Preparation method of mesenchymal stem cell fragmentation factor freeze-dried powder with multiple tissue sources

Abstract

The invention provides a preparation method of mesenchymal stem cell fragmentation factor freeze-dried powder with multiple tissue sources. According to the method, mesenchymal stem cells are separated from adipose tissues, mveloid tissues and umbilical cord tissues of a mammal, purified, and subjected to mixed culture, in the mixed culture process, supernate is collected and cultured in every stages, stem cells obtained through mixed culture are subjected to ultrasonication, stem cell fragmentation factors are collected, through two-time freeze-drying concentration treatment, the stem cell fragmentation factor freeze-dried powder is prepared, the whole freeze-drying process is maintained at 20 DEG C or below, heating is conducted step by step slowly, effects of temperature change on the activity of the stem cell factors are prevented, in a low-temperature low-pressure environment, crystal water slowly sublimates, in the presence of a freeze-drying protective additive, other componentsof the stem cell factors are not reduced, the activity is not affected, a compound which is easy to quantify and rich in mesenchymal stem bioactive factors is obtained, and the problem that when stemcell factors are concentrated, cell factor type loss is caused by different molecular weights of the stem cell factors is solved.

Description

technical field [0001] The invention belongs to the technical field of animal stem cells, and in particular relates to a preparation method of freeze-dried powder of mesenchymal stem cell lysing factor derived from multiple tissues. Background technique [0002] Mesenchymal stem cells are adult stem cells with high self-renewal ability and multi-directional differentiation potential, which exist in biological tissues such as umbilical cord, bone marrow, and dental pulp. Mesenchymal stem cells produce effects on surrounding adjacent cells by secreting various cytokines. Mesenchymal stem cells can secrete a variety of cytokines, such as SCF promoting stem cell growth factor; HGF hepatocyte growth factor; BFGF basic fibroblast growth factor; NGF nerve growth factor; BDNF brain-derived neurotrophic factor; IGF insulin-like growth factor VEGF vascular endothelial growth factor; Ang angiogenin; MCP chemokine; LIF9 leukemia inhibitory factor; EPO erythropoietin; SDF-1 stromal cell...

AI Technical Summary

Problems solved by technology

However, when the stem cell factor is concentrated, the type of cytokine is lost due to the different molecular weight of the stem cell factor; the cost of cold chain transportation in the liquid state is high, the preservation and maintenance of activity is difficult; the quantification is not accurate when used, etc.

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