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A preparation of rare ginseng saponin rk 3 Methods

A technology of rare ginseng saponins and ginseng, which is applied in the field of bioengineering, can solve the problems of difficult control of reaction conditions, complex reactions, and high cost of substrates, and achieve the effects of reducing extraction and purification steps, mild and controllable reaction conditions, and large yields

Active Publication Date: 2021-02-09
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Current pharmacological research shows that the rare ginseng saponin Rk 3 It has obvious inhibitory effect on liver cancer cells, can also be used to prevent and treat angiogenesis diseases, and has good curative effect on telangiectasia, but the rare ginseng saponin Rk in raw ginseng 3 The content is very low, and because of its complex structure, it is impossible to realize the total chemical synthesis by using existing science and technology. Currently, the rare saponins Rk of ginseng are prepared 3 Most of the methods are chemical high temperature and high pressure cracking method, common separation and purification method and panaxatriol type saponin biotransformation method
However, the chemical high-temperature and high-pressure cracking method has complex reactions, many side reactions, and difficult to control the reaction conditions; the ordinary separation and purification method has low extraction efficiency and cannot obtain a large amount of rare ginseng saponins Rk 3 ; Panaxatriol-type saponin biotransformation method, high substrate cost, low utilization rate of total ginseng saponins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Example 1: The influence of different medicinal fungi on the process

[0031]Thirteen kinds of medicinal fungi (Boletus edulis, Fungus, Shiitake 1500, Pleurotus eryngii, Pleurotus eryngii, Pleurotus eryngii, tree chicken, tree tongue, Yunzhi, Cordyceps, Grifola frondosa, white tree flower) and Ginseng is fermented to prepare the primary product according to the process of the present invention (the process conditions of each strain are the same), and the growth state of each medicinal fungus and the Rk in the primary product are determined.3The content is determined, and the results are shown in Table 1 and Table 2;

[0032]Table 1

[0033]

[0034]

[0035]Table 2

[0036] Bacteria RK 3 content%

Original ginseng herbs 0.0429 Pleurotus ostreatus 0.1309 Pleurotus eryngii 0 Bailing Mushroom 0 Cordyceps sinensis 0.7221 Yunzhi 0.2075

[0037]It can be seen from Table 1 and Table 2 that the bolete, green mushroom, tree chicken, shiitake mushroom 1500, Grifola frondosa and white tree flower did not ...

Embodiment 2

[0039]Example 2: Optimization of the best process conditions of the present invention

[0040]The initial water content of the original ginseng medicinal materials, the volume-to-mass ratio of the seed liquid and the original ginseng medicinal materials, and the cultivation temperature are used as influencing factors to optimize. Other conditions are the same. The results are shown in Table 3-5;

[0041]Table 3 Factor level table

[0042]

[0043]Table 4 Orthogonal experiment and results

[0044]

[0045]Table 5 Comprehensive score analysis of variance

[0046]

[0047]

[0048]F1-0.10(2,2) = 9.00F1-0.05(2,2)=19.00F1-0.01(2,2)=99.00

[0049]From the results in Table 3-5, it can be seen that under the process conditions of the present invention, compared with the original ginseng medicinal materials, it can significantly increase RK3Content; from the point of view of the influencing factors of the process conditions, the primary and secondary order of the factors that affect the preparation process is: seed liqui...

Embodiment 3

[0050]Example 3: Preparation of ginsenoside Rk under the best process conditions of the present invention3

[0051]The configuration of seed liquid: the medium formula is glucose 2%, KH2PO40.1%, MgSO40.1%, peptone 0.5%, yeast powder 0.2%, vitamin B10.1%, natural pH. Place the above substances in a large beaker, add appropriate amount of water to make seed liquid, after the seed liquid cools, divide it into 250ml jars, place it in a pressure steam sterilizer after wrapping, and sterilize at 121°C for 30 minutes . After cooling, insert 3-4 pieces of Cordyceps strains on the preserved slope in the aseptic operation table. Put the liquid culture medium with the inclined surface strains into a constant temperature shaking incubator, and cultivate for 4-5 days in the dark at 150r / min, 26℃, and 60% humidity. When the seed liquid becomes cloudy, viscous, and light yellow, Take it out for use.

[0052]Pre-treatment of the original ginseng medicinal materials: take the original ginseng medicinal ma...

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Abstract

The invention relates to the technical field of bioengineering, and discloses a method for preparing rare ginseng saponins Rk 3 Methods. The method of the present invention firstly prepares the Cordyceps sinensis seed liquid, then inoculates the sterilized original ginseng medicinal material and cultivates it until it is covered with hyphae, takes out the original ginseng medicinal material covered with mycelium, and dries it to obtain the rare ginseng saponin Rk 3 The primary product, the primary product is separated and purified to obtain the rare ginseng saponin Rk 3 . The invention combines the medicinal fungus Cordyceps sinensis and the original ginseng to produce ginsenoside Rk 3 , thus confirming the ability to prepare high-content ginsenoside Rk 3 Compared with the ginsenoside Rk in the original ginseng 3 content increased by 22 times. The method of the invention can effectively and directly utilize the original ginseng medicinal materials, reduce the extraction and purification steps in the early stage, and the reaction conditions are mild and controllable, and the Rk 3 The output is large and the purity is high.

Description

Technical field[0001]The invention relates to the technical field of bioengineering, in particular to a preparation of ginseng rare saponin Rk3Methods.Background technique[0002]Ginseng (Panax ginseng C.A. Meyer) is the root of ginseng, a perennial herbaceous plant in the Araliaceae family, and has been known as the "King of Herbs" since ancient times. The chemical components of ginseng are mainly ginsenosides, ginseng polysaccharides, etc., with complex components, wide biological activities, unique pharmacological effects, anti-cancer and anti-tumor, regulating the central nervous system and cardiovascular and cerebrovascular systems, improving the digestive system, promoting metabolism, and improving immunity. Anti-aging and nourishing effects.[0003]The current pharmacological research shows that the rare ginsenoside Rk3It has a significant inhibitory effect on liver cancer cells, and can also be used to prevent and treat angiogenesis diseases. It also has a good effect on telangi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/645
CPCC12P33/20
Inventor 邱智东徐伟董雪莲
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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