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Preparation for preventing and treating post-harvest mycosis of fruits

A fruit and preparation technology, which is applied in the direction of protecting fruits/vegetables with a coating protective layer, can solve problems such as unsatisfactory bactericidal effect, reduced mold inhibitory effect, and reduced fruit rot rate, achieving significant economic and social benefits , prolong the fresh-keeping time, the effect of prolonging the fresh-keeping time

Inactive Publication Date: 2019-07-23
SHAOXING UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, the bactericidal effect of chitosan oligosaccharide compound preparations on molds and spores is not very satisfactory. For example, Lu Qijie and others found that chitosan oligosaccharide zinc complexes have certain antibacterial effects on Staphylococcus aureus and Escherichia coli (Lu Qijie, Wang Li, Qian Xu, et al. Preparation and antibacterial activity of chitosan oligosaccharide zinc complex [J]. Food Research and Development, 2018); Jiang Lai et al. found that 5g / L and 10g / L shell On the contrary, oligosaccharides promote the growth of Penicillium extensa, but obviously inhibit the secretion of Penicillium extensa toxin, (Jiang spicy, Wang Liting, Chen Haiyan, et al. Effects of oligochitosan on the growth of Penicillium extensa and the secretion of toxin[J ].Journal of Food Science and Technology, 2015(5))
Luo Jin and others found that boric acid can be used for the control of Penicillium digitatum in citrus and achieved certain effects (Luo Jin, Wang Xiaohong, Ye Bishun, et al. Study on the effect of exogenous boric acid on inhibiting postharvest green mold disease Penicillium digitatum in citrus[J] .Journal of Hangzhou Normal University: Natural Science Edition, 2018,17(2):159-165), but these single antibacterial effects are not ideal
However, previous studies on the antibacterial effect of the fungus on Prunus chinensis are rarely reported, although our previous research found that both Shibaogong and Azoxystrobin at an appropriate mass concentration can inhibit the mycelial growth of Penicillium citrinum in Plum. and spore germination (Zhao Ning, Song Yuhui, Chen Li, et al. Isolation, identification and indoor antibacterial test of Penicillium citrinum from peach-shaped plums in Shengzhou[J]. Fujian Agricultural Journal, 2018,33(4):407-412) , but these two chemical fungicides still have the risk of pesticide residues in the postharvest treatment, while 0.20% NaHCO 3 It can inhibit the growth of Penicillium expansum on the medium, but after adopting 0.20% NaHCO 3 After treatment, the fruit rot rate can be reduced in a short period of time, and if the storage time is too long, the rot rate will increase again (He Yingrong, Lin Haiyan, Cao Zongwei, et al. Control effects of different treatments on postharvest pathogens in peach-shaped plums[J]. Tropical Crops , 2017, 38(4):734-739)
But in the fruit treated with NaHCO 3 After treatment, the surface of the fruit is prone to dehydration, causing the skin to be easily damaged to a certain extent, and it can only have a short-term inhibitory effect on mold, and the antibacterial effect on mold will be significantly reduced during long-term storage

Method used

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  • Preparation for preventing and treating post-harvest mycosis of fruits
  • Preparation for preventing and treating post-harvest mycosis of fruits

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: The influence of preparations of different proportions on the growth of mold mycelium and the germination of conidia

[0030] Biological material: silk mold, purchased from Suzhou Beina Chuanglian Biotechnology Co., Ltd. Both Penicillium extensa and Penicillium citrinum were isolated and screened in the laboratory (for related literature, see: He Yingrong, Lin Haiyan, Cao Zongwei, et al. Effects of different treatments on postharvest pathogenic bacteria in peach-shaped plums[J]. Tropical Crops, 2017, 38(4):734-739 and Zhao Ning, Song Yuhui, Chen Li, et al. Isolation, identification and indoor antibacterial test of Penicillium citrinum in peach-shaped plums in Shengzhou[J].Journal of Fujian Agricultural Sciences, 2018,33( 4): 407-412).

