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Preparation method of swine pseudorabies virus high-immune serum

A porcine pseudorabies virus and hyperimmune serum technology, applied in the field of biological products, can solve the problems of weak antibody ability, less blood collected from rabbits, and inaccurate description of whether the test results are correct, etc., to achieve a wide range of applications and improve the effect of yield.

Pending Publication Date: 2019-08-09
成都史纪生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most porcine pseudorabies hyperimmune serum uses heterologous animals such as rabbits and sheep, but there are factors such as the small amount of blood collected from rabbits, lack of detection methods for foreign virus in sheep, and insufficient accuracy to explain whether the test results are correct or not.
However, the neutralizing antibodies of the existing swine-derived pseudorabies hyperimmune serum are generally weak, and cannot be applied to the detection of high-titer finished vaccines today.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 porcine pseudorabies virus vaccine preparation

[0034] 1. Preparation of Immunopotentiator

[0035] Add interferon α, interferon β, astragalus polysaccharide and macrophage colony-stimulating factor in 0.9% sodium chloride buffer solution (pH7.2) and prepare water phase, make every milliliter of water phase contain 50mg interferon α, 50mg interferon Prime β, 200mg astragalus polysaccharide and 10mg macrophage colony-stimulating factor were mixed with Tween-60 at a volume ratio of 9:1, emulsified with a homogenizer at 20-200Hz for 3-10min at 25°C, and prepared into immune enhancer.

[0036] 2. Preparation of Viral Inactivator

[0037] For the preparation of BEI (diethyleneimine) inactivator, weigh 0.1 g of BEA and 0.1 g of NaOH, dissolve them in 5 ml of deionized water, and inactivate them in a 37°C water bath for 1 hour after mixing. This solution should be used immediately after preparation.

[0038] 3. Preparation of inactivated virus

[0039] This ...

Embodiment 2

[0044] Embodiment 2 porcine pseudorabies virus vaccine preparation

[0045] 1. Preparation of Immunopotentiator

[0046] Add interferon α, interferon β, astragalus polysaccharide and macrophage colony-stimulating factor in 0.9% sodium chloride buffer solution (pH7.2) and prepare water phase, make every milliliter of water phase contain 0.1mg interferon α, 0.1 mg interferon β, 0.1 mg astragalus polysaccharide and 0.1 mg macrophage colony-stimulating factor, then mix with Tween-60 at a volume ratio of 10:1, and emulsify with a homogenizer at 20-200 Hz for 3-10 min at 25 °C , prepared as an immune enhancer. 2. Preparation of Viral Inactivator

[0047] With embodiment 1.

[0048] 3. Preparation of inactivated virus

[0049] With embodiment 1.

[0050] 4. Preparation of Inactivated Vaccine

[0051] With embodiment 1.

Embodiment 3

[0052] Embodiment 3 porcine pseudorabies virus vaccine preparation

[0053] 1. Preparation of Immunopotentiator

[0054]Add interferon α, interferon β, astragalus polysaccharide and macrophage colony-stimulating factor in 0.9% sodium chloride buffer solution (pH7.2) and prepare water phase, make every milliliter of water phase contain 100mg interferon α, 100mg interferon Primer β, 200mg astragalus polysaccharide and 100mg macrophage colony-stimulating factor were then mixed with Tween-60 at a volume ratio of 8:1, emulsified with a homogenizer at 20-200Hz for 3-10min at 25°C, and prepared into immune enhancer. 2. Preparation of Viral Inactivator

[0055] With embodiment 1.

[0056] 3. Preparation of inactivated virus

[0057] With embodiment 1.

[0058] 4. Preparation of Inactivated Vaccine

[0059] With embodiment 1.

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Abstract

The invention relates to a preparation method of a swine pseudorabies virus high-immune serum. The serum is obtained by taking pigs as immunization objects, conducting primary basic immunization and secondary enhanced immunization by using an inactivated vaccine, primarily counteracting toxic substances by using a porcine pseudorabies virus, and then executing collection; the inactivated vaccine contains an immunopotentiator of interferon alpha, interferon beta, astragalus polysaccharide and a macrophage colony stimulating factor. The swine pseudorabies virus high-immune serum can overcome theshortcomings of poor neutralization capacity of high-immune serum antibodies derived from pigs, and can be widely applied to strains JS-2012, Bartha-k61, HB-98, HB2000 and / or JS-A1 of pseudorabies viruses and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a method for preparing porcine pseudorabies virus hyperimmune serum. Background technique [0002] Hyperimmune serum is made by using a certain vaccine (bacteria) to repeatedly immunize a certain animal according to a certain procedure, and after the antibody titer reaches a certain level, the immunized animal is slaughtered and the whole blood is collected, and then it is made by special treatment. of a biological product. Hyperimmune serum is mainly used for exogenous virus detection of biological products. [0003] Porcine pseudorabies virus hyperimmune serum is mainly used for exogenous virus detection of veterinary biological products. With the improvement of the manufacturing process of porcine pseudorabies live vaccines, the virus potency of finished vaccines from various manufacturers has increased from 10 to 10. 5.0 TCID 50 / head count increased to 10 6....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/06C07K1/34
CPCC07K16/085C07K16/06
Inventor 邢刚王洁清岳丰雄方芳杨勇黄杰袁雪林魏胜男李晏齐王开林徐祥兰
Owner 成都史纪生物制药有限公司
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