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Preparation and application of ferroferric oxide composite metal chelate mimic enzyme

A technology of ferroferric oxide and composite metal, which is applied in the field of nanotechnology and analysis and detection, can solve the problems of complex analysis process, long analysis time and high analysis cost, and achieve the effect of simple separation process, good selectivity and high sensitivity

Active Publication Date: 2019-03-22
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, detecting H 2 o 2 Especially important, there are a variety of detection methods nowadays, such as spectrophotometry, electroanalysis, resonance light scattering analysis, chromatography, etc., but these methods have some disadvantages, such as high analysis cost, long analysis time, and analysis process. Complexity, low signal-to-noise ratio, low sensitivity, poor selectivity, etc., it is difficult to meet the domestic and foreign requirements for H 2 o 2 Further requirements for testing

Method used

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  • Preparation and application of ferroferric oxide composite metal chelate mimic enzyme
  • Preparation and application of ferroferric oxide composite metal chelate mimic enzyme
  • Preparation and application of ferroferric oxide composite metal chelate mimic enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1, Fe 3 o 4 -Cu 2+ Preparation of mimetic enzyme

[0038] Step 1, weigh 2.162gFeCl 3 ·6H 2 O, 5.4 g of sodium acetate trihydrate and 1.0 g of sodium lignosulfonate were dissolved in 70 mL of ethylene glycol, and the mixture was continuously stirred until completely dissolved;

[0039] Step 2. Transfer the solution obtained in step 1 to a high-pressure reactor equipped with a polytetrafluoroethylene liner, put it into an oven that has been heated to 200 ° C, react for 12 hours, and then cool to room temperature with the furnace;

[0040] Step 3. Pour out the supernatant in the reaction kettle, take out the precipitate, wash with ethanol and deionized water at least three times, and dry at 60°C for 8 hours to obtain the sample Fe 3 o 4 -SL, using SEM to characterize it, it can be seen that it is a microsphere with a particle size of about 600nm, as shown in Figure 1 (A);

[0041] Step 4, under neutral environment, get the Fe that 0.02g step 3 obtains 3 o ...

Embodiment 2

[0044] Example 2, Fe 3 o 4 -Cu 2+ The detection steps of the simulated enzyme as a peroxidase to hydrogen peroxide are as follows:

[0045] Step (1), the Fe obtained in Example 1 3 o 4 -Cu 2+ The simulated enzyme is dispersed in water to produce 0.8mg / mL Fe 3 o 4 -Cu 2+ Simulated enzyme dispersion;

[0046] Step (2), get 50 μ L of Fe prepared in step (1) 3 o 4 -Cu 2+ The simulated enzyme stock solution was added to 1850 μL of 0.1M HAc-NaAc buffer solution (pH=3); then 50 μL of 32 mM 3,3',5,5'-tetramethylbenzidine salt was added to the reaction system Hydrogen peroxide (TMB) was used as the colorimetric reaction substrate; 50 μL of hydrogen peroxide solution with concentrations of 0.2mM, 0.3mM, 0.4mM, 0.6mM, 1mM, 2mM, and 3mM was added, and incubated at 45°C for 35 minutes to obtain a concentration of 5μM, 7.5μM, 10μM, 15μM, 25μM, 50μM, 75μM hydrogen peroxide solution;

[0047] Step (3), get the absorbance value of known hydrogen peroxide concentration solution tha...

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Abstract

The invention relates to a preparation and an application of a ferroferric oxide composite metal chelate mimic enzyme. According to the invention, a hydrothermal method is adopted for compounding Fe3O4-SL, wherein a big RCOO- group is derived from SL molecule and is attached to the surface of Fe3O4 powder; metal ions are absorbed by the Fe3O4-SL under a mild condition so as to form a nanometer composite material; due to static electricity and spatial effect, particles have excellent dispersity; the prepared Fe3O4 composite metal chelate has excellent microstructure and small grain size and hasexcellent peroxidase catalysis capacity; the separation process is simple, convenient and stable and the Fe3O4 composite metal chelate is reusable; a method for conveniently detecting hydrogen peroxide with high sensitivity and low cost is established on the basis of excellent peroxidase activity of Fe3O4 composite metal chelate mimic enzyme; the method has the advantages of mild reaction conditions, high detection speed, high sensitivity, high selectivity, capability of realizing visual rapid recognition and detection for hydrogen peroxide, and higher use value.

Description

technical field [0001] The invention belongs to the technical field of nanotechnology and analysis and detection, and in particular relates to the preparation and application of a ferric oxide complex metal chelate mimetic enzyme. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is one of the most important reactive oxygen species, and has been widely used in many fields such as biology, chemistry, pharmacy, food engineering, and environmental testing, and it is also because of its excessive use in many fields, which directly or indirectly leads to many health problems. and environmental issues. Therefore, detecting H 2 o 2 Especially important, there are a variety of detection methods nowadays, such as spectrophotometry, electroanalysis, resonance light scattering analysis, chromatography, etc., but these methods have some disadvantages, such as high analysis cost, long analysis time, and analysis process. Complexity, low signal-to-noise ratio, low sensitivit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/28B01J37/10G01N21/78G01N21/31
CPCB01J31/28B01J37/10G01N21/31G01N21/78
Inventor 刘红英祝浪浪马焕樊凯李杜鹃
Owner HANGZHOU DIANZI UNIV
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