Preparation method and application of autologous tumor vaccine
A tumor vaccine, autologous technology, applied in the fields of biotechnology and immunology, can solve the problems of lack of immunogenicity and specific autologous tumor vaccine preparation methods
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0106] Preparation of individualized tumor vaccines
[0107] Take 4T1 cells or Lewis lung cancer cells in logarithmic growth phase 1*10 7 After being irradiated with γ-rays (4-10Gy, 1.0Gy / min) for 30-60 minutes, heat in a water bath at 37-40°C for 20 minutes; at 37°C, 5% CO 2 After 48 hours in a constant temperature incubator, the cells were disrupted by ultrasonic waves, and Freund's adjuvant was added to prepare an emulsified agent, which is the tumor whole cell vaccine.
Embodiment 2
[0109] Individualized tumor vaccine for the treatment of subcutaneous breast cancer tumors in mice
[0110] In this example, the relevant indicators of cell and animal experiments confirmed that the tumor vaccine prepared by the method of the present invention has strong antigen presentation and the ability to stimulate antibody immune response. The specific results are as follows:
[0111] 1. Promote the production of anti-tumor antibodies
[0112] Experimental method: 3 weeks after conventional Balb / c mice were immunized with the tumor cell antigen prepared in Example 1, the plasma was taken and diluted, and added to an ELISA plate coated with tumor cells to resist mouse IgG-HRP and TMB substrate Display the antibody titer (1:100000 in this experiment).
[0113] The results show that compared with the tumor vaccine prepared by the control method, the tumor antigen prepared in Example 1 has a stronger ability to promote the production of anti-tumor antibodies ( figure 1 ):...
Embodiment 3
[0118] Inhibitory effect on tumors in mice
[0119] According to the principles of immunology, the mouse anti-tumor immunity test is divided into two steps:
[0120] 1. Tumor immune sensitization: inoculate the treated tumor antigen new tumor vaccine in the subcutaneous area of the mouse to be tested, and boost the immunization once after 14 days, and inoculate the homologous mouse tumor cells ( image 3 ).
[0121] 2. Immune anti-cancer effect: divided into two categories:
[0122] a. Inhibitory effect on carcinoma in situ: using BALB / c mice and 4T1 breast cancer cell lines;
[0123] b. Inhibitory effect on metastatic tumors: C57BL / 6 mice and Lewis lung cancer cell lines were used. Both Lewis and 4T1 cancer cells are highly malignant, fast-growing, difficult to control, and capable of forming lung metastases.
[0124] Judgment of anti-tumor effect:
[0125] 1. Judgment of curative effect on antigenic carcinoma:
[0126] a. In vivo tumor fluorescence quantification met...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com