[0031]Determination method of mycelial growth inhibition rate: plate culture method (see He Yingrong, 2017) was used to measure the growth inhibition rate of Mycelia spp. 5 cells / mL bacterial liquid; then placed in an inc...

Embodiment 2

[0040] On June 31, 2017, 80% of the mature peach-shaped plums recovered from the planting base were treated with 0.08% boric acid and 1.2% chitosan oligosaccharide compound, and the coated fruits were packed in containers with In the small-hole polyethylene fresh-keeping bag, each bag contains 5kg of fruit, and a total of 200Kg of fruit is processed and divided into 40 bags for storage. Store in an artificial climate box at 26°C and a relative humidity of 85%-90%. At the same time, the fruit washed and soaked with distilled water was used as the control treatment. It was found that the control treatment soaked in distilled water had a disease incidence rate of up to 40.5% when stored for 8 days, while it was treated with 0.08% boric acid and 1.2% chitosan oligosaccharide compound coating, and the incidence rate was only 10% on the 10th day of storage. 9.78%, reaching a significant difference. It shows that the combined treatment can significantly reduce the rot rate of posth...

Embodiment 3

[0042] On July 8, 2017, 90% of the mature peach-shaped plums collected from the planting base were compounded with 0.08% boric acid and 1.0% chitosan oligosaccharides, and the coated fruits were packed in containers with In the small-hole polyethylene fresh-keeping bags, each bag contains 5kg of fruit, and a total of 100Kg of fruit is processed, which is divided into 20 bags for storage. Store in an artificial climate box at 26°C and a relative humidity of 85%-90%. At the same time, the fruit washed and soaked with distilled water was used as the control treatment. It was found that the control treatment soaked in distilled water had a disease incidence rate of 48.75% when stored for 8 days, and was treated with a compound coating of 0.08% boric acid and 1.0% chitosan oligosaccharide, and the incidence rate was only 10% on the 10th day of storage. was 10.72%. It shows that the combined treatment can significantly reduce the rot rate of postharvest fruit, and the effect of pr...

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Abstract

The invention discloses a preparation for preventing and treating post-harvest mycosis of fruits, and belongs to the field of post-harvest fresh-keeping. The preparation consists of 0.08-0.15% boric acid and 0.8-1.2% chitosan oligosaccharide. According to the invention, the boric acid and chitosan oligosaccharide composite preparation has a better bacteriostatic effect on penicillium expansum, byssochlamys and penicillium citrinum. Compared with single treatment of the chitosan oligosaccharide or the boric acid, the chitosan oligosaccharide and the boric acid can generate a synergistic effect,can effectively inhibit the growth of spores and hyphae, and have a better bactericidal effect. In addition, the preparation of the invention obviously improves the hardness of fruits, maintains highcontent of soluble solid matters in the fruits, obviously reduces the incidence rate of the fruits, reduces the occurrence of mildew, improves the fresh-keeping effect, and prolongs the preservationtime.

Description

technical field [0001] The invention relates to a preparation for preventing and treating post-harvest mold diseases of fruits, which belongs to the field of post-harvest preservation. Background technique [0002] Shengzhou peach-shaped plum is a famous and high-quality fruit in Shengzhou, Zhejiang, and it is also an important economic pillar of the producing area. Peach-shaped plum fruit is heart-shaped, with an average fruit weight of 41.5g. When ripe, the surface of the fruit is red, the flesh is purple-red, sweet and fragrant, and the edible rate is 97.8%. It is very popular among consumers. The fruit matures in mid-July every year. During the high temperature and rainy season, peach-shaped plums are respiration-type fruits. After harvesting, the respiration is strong, and the skin cracks after softening quickly. Because the fruit is rich in sugar and other nutrients, it is susceptible to various molds, which accelerates the fruit’s rot and makes it difficult to keep fr...

Claims

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Application Information

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IPC IPC(8): A23B7/16
CPCA23B7/16
Inventor 莫亿伟叶建平郭天荣王海
Owner SHAOXING UNIVERSITY
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