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Preparation and application of Lactobacillus gasseri with aquatic pathogenic bacterium antagonism

A technology of Lactobacillus gasseri and aquatic pathogenic bacteria, applied in the field of microorganisms, can solve problems such as no patent report of Lactobacillus gasseri, and achieve the effects of reducing bait coefficient, low preparation cost, and improving weight gain rate

Active Publication Date: 2019-05-14
江苏省苏微微生物研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no patent report on the application of Lactobacillus gasseri in aquaculture

Method used

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  • Preparation and application of Lactobacillus gasseri with aquatic pathogenic bacterium antagonism
  • Preparation and application of Lactobacillus gasseri with aquatic pathogenic bacterium antagonism
  • Preparation and application of Lactobacillus gasseri with aquatic pathogenic bacterium antagonism

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: the screening of strain

[0030] Allogenetic gibel carp samples were collected in Wuxi Ehu aquaculture pond, the fish body was dissected to take the intestinal tract, and physiological saline was added to make a homogenate, and a pipette gun was used to draw 0.1mL on the MRS solid medium, spread, and cover a culture medium at 37°C. According to the size of the colony, the well-growing colony was selected and screened repeatedly until a uniform single colony was obtained, which was named 88.

Embodiment 2

[0031] Embodiment 2: strain identification

[0032] (1) Morphological characteristics: Lactobacillus gasseri strain 88, Gram staining is positive, spherical or oval, (0.5-1.2) μm × (0.5-1.5) μm, no spores, no capsule, no movement . On the MRS plate, the bacteria are short rods with rounded ends, usually appearing alone and in pairs of short chains.

[0033] (2) Biochemical characteristics: Lactobacillus gasseri strain 88, Gram stain positive, can produce H 2 S, pyruvate, tryptophan, gelatin, glucose, sucrose, amygdalin were positive.

[0034] The strain can utilize galactose, glucose, mannose, lactose, aesculin, salicyl alcohol, cellobiose, maltose, N-acetyl-glucosamine, lactose, sucrose, trehalose, geranose, D-tag Sugar fermentation produces acid.

[0035] (3) 16S rRNA sequence analysis and phylogenetic tree construction: the 16S rRNA gene sequence of Lactobacillus gasseri strain 88, the length of the 16S rRNA gene is 1149bp, as shown in SEQ ID NO.1; and the nucleic acid ...

Embodiment 3

[0037] Embodiment 3: Bacteriostasis determination

[0038] The pathogenic bacteria and Lactobacillus gasseri 88 bacterial fluid were inoculated into the MRS medium, cultured at 30 ℃ for 48 hours, samples were counted at 0 h, 24 hours, and 48 hours, and the antagonism of Lactobacillus gasseri to the pathogenic bacteria was observed effect. The measurement results are shown in Table 1:

[0039] Table 1 Lactobacillus gasseri 88 and pathogenic bacteria co-culture assay results (CFU / mL)

[0040]

[0041] It can be seen from Table 1 that Lactobacillus gasseri 88 at a low concentration (9.5E+05) can inhibit the growth of different types of pathogenic bacteria at a high concentration. Inhibitory effect of Lactobacillus gasseri 88 on Listonia anguillarum, Vibrio parahaemolyticus, Vibrio alginolyticus, Aeromonas hydrophila, Aeromonas victoria, Aeromonas caviae after co-cultivation for 24 hours significantly. After co-cultivation for 48 hours, although the concentration of Lactoba...

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Abstract

The invention discloses preparation and application of Lactobacillus gasseri with aquatic pathogenic bacterium antagonism, and belongs to the technical field of microorganisms. A Lactobacillus gasseristrain 88 is obtained by screening and classified and named as Lactobacillus gasseri, and the former name is Lactobacillus gasseri. The strain can grow in culture pond water and bottom mud, and has the broad spectrum antagonism on various aquatic common pathogenic bacteria in freshwater culture. Due to culture activation, fermentation cultivation and preparation, powder and water are prepared, COD, ammonia nitrogen, nitrite, total phosphorus and total nitrogen in water can be effectively degraded due to cooperative use of the powder and the water, and growth of aquatic culture animals is promoted. The obtained Lactobacillus gasseri preparation is adopted as a water improver for aquaculture so that the content of contaminations in water can be effectively lowered, and the culture water quality is improved; when the strain is adopted as a fishery culture feed additive, the aquatic animal weight gain rate and the specific growth rate and aquatic animals can be remarkably increased, and the feed coefficient is remarkably lowered.

Description

technical field [0001] The invention relates to the preparation and application of a Lactobacillus gasseri preparation with antagonistic properties of aquatic pathogens, belonging to the technical field of microorganisms. Background technique [0002] In recent years, the rapid development of aquatic animal breeding in our country has greatly enriched people's material life. With the continuous improvement of the intensification of the breeding industry, various diseases occur frequently, and various chemical drugs and antibiotics are abused. The resulting problem of drug resistance of pathogenic bacteria, imbalance of flora in animals and antibiotic residues are becoming more and more serious. Antibiotics have become global pollutants, and their huge negative effects have brought serious economic losses to farmers, brought terrible hidden dangers to consumers' food safety, and hindered the sustainable development of the world's aquaculture industry. Microbial preparations ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/25
CPCY02A40/81
Inventor 张维娜施大林高亮于丹孙梅匡群张一平
Owner 江苏省苏微微生物研究有限公司
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