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Bacillus amyloliquefaciens JT68 and application thereof in prevention and control of tea anthracnose

A technology for desolvating amyloliquefaciens and JT68, which is applied to Bacillus amyloliquefaciens JT68 and its application in preventing and controlling tea anthracnose, can solve problems such as differences in effects, and achieve the effects of reducing spore germination, reducing pesticide residues, and inhibiting mycelial growth.

Active Publication Date: 2019-10-11
海峡两岸农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the control effects of different Bacillus amyloliquefaciens strains on pathogenic fungi are specific and different, both in terms of antibacterial spectrum and antibacterial effect, and even the effects of different strains of individuals are also quite different

Method used

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  • Bacillus amyloliquefaciens JT68 and application thereof in prevention and control of tea anthracnose
  • Bacillus amyloliquefaciens JT68 and application thereof in prevention and control of tea anthracnose
  • Bacillus amyloliquefaciens JT68 and application thereof in prevention and control of tea anthracnose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and Screening of Bacillus amyloliquefaciens JT68

[0034] 1. Sample collection:

[0035] The soil samples were collected from the paddy field soil of the school farm of South China Agricultural University (the location is Yuejin North Farm of South China Agricultural University, Tianhe District, Guangzhou City, Guangdong Province). Fill the paddy field soil with 50mL sterilized centrifuge tube and take it back to the laboratory for isolation.

[0036] 2. Separation of biocontrol bacteria:

[0037] Mix each soil sample evenly, divide into packages and arrange numbers, take 1 / 3 of the soil samples in 10mL centrifuge tubes, add sterilized water to 10mL, vibrate vigorously to make a suspension, and record the concentration of the obtained suspension as 1 ×10 -1 . Then in 80 ° C water bath for 15 min. Draw 1mL 1×10 -1 The soil sample suspension into a new centrifuge tube filled with 9mL sterile water, diluted to 1 × 10 -2 , 1×10 -3 series concentr...

Embodiment 2

[0045] The identification of embodiment 2 bacillus amyloliquefaciens JT68

[0046] 1. Morphological identification

[0047] The bacterial strain JT68 obtained by screening in Example 1 is cultivated on LB medium, and the morphological characteristics are observed: a single colony is a regular circle, white, milky, and the colony is relatively large with a smooth surface. Electron microscopy showed that the bacteria were rod-shaped, twins, with a diameter range of 2.0*5.8um (such as figure 1 ).

[0048] Physiological and biochemical identification results found that the strain JT68 is a Gram-positive bacterium, which can utilize sucrose, fructose, mannitol and maltose. The physiological and biochemical tests such as contact enzyme reaction, motility measurement, starch hydrolysis, V-P test, nitrate reduction, and gelatin liquefaction showed positive results. Positive, unable to utilize glucose and lactose, aerobic, oxidase reaction, methyl red test, hydrogen sulfide test, etc...

Embodiment 3

[0055] The preparation of embodiment 3 bacillus amyloliquefaciens JT68 fermented liquid

[0056] This example provides a scheme selection for preparing the fermentation broth of Bacillus amyloliquefaciens JT68.

[0057] First, the preserved strains were activated from a -80°C freezer. The activated colonies were picked with an inoculation loop and cultured at 30°C in LB liquid medium (yeast extract 5g, tryptone 10g, NaCl 5g, water 1000mL, pH 7.2, sterilized at 121°C for 15min), which was the seed liquid.

[0058] Then, take 1 mL of seed solution and add it to 100 mL of LB liquid medium, and culture at 30° C. at 180 r / m for 24 hours to obtain a fermentation liquid.

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Abstract

The invention discloses bacillus amyloliquefaciens JT68 and application thereof in prevention and control of tea anthracnose. The screened bacillus amyloliquefaciens JT68 can significantly inhibit themycelial growth and spore germination of Gloeosporium theae-sinensis Miyake and reduce disease indexes, has a very good inhibitory effect on the Gloeosporium theae-sinensis Miyake, and is expected tobe used for preventing and controlling tea anthracnose and reducing the occurrence of tea anthracnose. The bacillus amyloliquefaciens is derived from Guangdong province and has a higher application value in prevention and control of local tea anthracnose, especially for local tea gardens suffering tea anthracnos at a high incidence rate. At the same time, the bacillus amyloliquefaciens JT68 alsohas a good inhibitory effect on rice blast fungi, brassica parachinensis anthracnose fungi, banana wilt fungi, pepper anthracnose fungi, cotton fusarium wilt fungi and the like, and has a prospect indevelopment of a fungicide for inhibiting plant pathogenic fungi and prevention and control of tea anthracnose. The bacillus amyloliquefaciens can not only reduce the use of chemical pesticides but also reduce the pesticide residues of tea, and a new way is provided for gradually replacing chemical prevention and control with biological prevention and control.

Description

technical field [0001] The invention belongs to the technical field of plant disease control. More specifically, it relates to a Bacillus amyloliquefaciens JT68 and its application in preventing and treating tea anthracnose. Background technique [0002] According to statistics, the global food loss caused by pests and diseases can reach 10% of the total amount every year (FAO data). Chemical pesticides are the main measures to control plant diseases and insect pests. However, the excessive use of chemical agents has led to increased resistance to diseases and insect pests, serious pesticide residues in agricultural products, damage to the soil environment, and a reduction in the number of beneficial natural enemies. As far as agricultural commodity trade is concerned, pesticide residues in agricultural products directly restrict product sales and foreign trade. This is very common in the export trade of tea. Due to the use of a large amount of chemical pesticides in the...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00C12R2001/07C12N1/205A01N63/10
Inventor 舒灿伟张懿梁均钿周而勋杨媚刘少群习平根廖美德常长青
Owner 海峡两岸农业科技股份有限公司
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