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Bio-based metal organic framework assembly esterase catalyst, preparation method and application of catalyst in biodiesel synthesis

An organic framework, biodiesel technology, applied in the biological field, can solve problems such as affecting enzyme catalytic activity and enzyme shedding, and achieve the effect of promoting energy substitution, strong stability and good biocompatibility

Inactive Publication Date: 2019-04-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these two methods have their own defects, such as physical adsorption, the enzyme is easy to fall off from the MOF framework, and the covalent linkage will affect the catalytic activity of the enzyme.

Method used

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  • Bio-based metal organic framework assembly esterase catalyst, preparation method and application of catalyst in biodiesel synthesis
  • Bio-based metal organic framework assembly esterase catalyst, preparation method and application of catalyst in biodiesel synthesis
  • Bio-based metal organic framework assembly esterase catalyst, preparation method and application of catalyst in biodiesel synthesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve 500mg of adenine in 5mL of boiling water at 100°C, then quickly pour the adenine solution into 20mL of 25°C aqueous solution dissolved in 10mg of thermophilic esterase QLM and 200mg of zinc acetate, and react at room temperature for 48h. After the reaction was completed, the system was centrifuged at 8000r / min for 5min to collect the precipitate, which was washed three times with distilled water, and the freeze-dried powder was the final product QLM@Bio-MOF with a mass of 250mg.

[0026] By a similar method, a bio-based MOF material (me-Bio-MOF) without thermophilic esterase was prepared, except that the thermophilic esterase QLM was not added.

[0027] From figure 1 The scanning electron microscope of the synthesized product found that the QLM@Bio-MOF catalyst synthesized by the above method has regular morphology, and its particle size is between 800nm ​​and 1.5μm.

Embodiment 2

[0029] Dissolve 1000mg of adenine in 10mL of boiling water at 100°C, then quickly pour the adenine solution into 200mL of 100mg of QLM and 400mg of zinc acetate dissolved in an aqueous solution at 25°C. Reaction at room temperature for 48h. After the reaction was completed, the system was centrifuged at 8000r / min for 5min to collect the precipitate, which was washed three times with distilled water, and the freeze-dried powder was the final product QLM@Bio-MOF with a mass of 450mg.

[0030] By a similar method, a bio-based MOF material (me-Bio-MOF) without thermophilic esterase was prepared, except that the thermophilic esterase QLM was not added.

[0031] From figure 2 The results of scanning electron microscopy of the synthesized products found that the QLM@Bio-MOF synthesized by equal proportion of raw material input was regular and uniform in appearance, with particle size between 800nm ​​and 1.5μm. Compared with the QLM@Bio-MOF obtained by the small-batch synthesis met...

Embodiment 3

[0033] After fully drying the QLM@Bio-MOF and me-Bio-MOF synthesized in Example 1, the thermogravimetric experiment was carried out. The TAQ500 detection instrument was used to raise the temperature at a rate of 10°C / min in a nitrogen environment. The obtained thermogravimetric curve is as follows: figure 2 shown.

[0034] From image 3 It can be found that QLM@Bio-MOF has obvious weight loss at 200-500 °C, which is about 20% higher than that of me-Bio-MOF, and the higher weight loss represents the weight ratio of the enzyme. Therefore, according to the thermogravimetric curve, it is calculated that the loading amount of QLM in QLM@Bio-MOF is about 20%.

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Abstract

The invention relates to a bio-based metal organic framework assembly esterase catalyst, a preparation method and application of the catalyst in biodiesel synthesis, belonging to the biotechnologicalfield. Thermophilic esterase QLM is immobilized into a bio-based MOF material, and the esterase immobilized into the MOF is discovered to still have good catalytic activity through activity detection,and can successfully catalyze the hydrolysis of ester p-NPC (4-nitrophenyl caprylate); the esterase-MOF assembly serves as a catalyst to catalyze the transesterification of sunflower oil and methanol, and successfully synthesizes biodiesel with good performance. After loaded with an enzyme and entering the bio-based MOF, the thermophilic esterase QLM has better thermal stability and pH tolerance;the immobilized enzyme serves as a catalyst, can successfully achieve the synthesis of biodiesel, has higher catalytic efficiency than a free enzyme at a high oil / methanol proportion, and simultaneously has good operational stability and recyclability.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bio-based metal-organic framework-assembled esterase catalyst based on biomimetic mineralization technology, a preparation method and its application in biodiesel synthesis. Background technique [0002] Biodiesel refers to vegetable oil (such as sunflower oil, rapeseed oil, soybean oil, peanut oil, corn oil, cottonseed oil, etc.), animal oil (such as fish oil, lard, tallow, sheep oil, etc.), waste oil or microbial oil and Fatty acid methyl or ethyl esters formed by transesterification of alcohols. Biodiesel is a typical "green energy", which has the characteristics of good environmental performance, good engine starting performance, good fuel performance, wide source of raw materials, and renewable. Vigorously developing biodiesel has important strategic significance for sustainable economic development, promotion of energy substitution, and reduction of environmental...

Claims

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Application Information

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IPC IPC(8): B01J31/22B01J35/10C11C3/10C11C3/04C10L1/02
CPCC10L1/02C11C3/04C11C3/10B01J31/1691B01J31/2213B01J2531/54B01J35/61Y02E50/10
Inventor 李全顺李青柏韶伟杨艳姜淋杨洁冰
Owner JILIN UNIV
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