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Preparation method of composite protein powder with high amino acid grade

A compound protein powder, high amino acid technology, applied in protein food processing, animal protein processing, vegetable protein processing and other directions, to achieve the effect of full and efficient utilization

Inactive Publication Date: 2019-04-26
刘良忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the above-mentioned protein powders currently on the market are not suitable for the elderly and patients with poor protein digestion function, and chronic kidney disease (CKD) people who have limited needs for sodium, potassium, and phosphorus in food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Prepare rice protein and egg white protein at a mass ratio of 6:4 to make a water suspension with a protein concentration of 8%, and heat-treat at 100°C for 10 minutes to denature the protein. 3 times, colloid mill micronization treatment 3 times, so that the protein particle size is below 10μm; adjust the pH of the mixed protein solution to 2-3, the temperature is 35 ℃, the ratio of papain and pepsin is 1:1, and the enzyme addition amount is the substrate protein 2%, ultrasonic frequency 30kHz, power density 100W / L, stirring speed 30rpm, ultrasonic time 60min, to obtain a protein hydrolyzate with a degree of hydrolysis of 9%;

[0032] The protein hydrolyzate is instantly sterilized at 121°C for 9 seconds at ultra-high temperature, cooled to 37°C, and protease hydrolyzate is added with 10 8 The number of cfu live bacteria, using three joint fermentations of Bacillus subtilis-yeast-lactic acid bacteria, the ratio of the three strains is 1:1:1, the yeast is Saccharomyces ...

Embodiment 2

[0036] Mix wheat protein, soybean protein, and duck egg white protein evenly in a mass ratio of 7:2:1, prepare a water suspension with a protein concentration of 6%, heat at 95°C for 15 minutes to denature the protein, and micronize it with a colloid mill 4 Repeat to bring the protein particle size down to 15 μm.

[0037]Adjust the pH of the mixed protein solution to 45°C, the mass ratio of alkaline protease and trypsin is 1:1, the amount of enzyme added is 3% of the substrate protein, the ultrasonic frequency is 50kHz, the power density is 250W / L, and the ultrasonic time is 60min. A protein hydrolyzate with a degree of hydrolysis of 25% is obtained;

[0038] The protein hydrolyzate is sterilized at 95°C for 10 minutes, cooled to 38°C, and protease hydrolyzate is added with 10 7 The ratio of cfu live bacteria is yeast-lactic acid bacteria joint fermentation, the ratio of yeast-lactic acid bacteria is 1:1, the yeast is grape juice yeast, and the lactic acid bacteria are Strept...

Embodiment 3

[0043] Corn protein, pea protein and oyster protein are mixed evenly at a mass ratio of 5:4:1, prepared into a water suspension with a protein concentration of 5%, heated at 92°C for 16 minutes to denature the protein, and finely processed by a high-pressure homogenizer. Mass pressure 40Mpa, temperature 50°C, 3 times, so that the protein particle size is about 10μm.

[0044] Adjust the pH of the mixed protein solution to 7.5, the temperature is 37°C, the mass ratio of neutral protease, papain and ficin is 2:1:1, the amount of enzyme added is 4% of the substrate protein, the ultrasonic frequency is 40kHz, and the power density is 150W / L , the ultrasonic time is 70min, and the protein hydrolyzate with a degree of hydrolysis of 20% is obtained;

[0045] The protein hydrolyzate is sterilized at 123°C for 15 seconds, cooled to 41°C, and the protease hydrolyzate is added with 5×10 6 The ratio of the number of viable cfu bacteria is combined fermentation of Bacillus subtilis and lac...

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Abstract

The invention provides a preparation method of composite protein powder with a high amino acid grade. The preparation method specifically comprises the following steps: performing micronization treatment on raw material protein by a colloid mill and / or a high-pressure homogenizer, heating the raw material protein to denature the protein, performing countercurrent ultrasonic assisted enzymatic hydrolysis through single protease or multi-enzyme combination, performing individual or joint fermentation of yeast, lactic acid bacteria and bacillus subtilis to remove stinking and peculiar smell, performing ultrafiltration and ion exchange resin desalting, evaluating and supplementing essential amino acids, performing enzymatic polymerization and reconstitution reaction, and performing drying or performing drying and mixing to obtain a high-quality protein product with a reasonable proportion of the essential amino acids and an amino acid grade being 95 or above. The obtained composite proteinpowder has the high essential amino acid grade, belongs to high-quality protein, is easy to digest and absorb, has low sodium content, is suitable for people who need the high-quality protein, elderly people with a low protein digestion function, and persons under rehabilitation; the protein powder with low contents of sodium, potassium and phosphorus is suitable for people suffering from chronickidney disease (CKD) and the like; the product also has the effects of resisting oxidation, lowering blood cholesterol, strengthening the body and enhancing immunity.

Description

technical field [0001] The invention relates to the technical field of food raw material processing, in particular to a method for preparing composite protein powder with high amino acid score. Background technique [0002] Protein is an indispensable substance in the human body, the material basis of life, the basic organic matter that constitutes cells, and the main bearer of life activities. Protein is one of the seven essential nutrients for the human body. Protein exists in various parts and cells of the body, and the content in the human body is about 17%-21%. Different proteins have different functions and properties. Protein functions include: forming and repairing human tissues, providing essential amino acids, regulating body fluids and maintaining acid-base balance, synthesizing physiologically active substances, enhancing immunity, and providing energy. There are many sources of protein, including grains, beans, eggs, meat, milk, aquatic products, etc. The Ch...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J3/04A23J3/08A23J3/14A23J3/16A23J3/18A23J3/20
CPCA23J3/04A23J3/08A23J3/14A23J3/16A23J3/18A23J3/20A23J3/341A23J3/343A23J3/346A23J3/347A23V2400/133A23V2400/121A23V2400/151A23V2400/137A23V2400/147A23V2400/143A23V2400/313A23V2400/231A23V2400/321A23V2400/413A23V2400/427A23V2400/125A23V2400/123A23V2400/513A23V2400/529A23V2400/517A23V2400/519A23V2400/175A23V2400/113A23V2400/533A23V2400/249A23V2400/169
Inventor 刘良忠王梦颖陶鑫
Owner 刘良忠
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