Phaffiarhodozyma mutant strain MK19 of glucose metabolism de-repression high-yield astaxanthin and culturing method thereof

Abstract

The invention relates to a phaffiarhodozyma mutant strain MK19 of glucose metabolism de-repression high-yield astaxanthin and a culturing method thereof. The phaffiarhodozyma mutant strain MK19 is obtained from a phaffiarhodozyma wild-type test stain by adopting a 60Co-induced screening method, and the phaffiarhodozyma mutant strain MK19 is activated by solid seeds and liquid seeds, fermented and cultured to obtain mutant strain MK19 fermentation liquor. The cell density of the phaffiarhodozyma mutant strain MK19 reaches OD600 which is equal to 32, and the astaxanthin content is 918.53Mug / g, therefore, the astaxanthin content in the cell of the mutant strain is 22 times that of the wild-type strain. Besides, the strain has simple nutrient ingredient requirements and low medium cost, can simplify the production process of the astaxanthin on the industrial application and shorten the production cycle of the phaffiarhodozyma, thereby reducing the cost.

Description

【Technical field】 The invention belongs to the field of microbial biotechnology. More specifically, the present invention relates to a Phaffia mutant strain MK19 with derepressed glucose metabolism and high astaxanthin production and a culture method thereof. 【Background technique】 Astaxanthin is an orange-red carotenoid that widely exists in nature and has strong antioxidant activity, which is 500 times that of vitamin E. Animal experiments have shown that astaxanthin has various physiological effects such as inhibiting tumor occurrence and enhancing immune function; it can prevent and treat neurological diseases, such as Alzheimer's disease and Parkinson's disease, and can be used as a therapeutic agent for anti-aging and preventing arteriosclerosis and other diseases. Drugs or health care products; can be used to prevent and treat eye diseases, especially to significantly reduce the incidence of diabetic eye diseases. The US Food and Drug Administration (FDA) and the E...

AI Technical Summary

Problems solved by technology

However, this feeding method is complicated, and the production in this way in industrial production will prolong the fermentation cycle and increase labor and production costs.

Currently, most of the reported high-yielding astaxanthin mutants have an optimal glucose concentration of 1%-5% [see Hu, Z. et al., "World Journal of Microbiology and Biotechnology", 21:771-775 (2005); Kim, J.H. et al., Biosci Biotechnol Biochem, 69:1743-8 (2005); Ni, H., et al., J Zhejiang Univ Sci. B8:365-70 (2007); Reynders, M.B., D.E. Rawlings, and S.T.L.Harrison. "Biotechnology Letters", 19:549-552 (1997); Yamane, Y., et al., "Biotechnology Letters", 19:1109-1111 (1997)], and this Phaffia mutant strain also exists Many defects, such as the metabolic repression effect of high-concentration glucose, resulting in the addition of carbon sources in batches during the fermentation process, will increase the production cost of astaxanthin, etc.