Method for extracting astaxanthin through ionic liquid-salt aqueous two-phase system

A two-phase system, ionic liquid technology, applied in the direction of organic chemistry, etc., can solve the problems of high operating pressure of astaxanthin, oxidative decomposition of astaxanthin, easy volatilization of organic solvents, etc., to achieve strong thermochemical stability, biological phase Capacitive and environmentally friendly effects

Active Publication Date: 2019-06-28
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the alkaline extraction method uses a large amount of acid and alkali, it is easy to cause the oxidation and decomposition of astaxanthin
The organic solvent extraction method is to use the molecular structure of astaxanthin to contain hydrophobic groups and the characteristics of being easily soluble in organic solvents to extract astaxanthin from shrimp shells, but the organic solvents are volatile and cause great environmental pollution.
The extraction of astaxanthin by supercritical carbon dioxide extraction has disadvantages such as high operating pressure, high equipment cost, small extraction volume and high cost.

Method used

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  • Method for extracting astaxanthin through ionic liquid-salt aqueous two-phase system
  • Method for extracting astaxanthin through ionic liquid-salt aqueous two-phase system
  • Method for extracting astaxanthin through ionic liquid-salt aqueous two-phase system

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Embodiment 1

[0053] (1) The ionic liquid tributyl octyl phosphine bromide and distilled water are mixed uniformly to prepare an ionic liquid aqueous solution, so that the mass fraction of tributyl octyl phosphine bromide in the ionic liquid aqueous solution is 2%.

[0054] (2) Wash the fresh shrimp shells and freeze-dry them at -50°C for 40 hours, then grind them in a grinder, and pass through a 50-mesh sieve to obtain shrimp shell powder for later use.

[0055] (3) Get the shrimp shell powder that 1g step (1) obtains and the ionic liquid aqueous solution that 35g step (2) obtains and place in the beaker, then adopt magnetic stirrer to stir, set stirring speed to be 1000rpm, stir 60min at room temperature, Then the obtained mixture was centrifuged at a centrifugal speed of 5000rpm for 20min to obtain the supernatant of the ionic liquid aqueous solution from which astaxanthin was extracted and the shrimp shell powder precipitate of astaxanthin, and the supernatant was collected to obtain ast...

Embodiment 2

[0059] (1) The ionic liquid tributyloctylphosphine bromide and distilled water are mixed uniformly to prepare an ionic liquid aqueous solution, so that the mass fraction of tributyloctylphosphine bromide in the ionic liquid aqueous solution is 5%; wherein, the ionic liquid is three Butyloctylphosphine bromide.

[0060] (2) Wash the fresh shrimp shells and freeze-dry them at -55°C for 40 hours, then grind them in a grinder, and pass through a 70-mesh sieve to obtain shrimp shell powder for later use.

[0061] (3) Get 1g of the shrimp shell powder obtained in step (1) and the aqueous ionic liquid solution obtained in 30g of step (2) in a beaker, then use a magnetic stirrer to stir, set the stirring speed to 500rpm, and stir at room temperature for 120min. Then the obtained mixture was centrifuged at a centrifugal speed of 6000rpm for 18min to obtain the supernatant of the ionic liquid aqueous solution from which astaxanthin was extracted and the shrimp shell powder precipitate o...

Embodiment 3

[0065] (1) The ionic liquid tributyl octyl phosphine bromide and distilled water were uniformly mixed to prepare an ionic liquid aqueous solution, so that the mass fraction of tributyl octyl phosphine bromide in the ionic liquid aqueous solution was 8%.

[0066] (2) Wash the fresh shrimp shells and freeze-dry them at -60°C for 30 hours, then grind them in a grinder, and pass them through an 80-mesh sieve to obtain shrimp shell powder for later use.

[0067] (3) Get the shrimp shell powder that 1g step (1) obtains and the ionic liquid aqueous solution that 25g step (2) obtains and place in the beaker, then adopt magnetic stirrer to stir, setting stirring speed is 800rpm, stir 80min at room temperature, Then the obtained mixture was centrifuged at a centrifugal speed of 6000rpm for 18min to obtain the supernatant of the ionic liquid aqueous solution from which astaxanthin was extracted and the shrimp shell powder precipitate of astaxanthin, and the supernatant was collected to ob...

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Abstract

The invention discloses a method for extracting astaxanthin through an ionic liquid-salt aqueous two-phase system. The method comprises the steps that an ionic liquid aqueous solution is prepared, sothat the mass ratio of an ionic liquid is 2-10%; shrimp shell powder and the ionic liquid aqueous solution are mixed and then subjected to magnetic stirring at the normal temperature; a mixture aftermagnetic stirring is subjected to centrifugal separation, and a rough astaxanthin extraction solution is collected; salt is added to the rough astaxanthin extraction solution, standing is conducted atthe normal temperature to form the ionic liquid-salt aqueous two-phase system, liquid-liquid separation is conducted, and an ionic liquid enrichment phase is collected for obtaining an astaxanthin concentration solution; water is added to the astaxanthin concentration solution, standing is conducted, so that astaxanthin is precipitated, then filtering is conducted, and an astaxanthin precipitateis collected and dried. According to the extraction method, the content of astaxanthin extracted from shrimp shells is high, the extraction method is easy and convenient to implement, the astaxanthincan be extracted in a large scale, the condition is mild, and the method is environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of chemical extraction, in particular to a method for extracting astaxanthin by utilizing an ionic liquid-salt two-phase aqueous system. Background technique [0002] The full name of astaxanthin is 3,3'-dihydroxy-4,4'-diketone-β,β'-carotene, which belongs to non-vitamin A source carotenoids, insoluble in water, easily soluble in dichloromethane and acetone , Chloroform and most other organic solvents. Astaxanthin has high-efficiency anti-oxidation, anti-cancer, immune-enhancing and coloring functions. It has been widely used in health food, medicine, cosmetics and aquaculture, and has extremely high economic value and health care. [0003] The traditional astaxanthin extraction methods mainly include oil-soluble method, alkaline extraction method, organic solvent extraction method and supercritical carbon dioxide extraction method. The oil-soluble method uses astaxanthin’s good fat-soluble properties to e...

Claims

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Application Information

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IPC IPC(8): C07C403/24
Inventor 高静张莉莉彭锦学
Owner GUANGDONG OCEAN UNIVERSITY
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