Device and method for fast synthesizing ectotrophic mycorrhiza
An ectomycorrhizal and rapid technology, applied in the fields of botany equipment and methods, horticulture, plant cultivation, etc., can solve the problems of cumbersome methods and long time, and achieve the effect of increasing the infection rate, reducing the dosage and reducing the cost.
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Embodiment 1
[0028] A device for rapidly synthesizing ectomycorrhizae, comprising a seedling-growing room, the outside of the seedling-growing room 1 is a square petri dish, and two small openings of 2 cm × 1 cm are opened on one side thereof; Stretching the bacterial agent carrying film 2, on which the ectomycorrhizal fungal inoculum 3 is attached; the mycorrhizal fungal inoculum 3 is in contact with the root system of the seedling stock 4.
[0029] Use device among the present invention to cultivate ectomycorrhizal black pine, comprises the steps:
[0030] 1) Cultivate black pine seedlings: buy new seeds collected in the autumn of the year, soak and disinfect with 10% (volume ratio) hydrogen peroxide for 30 minutes. Soak in deionized water for 30 minutes, and place in a dark incubator at 35°C for 24 hours to accelerate germination. The seeds were sown in a square sieve (40 cm×25 cm×10 cm) filled with sterilized perlite, and an impermeable turnover box (40 cm×25 cm×15 cm) was placed unde...
Embodiment 2
[0039] Effect of different strains on infection rate.
[0040] Using the device of the present invention to synthesize ectomycorrhizae, the infection rates of different bacterial strains were compared, see Table 2 for details.
[0041] Table 2
[0042] strain type normal method The device of the present invention colored bean puffball 55% 72% Amethyst wax mushroom 59% 84% Delicious Porcini Mushrooms 48% 69% Rhizopus flavonoides 43% 57%
[0043] It can be seen that the use of the mycorrhizal synthesis device of the present invention to cultivate mycorrhizal seedlings is superior to conventional fungus through the infection tests of four different bacteria such as colored bean puffball, amethyst wax mushroom, delicious boletus, and rhizophyllum. The method of mixing soil with blocks, and the time is shortened, and the cost is relatively reduced.
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