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Ultra-high performance liquid chromatography detecting method for traditional Chinese medicine composition

A detection method and composition technology, applied in the field of traditional Chinese medicine, can solve problems such as the inability to comprehensively and effectively reflect the quality consistency and effectiveness of traditional Chinese medicine compound preparations

Active Publication Date: 2019-04-23
SHIJIAZHUANG YILING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Chinese medicine compound preparations often include multi-flavored medicinal materials, and some preparations even include animal drugs or mineral drugs, and the complexity of the chemical components contained in them is far more than that of single medicinal materials.
In fact, more and more pharmaceutical researchers realize that the use of microscopic identification, thin-layer chromatography identification and quantitative analysis of a single index component is far from being able to fully and effectively reflect the quality consistency and effectiveness of traditional Chinese medicine compound preparations.

Method used

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  • Ultra-high performance liquid chromatography detecting method for traditional Chinese medicine composition
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  • Ultra-high performance liquid chromatography detecting method for traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prescription: 255g forsythia, 255g honeysuckle, 85g roasted ephedra, 85g fried bitter almond, 255g gypsum, 255g isatis root, 255g Mianma Guanzhong, 255g houttuynia cordata, 85g patchouli, 51g rhubarb, 85g rhodiola, mint Brain 7.5g, licorice 85g;

[0035] Preparation method: the above thirteen flavors, patchouli plus water distillation extract volatile oil, collect the volatile oil, filter the water extract, and set aside; forsythia, ephedra, houttuynia, and rhubarb are extracted twice with 70% ethanol, and the first time 2 hours, 1.5 hours for the second time, filter the extract, combine, recover ethanol, and set aside; add honeysuckle, gypsum, isatis root, Mianmaguanzhong, licorice, rhodiola, add water and boil until boiling, add fried bitter almonds, and boil Second time, 1.5 hours for the first time, 1 hour for the second time, filter the decoction, combine the filtrates, add the aqueous solution after patchouli oil extraction, concentrate to a relative concentration...

Embodiment 2

[0069] Example 2 Correlation analysis between chemical evaluation and biological evaluation of the quality of traditional Chinese medicine composition

[0070] The present invention uses PCR technology to measure IL-6 and COX-2 in mouse lung tissue, and finds that the composition capsule of the present invention can significantly reduce the expression of IL-6 and COX-2 in the lung tissue of viral pneumonia model mice , and present a dose-dependent relationship, so IL-6 and COX-2 are used as indicators for biological evaluation of the quality of the Chinese medicine composition capsules of the present invention.

[0071] Taking the contents of seven chemical components (chlorogenic acid, rutin, forsythiaside A, forsythiaside, isochlorogenic acid C, isochlorogenic acid B, and caffeic acid) in the traditional Chinese medicine composition as independent variables, biological The potency is the dependent variable, and 40 batches of traditional Chinese medicine compositions are used...

Embodiment 3

[0079] Recipe and preparation method are with embodiment 1.

[0080] Detection method: the chromatographic column is Waters Acquity HSS T3 column (2.1×100mm, 1.7μm), the mobile phase is a mixture of methanol (A) and 0.1% (v / v) phosphoric acid aqueous solution (B), and the gradient elution program is: 0.00 –8.00 minutes: 12~20%A; 8.01–15.00 minutes: 20~22%A; 15.01–20.00 minutes: 22~28%A; 20.01–25.00 minutes: 28~35%A; 25.01–30.00 minutes: 35~ 43%A; 30.01–35.00 minutes: 43~50%A; 35.01–40.00 minutes: 50~60%A; detection wavelength 210nm, injection volume 2.0μL, flow rate 0.20mL / min, column temperature 30℃, column equilibrium The time is 7 minutes.

[0081] Preparation of reference solution: Accurately weigh an appropriate amount of chlorogenic acid, caffeic acid, rutin, isochlorogenic acid B, isochlorogenic acid C, forsythiaside A and forsythin In a 25mL brown volumetric flask, add chromatographic grade methanol to make solutions containing 60μg, 75μg, 68μg, 125μg, 64μg, 80μg, an...

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Abstract

The invention provides a method for detecting a traditional Chinese medicine composition. According to the method, seven components of chlorogenic acid, caffeic acid, rutin, isochlorogenic acid B, isochlorogenic acid C, forsythiaside A and phillyrin in the traditional Chinese medicine composition are detected through an ultra-high performance liquid chromatography, and the quality of the traditional Chinese medicine composition can be represented more comprehensively.

Description

technical field [0001] The invention relates to a detection method of a traditional Chinese medicine composition, belonging to the field of traditional Chinese medicine. Background technique [0002] The quality control means of traditional Chinese medicine compound preparations are mainly the qualitative and quantitative analysis of the microscopic identification, inspection, and content determination of individual raw medicinal materials in the preparation. However, traditional Chinese medicine compound preparations often include multi-flavored medicinal materials, and some preparations even include animal or mineral drugs, and the complexity of the chemical components contained in them is far more than that of single medicinal materials. In fact, more and more pharmaceutical researchers realize that the use of microscopic identification, thin-layer chromatography identification and quantitative analysis of a single index component is far from being able to fully and effec...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N2030/146
Inventor 王伽伯肖小河张海珠牛明马永刚王宏涛田书彦
Owner SHIJIAZHUANG YILING PHARMA
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