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Application of SOX4 inhibitor and medicine containing SOX4 inhibitor

A technology of SOX4, 1.SOX4, applied in the field of inhibitor drugs, can solve problems that have not been reported yet

Inactive Publication Date: 2019-08-02
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of SOX4 in CNS microglia has not been reported

Method used

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  • Application of SOX4 inhibitor and medicine containing SOX4 inhibitor
  • Application of SOX4 inhibitor and medicine containing SOX4 inhibitor
  • Application of SOX4 inhibitor and medicine containing SOX4 inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1 animal and grouping

[0074] 100 SPF-grade healthy newborn 3-day-old male SD (Sprague Dawley) rats were provided by the Experimental Animal Center of Kunming Medical University. They were housed in separate cages at a room temperature of 22±2°C, free to eat and drink, and there were no differences in exposure factors among the groups. The treatment of animals complied with the ethical requirements of experimental animals.

[0075] Rats were injected intraperitoneally with LPS (1mg / kg) to construct an inflammation model. The blank group was given equal doses of normal saline, and treated at 2, 4, and 8 hours respectively, and divided into 2h group, 4h group, 8h group and blank group. .

[0076] BV-2 and primary microglial cells were cultured in vitro, stimulated with LPS (1 μg / ml) for 1, 3, and 6 hours, respectively, to build an inflammation model, which was divided into 1 hour group, 3 hour group and 6 hour group, simulating neurodegeneration and Inflamma...

Embodiment 2

[0077] Example 2 Immunohistochemical fluorescent staining

[0078] At the corresponding time points, the rats in each group were punctured through the apex of the heart with a syringe, slowly pushed into normal saline perfusion, and then given 4% paraformaldehyde buffer (PFA). Dehydrate in sucrose solution until it sinks to the bottom. Coronal slices were cut with a frozen microtome (Leica, CM1950) with a thickness of 10 μm. One slice was randomly selected from each group, rinsed with PBST, and blocked with 10% goat serum for 2 hours at room temperature. Incubate with primary antibody for 2 hours, rinse with PBST (the following steps are protected from light), add immunofluorescent secondary antibody for incubation for 2 hours, rinse with PBST, and mount with DAPI-containing mounting solution. For the negative control, PBS was used instead of the primary antibody, and the rest of the steps were the same. Observation with a Nikon upright fluorescence microscope, and filming u...

Embodiment 3

[0082] Example 3 Western blot

[0083] BCA protein quantification kit for protein quantification, SDS-polyacrylamide gel electrophoresis, semi-dry transfer to PVDF membrane, block in 5% skimmed milk prepared in TBST for 2 hours at room temperature, add primary antibody, and incubate overnight at 4°C; TBST solution After rinsing, IgG secondary antibody labeled with horseradish enzyme peroxidase was added, shaken and incubated at room temperature for 90 minutes, rinsed with TBST, reacted with immunoblotting chemiluminescent reagent (ECL, millipore) for 5 minutes, film developed, imaged, and scanned. The optical density (OD) analysis of the bands was performed using the Image J image grayscale analysis system. Repeat 3 times.

[0084] The Western blot results of the expression of SOX4 in microglial cells in the corpus callosum of SD rats are as follows: Figure 4A As shown, the grayscale image is shown as Figure 4BAs shown, it can be seen from the figure that the expression o...

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Abstract

The invention provides application of an SOX4 inhibitor and medicine containing the SOX4 inhibitor, and relates to the technical field of biological medicine engineering. The invention provides the application of the SOX4 inhibitor to preparation of a product for inhibiting microglial cell mediated inflammatory reaction; by inhibiting the expression of SOX4 in microglial cells, the SOX4 inhibitorcan effectively reduce the expression of proinflammatory factors in inflammation model microglial cells; A result that by inhibiting the SOX4, the inflammatory reaction of the microglial cells in theinflammation pathological process of neurodegenerative diseases and nervous system degenerative diseases can be reduced is shown. The result provides novel action target spots and mechanisms for clinically treating the neurodegenerative diseases and the nervous system degenerative diseases; the theoretical support is provided for the development of novel medicine.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to the application of a SOX4 inhibitor and the medicine containing the SOX4 inhibitor. Background technique [0002] Microglia are the main immune cells in the central nervous system, and they account for 5% to 20% of the total number of glial cells in the central nervous system. Under normal circumstances, it is in a static state and lacks phagocytic function, but it has swallowing function and certain migration ability. When receiving endogenous or exogenous stimuli, microglial cells are rapidly activated to an activated state, on the one hand, they produce a large number of pro-inflammatory cytokines and neuron-toxic mediators, which mediate chronic inflammatory reactions and apoptosis in the brain, leading to Neuron damage and death; on the other hand, it also plays a role in phagocytosis and secretes nerve growth factor. Therefore, microglia have dual functions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/713A61P29/00A61P25/00
CPCA61K31/713A61P25/00A61P29/00
Inventor 边立功陆地龚霞蓉李瑶孙林杨萍韩雁冰张爱丹高慧青林荣安
Owner KUNMING MEDICAL UNIVERSITY
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