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MoS2 QDs fluorescent probe, and synthesis method and applications thereof

A technology of a fluorescent probe and a synthesis method, which is applied in the fields of transition metal sulfide quantum dot bio-fluorescent probes and bio-fluorescent probes, can solve the problems of easy oxidation, complicated production process of fluorescent probes, and low quantum yield of fluorescent probes. problems, to achieve the effect of reducing biological toxicity, improving antioxidant and photobleaching properties

Active Publication Date: 2019-08-16
CHAOHU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quantum yield of these fluorescent probes is often not high, and the production process of fluorescent probes is cumbersome
The surface of graphene quantum dots contains a large number of C=C double bonds, which are easy to oxidize, and its oxidation resistance and photobleaching performance need to be improved

Method used

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  • MoS2 QDs fluorescent probe, and synthesis method and applications thereof
  • MoS2 QDs fluorescent probe, and synthesis method and applications thereof
  • MoS2 QDs fluorescent probe, and synthesis method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] (1)MoS 2 Preparation of quantum dot powder

[0061] 1) Dissolve 0.225 g and 0.13 g of cystamine dihydrochloride and ammonium tetrathiomolybdate in 36 mL of deionized water, and adjust the pH value of the mixed solution to 7 with ammonia water or hydrochloric acid.

[0062] 2) After the above mixed solution was sonicated for 25 minutes, it was transferred into a 50 mL stainless steel reaction kettle with a polytetrafluoroethylene liner, heated at 200° C., and reacted for 16 hours.

[0063] 3) After the reaction is completed, the reactor is left to stand for 5 minutes. Take the supernatant suspension, and use a high-speed centrifuge to centrifuge at a speed of 10,000 n / min for 20 minutes at a high speed. After centrifugation, the supernatant is filtered with a filter membrane with a pore size of 0.22 μm to obtain a solution.

[0064] 4) Dialyze the solution obtained in the above steps with a dialysis bag with a molecular weight cut-off of 1000, put the dialyzed solution...

Embodiment 2

[0081] (1)MoS 2 Preparation of quantum dot powder

[0082] 1) Dissolve 0.338 g and 0.13 g of cystamine dihydrochloride and ammonium tetrathiomolybdate in 36 mL of deionized water, respectively. Adjust the pH value of the mixed solution to 8 with ammonia water or hydrochloric acid.

[0083] 2) After the above mixed solution was sonicated for 20 minutes, it was transferred into a 50 mL stainless steel reaction kettle with a polytetrafluoroethylene liner, heated at 210° C., and reacted for 14 hours.

[0084] 3) After the reaction is finished, the reactor is left to stand for 10 minutes. Take the supernatant suspension and use a high-speed centrifuge to centrifuge at a speed of 10,000 n / min for 20 minutes. After centrifugation, the supernatant is filtered with a filter membrane with a pore size of 0.22 μm to obtain a solution.

[0085] 4) Dialyze the solution obtained in the above steps with a dialysis bag with a molecular weight cut-off of 1000, put the dialyzed solution in a ...

Embodiment 3

[0093] (1)MoS 2 Preparation of quantum dot powder

[0094] 1) Dissolve 0.45 g and 0.13 g of cystamine dihydrochloride and ammonium tetrathiomolybdate in 36 mL of deionized water, and adjust the pH value of the mixed solution to 9 with ammonia water or hydrochloric acid.

[0095] 2) After the above mixed solution was sonicated for 15 minutes, it was transferred into a 50 mL stainless steel reaction kettle with a polytetrafluoroethylene liner, heated at 220° C., and reacted for 12 hours.

[0096] 3) After the reaction is completed, the reactor is left to stand for 15 minutes. Take the supernatant suspension and use a high-speed centrifuge to centrifuge at a speed of 10,000 n / min for 20 minutes. After centrifugation, the supernatant is filtered with a filter membrane with a pore size of 0.22 μm to obtain a solution.

[0097] 4) Dialyze the solution obtained in the above steps with a dialysis bag with a molecular weight cut-off of 1000, put the dialyzed solution in a vacuum dryi...

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Abstract

The invention discloses a synthesis method of a MoS2 QDs fluorescent probe. The synthesis method comprises following steps: (1) dissolving cystamine dihydrochloride and ammonium tetrathiomolybdate into deionized water to obtain a mixed solution with a pH value of 7 to 11; (2) processing the mixed solution by ultrasonic waves, and then heating the mixed solution to carry out reactions; (3) after reactions, making the system to stand still, and subjecting the upper layer turbid liquid to centrifugation; (4) after centrifugation, filtering the upper layer supernate to obtain a filtered solution;(5) subjecting the filtered solution to dialysis, and carrying out vacuum drying to obtain MoS2 QDs powder; (6) preparing a water solution of MoS2 QDs from the MoS2 QDs powder obtained in the step (5), wherein the MoS2 QDs powder water solution is used as a MoS2 QDs fluorescent probe for detecting heme. The invention also discloses a MoS2 QDs fluorescent probe and applications thereof. The provided MoS2 QDs fluorescent probe has the advantages of good biocompatibility, high stability, high fluorescence quantum yield, simple synthesis conditions, and low price. When the fluorescent probe is used to detect heme, the detection process is simple, and the sensitivity is high.

Description

technical field [0001] The invention relates to the technical field of biological fluorescent probes, in particular to the technical field of transition metal sulfide quantum dot biological fluorescent probes. Background technique [0002] High or low hemoglobin in human blood (hereinafter referred to as hemoglobin) can cause various diseases and even endanger life. It is of great significance to detect heme in human body and functional food. Organic dyes and fluorescent proteins are used as fluorescent probes to test heme, but their fluorescence intensity is low and the emission spectrum is wide. Traditional II-VI, III-V quantum dot fluorescent probes are used, which contain heavy metals such as Cd and Pb, which are highly toxic to organisms. Materials such as carbon quantum dots and graphene quantum dots can be modified by coupling with antibodies or polypeptides to obtain better biocompatibility. However, the quantum yield of these fluorescent probes is often not high,...

Claims

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Application Information

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IPC IPC(8): C09K11/68B82Y20/00B82Y40/00G01N21/64
CPCC09K11/681B82Y20/00B82Y40/00G01N21/6428G01N21/6486G01N2021/6432
Inventor 吴凤义王新运李川高玉荣李明玲高立王志海邵娟娟武梦婷赵孝郑陈晨吴梦晴吴凤彬
Owner CHAOHU UNIV
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