous solution to introduce ligand ion iodi...

Embodiment 2

[0061] The steps of this example are the same as in Example 1, the difference is that the molar ratio of the platinum prodrug compound to the ligand ion is 1:4, and the prepared nanoparticles are photographed with an electron microscope, and the results are as follows figure 2 As shown, the average particle size of the prepared nanoparticles is 4.1±0.3nm.

Embodiment 3

[0063] The steps of this example are the same as in Example 1, the difference is that the molar ratio of the platinum prodrug compound to the ligand ion is 1:2, and the prepared nanoparticles are photographed with an electron microscope, and the results are as follows image 3 As shown, the average particle size of the prepared nanoparticles is 2.3±0.2nm.

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Abstract

The invention discloses antitumor platinum drug mineralization protein nanoparticles and a preparation method and application thereof. The antitumor platinum drug mineralization protein nanoparticlesand the preparation method thereof have the advantages that the platinum drug mineralization protein nanoparticles are successfully prepared through the two-step preparation method for the first time,the prepared nanoparticles can be uniformly dispersed in an aqueous solution, the nanoparticles has good cytotoxicity, and the IC50 of the nanoparticles to human non-small-cell lung cancer cells A549is 6.9 microgram / ml; the platinum drug mineralization protein nanoparticles comprising a platinum drug and protein are simple in preparation process, uniform in size, controllable in particle size, good in biocompatibility, good in water solubility, long in blood circulation time, high in tumor targeting performance and capable of laying a foundation for efficient tumor treatment or drug-resistant tumor treatment.

Description

technical field [0001] The invention belongs to biomedicine technology, and in particular relates to a platinum drug mineralization protein nanoparticle and its preparation method and application. Background technique [0002] Platinum drugs are commonly used clinical anti-tumor chemotherapy drugs, which have broad-spectrum anti-tumor effects. Platinum drugs also have many deficiencies, such as poor curative effect, high toxicity and side effects, poor targeting, and prone to tumor drug resistance. Nano-drug carriers (such as polymer micelles, vesicles, etc.) can improve this. However, platinum-based antineoplastic drugs commonly used in clinical practice (such as cisplatin and its derivatives) are difficult to pass through due to their poor water and oil solubility. Traditional nanocarrier entrapment usually uses chemical coupling or prodrug modification to achieve its loading in the carrier, but these nano-drug carriers often have disadvantages such as difficult to effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K9/52A61K31/555A61K33/243A61K47/42A61P35/00
CPCA61K9/0019A61K9/5169A61K31/555A61P35/00A61K33/243
Inventor 陈华兵邓益斌杨红柯亨特刘玲李厅杨涛王璐
Owner SUZHOU UNIV
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