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A kind of method for cultivating virus-free seedlings of olive oleracea female plants

A cultivation method, the technology of Lavender oleracea, which is applied in the field of plant cultivation, can solve the problems of low survival rate and shortage of Lamella oleracea resources, and achieve the effects of fast growth, alleviating the contradiction between supply and demand of medicinal materials, and restoring the original traits of plants

Active Publication Date: 2022-03-11
湖北金水源农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of the shortage of golden fruit olive resources and the low survival rate caused by the uneradicated virus existing in the existing artificial seedling breeding technology, and to provide a method for cultivating virus-free seedlings of golden fruit olive female plants

Method used

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  • A kind of method for cultivating virus-free seedlings of olive oleracea female plants
  • A kind of method for cultivating virus-free seedlings of olive oleracea female plants
  • A kind of method for cultivating virus-free seedlings of olive oleracea female plants

Examples

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Embodiment 1

[0041] A method for cultivating virus-free seedlings of olive oleracea female plants, comprising the following steps:

[0042] 1) Selection of female plants of P. japonica: female plants of P. chinensis in the wild state, with normal flowering and fruiting, strong plants, and no pests and diseases 2-3 years before collection, are selected as the material collection objects; the collection time is selected at From March to June every year, before the plant blooms, select the vigorously growing stem tip of the main stem or side stem as the explant;

[0043]2) Disinfection of the surface of the stem tip of the female Lavender plant: cut the collected stem tip to 1-1.5 cm, add detergent to tap water to clean the dirt attached to the surface, rinse with tap water for 10 minutes, and soak in 0.3% bromogeramine solution The surface is sterilized for 20 minutes, rinsed with pure water for 2 hours, and transferred to a pre-prepared ultra-clean workbench for later use. Soak the explant...

Embodiment 2

[0053] Compared with Example 1, the soaking time of the explants in the 0.1% mercuric chloride solution in step 2) was 20 minutes.

Embodiment 3

[0055] Compared with Example 1, the soaking time of the explants in the 0.1% mercuric chloride solution in step 2) was 18 minutes.

[0056] Below by using conventional 75% alcohol to soak the explants for 30 seconds to disinfect, rinse and then carry out aseptic inoculation after stem tip stripping as a control group, and adopt three embodiments of the present invention to detoxify the female plant of Lavenderia aurea The domestication situation is compared, and each group of detoxification treatment is 100 bottles, and the detoxification seedlings of the control group and the three embodiments are respectively transplanted to the greenhouse for domestication, and a comparative experiment is done under the same conditions, and the results obtained are as follows:

[0057] Group Item shoot tip survival rate Reproductive coefficient detoxification rate domestication survival rate Control group 1 0 0 0 Embodiment one 78 83.5 98.3 Embodiment two...

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Abstract

The invention discloses a method for cultivating virus-free seedlings of the female plant of Lavenderia, which comprises the following steps: selecting the female plant of Larvae, disinfecting the surface of the stem tip of the female plant of Larvae, and the stem tip of the female plant of Larvae Stripping, virus detection and establishment of virus-free strains, rapid propagation of virus-free seedlings of female plants of Laura quinquefolius, domestication and cultivation of virus-free seedlings of female plants of Larva quinquefolius. The present invention selects the female plant of Laura quinquefolius as the collection object, and the domesticated and obtained are all the female plants of Lagna quinquefolium, which will bloom and bear fruit after planting; the meristem at the growth point of the shoot tip of the plant is used, and the cells in this part contain less virus Or virus-free, and passed the virus test, the produced seedlings are virus-free, can restore the original characteristics of the plant, significantly improve the tuber quality and yield of Lavender, and reduce the planting risk caused by diseases and insect pests; the present invention Utilizing the totipotency of plant cells, a complete aseptic propagation system is established through the multiplication and cultivation method of the invention, which greatly improves the yield and quality of seedlings.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, and in particular to a method for cultivating virus-free seedlings of Larva oleracea female plants. Background technique [0002] Jinguolan is an evergreen twining vine belonging to the genus Tinobilis. It is mainly produced in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other places. Tonsillitis, acute gastroenteritis, carbuncle, diarrhea, dysentery, abdominal pain, etc. It can be made into ointment for external use, rubbed with juice or ground into powder to treat diseases such as phlebitis and venomous snake bites. The root tuber contains palmatine, columbin, isocolumbin, jatrrhizine, and tinoside. Roots contain palmitine, jatrorrhizine, coumbamine, stepharanine, dehydrodiscretamine, menisperine, magnolialine (magnoflorine), 2-deoxychitosterone (2-deoxycrustecdysone), 2-deoxy-3-epicrustecdysone (2-deoxy-3-epicrustecdysone), 2-deoxychitosterone-3-0- β-glucopyranoside (2-deoxycrust...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韩明清吴昊吴顺伟尚长青
Owner 湖北金水源农业开发有限公司
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