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Method of utilizing rice bran hydrolysate fermentation to prepare bacterial cellulose

A technology of bacterial cellulose and bacterial cellulose membrane, which is applied in the field of bacterial cellulose preparation, can solve the problems of high raw material production cost, complex process, and environmental pollution, and achieve the effects of environmental protection, simple preparation process, and abundant sources

Pending Publication Date: 2019-09-06
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the main bottlenecks affecting the industrial production of bacterial cellulose is the high production cost of raw materials
It has been reported that the use of lignocellulose, straw and other biomass raw material hydrolyzate as a carbon source for bacterial cellulose fermentation can reduce the raw material production cost of bacterial cellulose, but in the preparation process of these biomass raw material hydrolyzates, pretreatment The process stage needs to use a large amount of acid or lye, which seriously pollutes the environment; moreover, the hydrolyzate needs to be detoxified, and the process is complicated; in addition, when conventional biomass raw materials are used as fermentation substrates, starch, protein, etc. in the biomass Nutrients are underutilized

Method used

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  • Method of utilizing rice bran hydrolysate fermentation to prepare bacterial cellulose
  • Method of utilizing rice bran hydrolysate fermentation to prepare bacterial cellulose
  • Method of utilizing rice bran hydrolysate fermentation to prepare bacterial cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1): Mechanical pretreatment of rice bran. The rice bran was mechanically pulverized by a high-energy nano-impact mill, the temperature of the working chamber was 10°C, and the pulverization time was 0.5 h to obtain 50-mesh rice bran powder.

[0035] Step 2): Composite enzymatic hydrolysis of rice bran powder. The concentration of rice bran substrate is 20 g / L, gelatinization at 100°C for 30min; add liquefying enzyme (5 U / g), react at 85°C for 1h; cool to 40°C after the reaction, add cellulose to the hydrolyzate Enzyme (10 FPU / g), glucoamylase (100 U / g), 50°C, pH 5, enzymatic hydrolysis for 12 h, after enzymatic hydrolysis, the rice bran hydrolyzate was obtained by suction filtration.

[0036] Step 3): Controlled fermentation of rice bran hydrolyzate. Using rice bran hydrolyzate as raw material, fermentation media containing 1%, 2%, 3%, 4% and 5% glucose were prepared, respectively, containing 1 g / L yeast powder, 1 g / L peptone, and 2 mL / L absolute ethanol. L; Ino...

Embodiment 2

[0041] Step 1): Mechanical pretreatment of rice bran. The rice bran was mechanically pulverized by a high-energy nano-impact mill, the temperature of the working chamber was 25 °C, and the pulverization time was 2 h to obtain 100-mesh rice bran powder.

[0042]Step 2): Composite enzymatic hydrolysis of rice bran powder. The concentration of rice bran substrate was 30 g / L, gelatinized at 100°C for 40 minutes; liquefied enzyme (8 U / g) was added, and reacted at 90°C for 2 hours; after the reaction was completed, it was cooled to 50°C, and cellulose was added to the hydrolyzate Enzyme (15 FPU / g), glucoamylase (200 U / g), 50°C, pH 5, enzymatic hydrolysis for 12 h, after enzymatic hydrolysis, the rice bran hydrolyzate was obtained by suction filtration.

[0043] Step 3): Controlled fermentation of rice bran hydrolyzate. Using rice bran hydrolyzate as raw material, fermentation media containing 4% glucose, 2 g / L yeast powder, 3 g / L peptone, and 3 mL / L absolute ethanol were prepared ...

Embodiment 3

[0048] Step 1): Mechanical pretreatment of rice bran. The rice bran was mechanically pulverized by a high-energy nano-impact mill, the temperature of the working chamber was 10°C, and the pulverization time was 0.5 h to obtain 50-mesh rice bran powder.

[0049] Step 2): Composite enzymatic hydrolysis of rice bran powder. The concentration of rice bran substrate is 30 g / L, gelatinization at 100°C for 60min; add liquefying enzyme (10 U / g), react at 85°C for 1h; cool to 60°C after the reaction, add cellulose to the hydrolyzate Enzyme (20 FPU / g), glucoamylase (300 U / g), 50°C, pH 5, enzymatic hydrolysis for 24 h, after enzymatic hydrolysis, the rice bran hydrolyzate was obtained by suction filtration.

[0050] Step 3): Controlled fermentation of rice bran hydrolyzate. Using rice bran hydrolyzate as raw material, fermentation media containing 4% glucose, 5 g / L yeast powder, 5 g / L peptone, and 4 mL / L absolute ethanol were prepared respectively; 2% to 10% of different inoculum volum...

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Abstract

The invention discloses a method of utilizing rice bran hydrolysate fermentation to prepare bacterial cellulose and belongs to the technical field of biology. The method includes: 1), mechanically pretreating rice bran; 2), subjecting rice bran powder to compound enzymolysis; 3), subjecting rice bran hydrolysate to controllable fermentation; 4), sterilizing a bacterial cellulose membrane; 5), purifying a sterile bacterial cellulose membrane. Raw materials of the method are from rice processing byproducts, so that cost is low, process is simple, high bacterial cellulose generating ability is realized while product high crystallinity is ensured, production cost of bacterial cellulose can be lowered effectively, agricultural biomass waste resources can be utilized fully, high-value and comprehensive utilization of the agricultural biomass waste resources is promoted, and ecological environment is protected.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing bacterial cellulose by fermenting rice bran hydrolyzate. Background technique [0002] Rice is one of the main food crops in China. Although rice bran, a by-product of its processing, only accounts for 6% to 8% of the quality of rice, it accounts for about 60% of the nutritional content of rice. Rice bran China's annual output of rice bran is more than 14 million tons, ranking first in the world. The starch content in rice bran is 28% to 42%, the protein content is 10% to 20%, the fat content is 11% to 25%, the dietary fiber content is 23% to 30%, the ash content is 8% to 12%, and the moisture content is 7% to 15%. %, and rich in vitamins and minerals, the development potential is huge, but the deep processing is insufficient at present, except for a few rice bran raw materials used to extract rice bran oil and rice bran protein, which are generall...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/20C12P19/14C12P19/02C12R1/01
CPCC12P19/02C12P19/04C12P19/14C12P19/20
Inventor 沙如意毛建卫崔艳丽方晟葛青毛旸晨
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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