Preparation method of antigen determinant/DOX double-template molecularly imprinted fluorescent nanoparticles with alpha-helix structure

An epitope and fluorescent nanotechnology, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, anti-tumor drugs, etc., can solve the problems of complex preparation process, achieve high specific surface area, and enhance recognition specific effect

Inactive Publication Date: 2019-05-07
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, there are many researches and developments on nanomaterials as drug carriers, but the preparation process is generally complicated.

Method used

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  • Preparation method of antigen determinant/DOX double-template molecularly imprinted fluorescent nanoparticles with alpha-helix structure
  • Preparation method of antigen determinant/DOX double-template molecularly imprinted fluorescent nanoparticles with alpha-helix structure
  • Preparation method of antigen determinant/DOX double-template molecularly imprinted fluorescent nanoparticles with alpha-helix structure

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for preparing α-helical epitope / DOX double-template molecularly imprinted fluorescent nanoparticles, specifically comprising the following steps:

[0028]1) Preparation of silicon nanoparticles, take 0.318g trisodium citrate and add 8mL glycerol, add 2mL 3-aminopropyltriethoxysilane (APTES) under the protection of argon, the above reaction is carried out in a microwave reactor, 15min Finally, silicon nanoparticles (Si NPs) are obtained;

[0029] 2) Purification of silicon nanoparticles: add 8 mL of silicon nanoparticles to 12 mL (0.5 mol / L) dilute hydrochloric acid, take the molecular cut-off of the dialysis bag to be 1000 for dialysis, change the water once every 8 hours, and change the water 3 times in total. The time is 24h overnight to obtain purified silicon nanoparticles (Si NPs);

[0030] 3) Add 6 mL of purified silicon nanoparticles to 40 mL of ethanol solution, add 0.667 mL of ammonia, add 0.8 mL of tetraethoxysilane (TEOS), and react at room temperat...

Embodiment 2

[0038] A preparation method of α-helical epitope / DOX double-template molecularly imprinted fluorescent nanoparticles, the synthesis steps are basically the same as in Example 1, the difference is: step 1) take 0.315g trisodium citrate and add 8mL glycerine alcohol.

[0039] The results of the final preparation of double-template conformational epitope-imprinted nanoparticles and the application of MIPs@DOX are similar to those in Example 1.

Embodiment 3

[0041] A preparation method of α-helical epitope / DOX double-template molecularly imprinted fluorescent nanoparticles, the synthesis steps are basically the same as in Example 1, the difference is: step 1) take 0.32g trisodium citrate and add 8mL glycerine alcohol.

[0042] The results of the final preparation of double-template conformational epitope-imprinted nanoparticles and the application of MIPs@DOX are similar to those in Example 1.

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Abstract

The invention discloses a preparation method of antigen determinant / doxorubicin hydrochloride (DOX) double-template molecularly imprinted fluorescent nanoparticles with an alpha-helix structure. Fluorescent nano silicon spheres wrapping silicon nano-particles are used as a carrier, P32 protein N-terminal conformation 9 peptides and doxorubicin hydrochloride are used as double-template molecules, and the double-molecular imprinted nano particles are prepared by adopting a precipitation polymerization method. According to the invention, the Si nano particles are wrapped in silicon dioxide to prepare fluorescent nano silicon spheres and the fluorescent double-template molecularly imprinted nano particles, and the fluorescence characteristic can be used for fluorescence imaging of tumor cells.A polypeptide with an alpha-helix structure constructed by using trifluoroethanol is used as a template molecule, so that target identification of target tumor cells can be effectively improved. Thenano particles are prepared by adopting the conformation polypeptide and the doxorubicin hydrochloride as double templates, targeted medicine delivery is realized while targeted specific recognition of tumor cells is realized, the amount of medicines reaching target sites is increased, side effects are reduced, the medicine utilization rate is improved, and the treatment effect is enhanced.

Description

technical field [0001] The invention belongs to the field of preparation of nanometer materials, in particular to a preparation method of α-helical epitope / DOX double-template molecularly imprinted fluorescent nanoparticles. Background technique [0002] Molecular imprinting technology refers to a technology that embeds template molecules into a polymer matrix to generate specific recognition cavities. When the template is dissociated from the polymer matrix, not only the shape of the template is preserved, but also the imprinted polymer can specifically recognize the template molecule. This technique has been successfully used to construct various polymeric materials that specifically bind to peptide and protein templates, providing a promising carrier for molecular design, drug discovery and drug delivery. See: Piotr.Luliński.Mater.Sci.Eng.C Mater.Biol.Appl.2017,76:1344-1353; H.C.Chen,J.Kong,D.Y.Yuan,G.Q.Fu.Biosens.Bioelectron.2014,53:5- 11; Maya.Z, Andrew.J.B., Peter.C....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F220/54C08F222/38C08F220/04C08J9/26A61K9/51A61K47/32A61K31/704A61P35/00
Inventor 李文友秦亚婷张玉奎何锡文
Owner NANKAI UNIV
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