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Aspergillus niger and application in production of isomaltose hypgather

A technology of isomaltooligosaccharide and Aspergillus niger, which is applied in the field of fermentation engineering, can solve the problems of increased production cost and complicated production process, and achieve the effects of increased content, simple and easy cultivation method, and shortened conversion reaction time

Active Publication Date: 2015-09-02
GUANGXI NANNING ZHITIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to obtain a higher purity isomaltooligosaccharide product, it is necessary to use chromatographic separation and other methods for secondary separation on this basis to remove glucose and maltose from IMO-500 syrup to form IMO-900 type products, and its total oligomeric The content of isomaltose is about 90%, of which the effective trisaccharide content is still only about 45%, which still cannot meet the needs of the high-end market. In addition, the chromatographic separation operation also complicates the production process and greatly increases the production cost

Method used

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  • Aspergillus niger and application in production of isomaltose hypgather

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Aspergillus niger is cultivated and its application 1

[0032] (1) Strain culture:

[0033] Seed medium: cornstarch 50g / L, corn steep liquor dry powder 20g / L, pH 4.5.

[0034] Fermentation medium: corn starch 60g / L, corn steep liquor dry powder 30g / L, pH 4.5.

[0035] Aspergillus niger ( Aspergillus niger ) M1 is a fermentation strain, and the seeds are cultivated at 32°C, with a medium volume of 10%, a shaking table speed of 200rpm, and a seed age of 12h; the seeds are inoculated into the fermentation medium according to the inoculum size of 10% (v / v). , the amount of liquid in the medium was 20%, the rotation speed of the shaker was 200rpm, and after fermentation at 32°C for 36 hours, the fermentation broth was vacuum filtered to collect mycelium.

[0036] (2) Strain application

[0037] Free mycelium is used for transformation to produce isomaltooligosaccharides. Commercially available maltose syrup with a solid content of 30% by weight is placed i...

Embodiment 2

[0038] Embodiment 2: Aspergillus niger is cultivated and its application 2

[0039] (1) Strain culture:

[0040] Same as the bacterial classification cultivation of embodiment 1.

[0041] (2) Strain application

[0042] Free mycelium is used for transformation to produce isomaltooligosaccharides. Commercially available maltose syrup with a solid content of 30% by weight is placed in the reactor, and free mycelium is added according to the wet weight of the bacteria at 10% (w / v). , under the condition of 60 ℃, after conversion for 6 hours, the isomaltooligosaccharide syrup was obtained; the effective trisaccharide (IG+P+IG 3 ) content is 68.35%.

Embodiment 3

[0043] Embodiment 3: Aspergillus niger is cultivated and its application 3

[0044] (1) Strain culture:

[0045] Same as the bacterial classification cultivation of embodiment 1.

[0046] (2) Strain application

[0047] Immobilized cells were used to transform and produce isomaltooligosaccharide, and Aspergillus niger ( Aspergillus niger ) M1 free mycelium for immobilization. Put the cassava starch saccharification solution with a solid content of 20% in the reactor, add 20% (w / v) to the immobilized cells, and transform it at 50°C for 8 hours to obtain the isomaltooligosaccharide syrup ; Measured by high performance liquid chromatography, the effective trisaccharide (IG+P+IG 3 ) content is 45.72%.

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Abstract

The invention discloses an aspergillus niger which is classified and named aspergillus niger M1 with the preservation number being CCTCC NO: M2014421. The preservation date is September 23nd, 2014, and the preservation department is China Center for Type Culture Collection. Alpha-glucosidase produced by the aspergillus niger M1 strain is endoenzyme, and the aspergillus niger M1 strain can produce isomaltose hypgather containing more than 65% of active trisaccharide (IG+P+IG3) under the condition of high temperature. The required reaction time of preparing IMO-500 type isomaltose hypgather containing 35% of active trisaccharide is only half of the reaction time of preparing the isomaltose hypgather containing more than 65% of active trisaccharide. The alpha-glucosidase produced by the strain has advantages of excellent temperature characteristic, short glucoside conversion time, high content of the active trisaccharide in the product and the like.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a high-yield α-glucosidase Aspergillus niger ( Aspergillus niger ), and a method for using it to convert starch saccharification liquid or maltose syrup to prepare isomaltooligosaccharide. Background technique [0002] Isomaltooligosaccharides (IMO) is currently the most widely used and most demanded oligosaccharide functional sweetener. It has special physiological functions such as low calorie, anti-caries, and promoting the proliferation of intestinal bifidobacteria. It has been widely used in food, medicine and health products, feed and other industries. It is a mixture of a series of glucans with a degree of polymerization of 2-10, all of which have at least one α-(1,6) glycosidic bond in their molecules, and the most abundant component is isomaltose (Isomaltose) , IG), panose (Panose, P) and isomaltotriose (Isomaltotriose, IG 3 ), these three components ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/14C12R1/685
Inventor 梁智群陈桂光李玮曾伟
Owner GUANGXI NANNING ZHITIAN BIOTECH
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