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Application of butyric acid in preparing medicine for preventing and/or treating autoimmune diseases

An autoimmune, butyric acid technology, used in allergic diseases, anhydride/acid/halide active ingredients, bone diseases, etc., can solve the problems of patients' infection, inability to achieve effective disease relief, etc., to relieve symptoms, correct Effects of immune cell imbalance and reduced autoantibody production

Inactive Publication Date: 2019-05-21
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the treatment methods for autoimmune diseases and immune-related inflammatory diseases are still hormones, immunosuppressants and biological agents. Excessive immunosuppression cannot achieve effective disease relief and usually brings adverse reactions such as infection to patients

Method used

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  • Application of butyric acid in preparing medicine for preventing and/or treating autoimmune diseases
  • Application of butyric acid in preparing medicine for preventing and/or treating autoimmune diseases
  • Application of butyric acid in preparing medicine for preventing and/or treating autoimmune diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Intestinal butyrate levels significantly reduced in patients with rheumatoid arthritis

[0038] The content of short-chain fatty acids in feces of 24 normal subjects and 30 patients with rheumatoid arthritis was detected by gas chromatography-mass spectrometry (GC-MS). Methods as below:

[0039] 1. Standard product configuration and pretreatment

[0040] Accurately weigh the pure standards of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and hexanoic acid, and use ethyl acetate to prepare 0.5 μg / mL, 1 μg / mL, 5 μg / mL, 10 μg / mL, 20μg / mL, 50μg / mL, 100μg / mL, 200μg / mL eight mixed standard concentration gradients. Take 600 μL of the standard, add isocaproic acid with a final concentration of 500 μM as an internal standard, mix well and add to the injection bottle, and wait for gas chromatography-mass spectrometer (Gas Chromatography-Mass Spectrometer) GC-MS detection.

[0041] 2. Sample pretreatment

[0042] Thaw the sample on ice...

Embodiment 2

[0057] Butyric acid inhibits the development of experimental arthritis

[0058] method:

[0059] To study the preventive effect of butyric acid on experimental arthritis, the administration time is from the first day after the initial immunization to the 38th day after immunization, butyric acid is added to the mouse feed for feeding, and the amount of butyric acid added is 20wt% . The mice in the control group were fed normal diet. Morbidity record: After the booster immunization (ie, the 22nd day after the initial immunization), the incidence of arthritis in mice was observed every day, and the onset time and incidence rate were recorded. Incidence rate = (number of affected mice / total number of mice)*100. Arthritis scoring: observe the joint swelling of the mice, and score. The scoring was performed independently by two experimenters at the same time, and the average of the two scores was taken as the final score. Scoring joints include a total of four foot joints of th...

Embodiment 3

[0063] Butyric Acid Regulates Gut Immune Balance

[0064] (1) Butyric acid inhibits the expression of autoantibodies and pro-inflammatory factors and promotes the secretion of anti-inflammatory factors

[0065] The peripheral blood of the mice was collected, the serum was separated, and the contents of IgG type anti-II collagen antibody (anti-CII) and cytokines were detected by ELISA. The detection method is operated in accordance with the instructions of the kit, and the pre-test is carried out to determine the dilution factor of the sample, and then the formal experiment is carried out. The brand of CII kit is Chondrex, and the product number is Catalog#2036T; the brand of cytokine kit is Lianke.

[0066] The results showed that the expression of anti-CII antibody in the butyric acid intervention group was significantly reduced ( Figure 5 A, butyric acid group 17520±4400 vs disease control group CIA22385±1856), indicating that butyric acid can inhibit the production of au...

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Abstract

The invention provides an application of butyric acid in preparing a medicine for preventing and / or treating autoimmune diseases, and belongs to the technical field of autoimmune disease treatment. According to the embodiment of the invention, preventive intervention of butyric acid can obviously inhibit the occurrence and development of experimental arthritis, reduce the incidence rate of the arthritis by 74%, and protect articular synovial tissue from infiltration or erosion damage of inflammatory cells. The butyric acid can increase the expression of anti-inflammatory factor IL-10 while reducing the production of an autoantibody anti-CII and a pro-inflammatory factor IL-6. Furthermore, the butyric acid promotes the differentiation of joint lymph nodes and intestinal tract peyer's patches treg cells, and inhibits the expression of Tfh cells. The butyric acid can also down-regulate B cells of the germinal center of intestinal tract peyer's patches.

Description

technical field [0001] The invention belongs to the technical field of autoimmune diseases, and in particular relates to the application of butyric acid in the preparation of drugs for preventing and / or treating autoimmune diseases. Background technique [0002] Autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and Sjogren's syndrome (SS) are diffuse connective tissue or inflammatory diseases characterized by immune inflammation. The appearance of multiple pathogenic autoantibodies in serum and multisystem involvement are the main clinical features of this type of disease. For example, in the early stage of SLE patients, clinical symptoms such as low-grade fever, facial rash, fatigue, hair loss, and arthralgia are often manifested. In severe cases, neurological symptoms such as convulsions and syncope may occur, and chronic nephritis or even renal failure may develop in the late stage. The prevalence of this disease in my country is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/19A61P37/02A61P29/00A61P19/02
Inventor 栗占国何菁李静
Owner PEOPLES HOSPITAL PEKING UNIV
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