Preparation method and application of tupistra extract
A technology of open arrow and extract, which is applied in the field of preparation of open arrow extract, can solve the problems of poor antibacterial effect of the extract, high solvent toxicity, susceptibility to microorganisms, etc., and achieves broad antibacterial spectrum, high extraction rate and less residue Effect
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Embodiment 1
[0030] Open arrow rhizome coarse powder 18-30 mesh 100g, add 1000mL 95% ethanol (the volume fraction of ethanol is 95%), ultrasonic-assisted extraction at room temperature for 20min, soak at room temperature for 12h, stir well, then ultrasonicate at room temperature for 20min, then Placed in a water bath at 50°C for constant temperature extraction for 3h, suction filtration, and the same method was repeated twice; the filtrates were combined, concentrated under reduced pressure at 50°C to extract, freeze-dried to constant weight, and the yield was about 10%.
Embodiment 2
[0032] Open arrow rhizome coarse powder 18-30 mesh 200g, add 2000mL ethyl acetate, ultrasonic-assisted extraction at room temperature for 30min, then soak at room temperature for 18h, stir well, then ultrasonicate at room temperature for 20min, and then place it in a water bath at 55 ℃ constant temperature Extraction for 3 hours, suction filtration, and the same method was repeated twice; the filtrates were combined, concentrated under reduced pressure at 60°C to extract, freeze-dried to constant weight, and the yield was about 1.85%.
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