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T1-T2 Double nuclear magnetic resonance imaging contrast agent and preparation method and application thereof

A nuclear magnetic resonance imaging, T1-T2 technology, applied in the direction of pharmaceutical formulations, preparations for in vivo tests, emulsion delivery, etc., to achieve good imaging effects, uniform particle size, and rapid solid-liquid separation

Active Publication Date: 2019-08-23
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has mild reaction conditions, simple operation process, and is easy to separate and purify; the Fe prepared by the present invention 3 o 4 Magnetic nanoparticles have good colloidal stability, biocompatibility and tumor targeting, but they can only be used for T 2 weighted imaging

Method used

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  • T1-T2 Double nuclear magnetic resonance imaging contrast agent and preparation method and application thereof
  • T1-T2 Double nuclear magnetic resonance imaging contrast agent and preparation method and application thereof
  • T1-T2 Double nuclear magnetic resonance imaging contrast agent and preparation method and application thereof

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Embodiment 1

[0071] This embodiment provides a T 1 -T 2 A method for preparing a dual-nuclear magnetic resonance imaging contrast agent, the method comprising the steps of:

[0072] (1) Dissolve 1 g of carboxymethyl chitosan in 50 mL of deionized water to obtain a carboxymethyl chitosan solution with a concentration of 20 g / L, place it in a three-necked flask, feed nitrogen gas for 30 minutes, and heat the oil bath to 102 °C Obtain reaction system A, wherein the molecular formula of carboxymethyl chitosan is:

[0073]

[0074] Wherein, n is any integer in 100-500;

[0075] (2) Take FeCl3 ·6H 2 O and FeCl 2 4H 2 O is dissolved in hydrochloric acid solution with a hydrogen ion concentration of 1mol / L, FeCl 3 ·6H 2 O and FeCl 2 4H 2 The molar ratio of O is 2:1.5;

[0076] (3) Inject solution B into reaction system A, slowly add ammonia water with a mass fraction of 25 wt.% to adjust the pH to 10, and reflux at 100° C. for 100 minutes to obtain reaction system C;

[0077] (4) Coo...

Embodiment 2

[0091] This embodiment provides a T 1 -T 2 A method for preparing a dual-nuclear magnetic resonance imaging contrast agent, the method comprising the steps of:

[0092] (1) Dissolve 1g of carboxymethyl chitosan in deionized water to obtain a carboxymethyl chitosan solution with a concentration of 15g / L, place it in a three-necked flask, feed nitrogen gas for 40min, and heat the oil bath to 110°C to obtain Reaction system A, wherein the molecular formula of carboxymethyl chitosan is:

[0093]

[0094] Wherein, n is any integer in 100-500;

[0095] (2) Take FeCl 3 ·6H 2 O and FeCl 2 4H 2 O is dissolved in a hydrochloric acid solution with a hydrogen ion concentration of 1.5 mol / L, FeCl 3 ·6H 2 O and FeCl 2 4H 2 The molar ratio of O is 2:1.2.

[0096] (3) Inject solution B into reaction system A, slowly add ammonia water with a mass fraction of 27 wt.% to adjust the pH to 11, and reflux at 110° C. for 90 minutes to obtain reaction system C;

[0097] (4) Cool the re...

Embodiment 3

[0103] This embodiment provides a T 1 -T 2 A method for preparing a dual-nuclear magnetic resonance imaging contrast agent, the method comprising the steps of:

[0104] (1) Dissolve carboxymethyl chitosan in deionized water to obtain a carboxymethyl chitosan solution with a concentration of 18g / L, place it in a three-necked flask, feed nitrogen gas for 40 minutes, and heat the oil bath to 90°C to react System A, wherein the molecular formula of carboxymethyl chitosan is:

[0105]

[0106] Wherein, n is any integer in 100-500;

[0107] (2) Take FeCl 3 ·6H 2 O and FeCl 2 4H 2 O dissolved in a sulfuric acid solution with a hydrogen ion concentration of 2 mol / L, FeCl 3 ·6H 2 O and FeCl 2 4H 2 The molar ratio of O is 2:1.8.

[0108] (3) Inject solution B into reaction system A, slowly add ammonia water with a mass fraction of 28 wt.% to adjust the pH to 11, and reflux at 90° C. for 80 minutes to obtain reaction system C;

[0109] (4) Cool the reaction system C to 25°...

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Abstract

The invention belongs to the technical field of nuclear magnetic resonance, and relates to a T1-T2 double nuclear magnetic resonance imaging contrast agent and a preparation method and application thereof. The contrast agent comprises ferroferric oxide magnetic nanoparticles wrapped by carboxymethyl chitosan, the size of the magnetic nanoparticles is 5-25 nanometers, and the hydrate particle sizeis 20-200 nanometers; the contrast agent has superparamagnetism, a prominent T1 and T2 relaxation enhancement effect and a good long circulation effect, after function exertion is completed, the contrast agent can be metabolized by the body, the preparation method is simple, the mild conditions are achieved, and the cost is low; the contrast agent can be freeze-dried, when the contrast agent is preserved in a solid state, the preservation period is long, and when the contrast agent is redissolved in deionized water, the characters of the contrast agent are hardly changed.

Description

technical field [0001] The invention belongs to the technical field of nuclear magnetic resonance, relates to a contrast agent and its preparation method and application, in particular to a T 1 -T 2 Dual-nuclear magnetic resonance imaging contrast agent and its preparation method and application. Background technique [0002] According to the type of imaging enhancement, the currently used MRI contrast agents are divided into two categories: positive contrast agents and negative contrast agents. They respectively enhance the 1 / T of the local area 1 or 1 / T 2 Relaxation efficiency to achieve the effect of image signal enhancement to brighten or darken the picture. The commonly used negative contrast agent is iron, but many of them are unstable in nature due to large particle size, or can only be stable in the organic phase due to the limitation of the preparation method, which makes the application limited. [0003] In addition, most contrast agents only have one contrast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/18
CPCA61K49/126A61K49/1863
Inventor 陈春英刘涛周会鸽
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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