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A kind of method extracting dehydroepiandrosterone from sweet potato

A dehydroepiandrosterone, sweet potato technology, applied in the direction of steroids, organic chemistry, etc., can solve the problems of long time, low efficiency, low extraction rate of DHEA from sweet potato, etc., and achieve stable product quality, short pretreatment time, and process Conditional effects

Active Publication Date: 2021-11-05
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In sweet potatoes, DHEA mainly exists in the plant cell wall in the form of diosgenin combined with cellulose. The common method for extracting DHEA from sweet potatoes is acid hydrolysis, but because dioscin is closely combined with cellulose in sweet potatoes, traditional acid The hydrolysis method cannot completely separate the bound diosgenin from the cellulose, so the extraction rate of sweet potato DHEA obtained by the traditional method is low and takes a long time and low efficiency
For this reason, Yao Juying et al. carried out natural fermentation treatment on sweet potatoes before hydrolysis, which is conducive to improving the product yield, and the yield can reach 5 / 10,000. However, this method takes a long time, and only fermentation takes two days. The process is uncontrollable, resulting in unstable product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting dehydroepiandrosterone from sweet potatoes, the steps are as follows:

[0028] 1) Pre-conversion treatment:

[0029] Wash the fresh sweet potatoes to remove impurities, make a pulp, add 20% by weight of the treatment liquid to the sweet potato pulp, stir at a constant temperature at 8°C for 4 hours, then wash the sweet potato pulp with water to neutrality, filter and then add 1.6 times the weight of the sweet potato pulp to the sweet potato pulp Grind with water for 30 minutes with a colloid mill, centrifuge, and place the resulting precipitate in a water bath at 60°C for 12 hours. The main function of the water bath is to enzymatically convert a part of furostanol saponin into spirosterol saponin, and the latter is more easily converted into diosgenin. Thereby further improving the yield of saponin.

[0030] The treatment liquid is NaOH, urea, CaCl 2 Mixed solution, NaOH, urea, CaCl in the mixed solution 2 The weight concentrations are 8%, 8%...

Embodiment 2

[0038] A method for extracting dehydroepiandrosterone from sweet potatoes, the steps are as follows:

[0039] 1) Clean and remove impurities from fresh sweet potatoes, make a pulp, add 15% by weight of the treatment solution to the sweet potato pulp, stir at a constant temperature at 0°C for 5 hours, then wash the sweet potato pulp with water until neutral, filter and then add 2 times weight of water and grind with a colloid mill for 60 min, centrifuge, and place the resulting precipitate in a water bath at 80°C for 8 h.

[0040] The treatment liquid is lithium hydroxide, urea, ZnCl 2 Mixed solution, lithium hydroxide, urea, ZnCl in the mixed solution 2 The weight concentrations are 5%, 10%, and 5%, respectively.

[0041] 2) Then add 3% by weight of concentrated H to the precipitate 2 SO 4 , hydrolyzed at 80°C for 16h, cooled to room temperature, adjusted the pH to neutral with NaOH solution, centrifuged, and collected the precipitate.

[0042] 3) Add 5 times the weight o...

Embodiment 3

[0045] A method for extracting dehydroepiandrosterone from sweet potatoes, the steps are as follows:

[0046] 1) Clean and remove impurities from fresh sweet potatoes, make a pulp, add 40% by weight of the treatment solution to the sweet potato pulp, stir at a constant temperature at 5°C for 2 hours, then wash the sweet potato pulp with water until it is neutral, filter and then add 1 times to the sweet potato pulp weight of water and grind with a colloid mill for 10 minutes, centrifuge, and place the resulting precipitate in a water bath at 50°C for 24 hours.

[0047] The treatment liquid is potassium hydroxide, urea, MgCl 2 Mixed solution, potassium hydroxide, urea, MgCl in the mixed solution 2 The weight concentrations are 10%, 7%, and 2%, respectively.

[0048] 2) Then add 1% by weight of concentrated H to the precipitate 2 SO 4 , hydrolyzed at 100°C for 8h, cooled to room temperature, adjusted the pH to neutral with NaOH solution, centrifuged, and collected the precip...

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Abstract

The invention discloses a method for extracting dehydroepiandrosterone from sweet potatoes, which comprises the steps of pre-conversion treatment, acid hydrolysis, extraction, purification and the like. The present invention aims at the defect that the combined diosgenin and cellulose cannot be completely separated by traditional acid hydrolysis, resulting in a very low yield of the product. By stirring the sweet potato pulp with a treatment liquid, the cellulose is fully swollen and processed by mechanical grinding. Break the hydrogen bond and part of the glycosidic bond combining sweet potato cellulose and dioscin, promote the separation of dioscin, thereby improving the rate of subsequent acid hydrolysis and the extraction rate of DHEA, and achieve the purpose of rapid and efficient extraction of DHEA in sweet potato.

Description

technical field [0001] The invention relates to a method for extracting dehydroepiandrosterone, in particular to a method for extracting dehydroepiandrosterone from sweet potatoes. Background technique [0002] Dehydroepiandrosterone (DHEA) is one of the most abundant hormones in the human body. It has anti-aging, anti-tumor, anti-obesity, and regulation of human hormones. It is also an intermediate for the manufacture of steroid hormone drugs. There are few natural plants containing DHEA, among which sweet potato is the one with richer content. [0003] In sweet potatoes, DHEA mainly exists in the plant cell wall in the form of diosgenin combined with cellulose. The common method for extracting DHEA from sweet potatoes is acid hydrolysis, but because dioscin is closely combined with cellulose in sweet potatoes, traditional acid The hydrolysis method cannot completely separate the bound diosgenin from the cellulose, so the extraction rate of sweet potato DHEA obtained by th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J1/00
Inventor 王鲁峰邹丹潘思轶徐晓云
Owner HUAZHONG AGRI UNIV
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