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Magnetic graphene oxide composite material immobilized horse radish peroxidase as well as preparation method and application thereof

A technology of horseradish peroxidase and composite materials, which is applied in biochemical equipment and methods, oxidoreductases, chemical instruments and methods, etc., can solve the problems of short catalytic life and enzyme inactivation of horseradish peroxidase. , to achieve the effect of improving the ability of reuse, improving activity and facilitating recovery

Active Publication Date: 2017-12-08
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

One of the significant disadvantages of this method is the relatively short catalytic lifetime of the horseradish peroxidase involved in the reaction due to the inactivation of the enzyme due to polymerization

Method used

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  • Magnetic graphene oxide composite material immobilized horse radish peroxidase as well as preparation method and application thereof
  • Magnetic graphene oxide composite material immobilized horse radish peroxidase as well as preparation method and application thereof
  • Magnetic graphene oxide composite material immobilized horse radish peroxidase as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Preparation of novel magnetic graphene oxide composite material

[0059] (1) Synthesis of surface carboxylated graphene oxide GO-COOH

[0060] Graphene oxide (GO) was synthesized by the Hummers method. First, take 0.3 g graphite powder, 0.7 g sodium nitrate in 23 mL 12mol L -1 The concentrated sulfuric acid solution was stirred in an ice bath for 15 minutes. After slowly adding 2.0 g of potassium permanganate, it was transferred to a water bath at 40° C. and stirred for 90 minutes. Add 25 mL of deionized water to the reaction system for dilution and continue stirring for 10 minutes. Add 4 mL of hydrogen peroxide, the system becomes a golden yellow solution, let it stand for 24 hours, pour out the supernatant, and filter with suction to obtain the precipitate, which is washed repeatedly with 5% hydrochloric acid until it is washed with BaCl 2 Detect that there is no precipitation in the supernatant, then wash with deionized water, and centrifuge until ...

Embodiment 2

[0065] Embodiment 2: Preparation of novel magnetic graphene oxide composite material

[0066] (1) Synthesis of surface carboxylated graphene oxide GO-COOH

[0067] Graphene oxide (GO) was synthesized by the Hummers method. First, take 0.7 g graphite powder, 0.3 g sodium nitrate in 23 ml 12mol L -1 The concentrated sulfuric acid solution was stirred in an ice bath for 15 minutes. After slowly adding 6.0 g of potassium permanganate, it was transferred to a water bath at 40° C. and stirred for 90 minutes. Add 75 ml of deionized water to the reaction system for dilution and continue stirring for 10 minutes. Add 8 ml of hydrogen peroxide, the system becomes a golden yellow solution, let it stand for 24 hours, pour out the supernatant, and filter with suction to obtain the precipitate, which is washed repeatedly with 5% hydrochloric acid until it is washed with BaCl 2Detect that there is no precipitation in the supernatant, then wash with deionized water, and centrifuge until t...

Embodiment 3

[0072] Embodiment 3: Preparation of novel magnetic graphene oxide composite material

[0073] (1) Synthesis of surface carboxylated graphene oxide GO-COOH

[0074] Graphene oxide (GO) was synthesized by the Hummers method. First, take 0.5 g graphite powder, 0.5 g sodium nitrate in 23 ml 12mol L -1 The concentrated sulfuric acid solution was stirred in an ice bath for 15 minutes. After slowly adding 4.0 g of potassium permanganate, it was transferred to a water bath at 40° C. and stirred for 90 minutes. Add 50 ml of deionized water to the reaction system for dilution and continue stirring for 10 minutes. Add 6 ml of hydrogen peroxide, the system becomes a golden yellow solution, let it stand for 24 hours, pour out the supernatant, and filter with suction to obtain the precipitate, which is washed repeatedly with 5% hydrochloric acid until it is washed with BaCl 2 Detect that there is no precipitation in the supernatant, then wash with deionized water, and centrifuge until ...

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Abstract

The invention relates to magnetic graphene oxide composite material immobilized horse radish peroxidase as well as a preparation method and application thereof, belonging to the technical field of inorganic materials and analysis. The preparation method comprises the steps of firstly preparing a magnetic polymer microsphere containing 6-arm polyethylene glycol amide (6-arm-PEG-NH2) from magnetic graphene oxide GO-Fe3O4 and 6-arm-PEG-NH2, immobilizing horse radish peroxidase, and degrading pollutant phenol by virtue of immobilized horse radish peroxidase. The synthetic process of the material is reasonable in design, and the carrier material is coupled with zymoid catalytic activity of graphene oxide and high density amino functional groups on the surface of a multi-arm polymer 6-arm-PEG-NH2, so that the activity and reutilization capacity of immobilized horse radish peroxidase are effectively improved; furthermore, the degradation velocity of prepared immobilized horse radish peroxidase is obviously higher than the degradation velocities of free enzyme and a carrier material.

Description

technical field [0001] The invention belongs to the technical field of inorganic materials and analysis, and relates to a magnetic graphene oxide composite material immobilized horseradish peroxidase and a preparation method and application thereof. Background technique [0002] Horseradish peroxidase (HRP) is a promising biocatalyst that has been reported for the removal of phenolic compounds and aromatic amines in solution and the decolorization of textile wastewater. HRP catalyzes the oxidation of phenols to phenoxy groups in the presence of hydrogen peroxide. The free radicals generated form insoluble polymers, which can be isolated by simple filtration or decantation. One of the significant disadvantages of this method is the relatively short catalytic lifetime of the horseradish peroxidase involved in the reaction due to the inactivation of the enzyme by polymerization. In order to solve this problem, Chen et al. (Cheng J, Ming Y S, Zuo P. Horseradishperoxidase immob...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/08C02F3/34C02F101/34
CPCC02F3/34C02F2101/345C12N9/0065C12N11/08C12N11/14C12Y111/01007
Inventor 顾磊王赟韩娟李程王蕾唐旭倪良
Owner JIANGSU UNIV
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