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Saffron petal total polysaccharide and extraction method and application thereof

An extraction method, the technology of saffron, is applied in the direction of pharmaceutical formulations, cosmetic preparations, medical preparations containing active ingredients, etc., which can solve the problems of low activity of ingredients, low extraction rate, low yield, etc., and achieve high in vitro antioxidant capacity Effect

Pending Publication Date: 2019-02-12
JINHUA SHOUXIANGU PHAMACEUTICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its style has been dubbed "Plant Gold" by the world because of its extremely low yield and magical medicinal effect. At the same time, a large number of non-medicinal parts-petals have become by-products in the pharmaceutical industry of saffron, resulting in a great waste of resources.
[0003] The prior art extracts the active components of saffron petals, the extraction rate is low, and the activity of the components obtained by extraction is low

Method used

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  • Saffron petal total polysaccharide and extraction method and application thereof
  • Saffron petal total polysaccharide and extraction method and application thereof
  • Saffron petal total polysaccharide and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Accurately weigh 3 portions of air-dried saffron petal powder after alcohol extraction, 2g each, and put them in 3 similar triangular flasks respectively, add 40mL of distilled water according to the ratio of material to water 1:20, stir and mix well with a glass rod . After sealing with plastic cling film, place it in a water bath at 100°C for extraction for 3 hours. Combine the filtrates filtered through the Buchner funnel and repeat the suction filtration again, then concentrate under reduced pressure in a rotary evaporator to the minimum volume, add ethanol to make the final volume of ethanol reach about 90%, stir and put it in a refrigerator at 4°C overnight. The obtained precipitate was collected by filtering with a 200-mesh nylon filter, and dried in a vacuum oven at 40° C. for 12 hours to obtain the water extract powder of saffron petals.

[0036] Dissolve the water extract powder of saffron petals in ultrapure water, transfer it into a D3000 dialysis bag, clam...

Embodiment 2

[0043] Accurately weigh 3 parts of air-dried saffron petal powder after alcohol extraction, each 5g, put them in 3 similar triangular flasks respectively, add 100mL of distilled water according to the ratio of material to water 1:20, stir and mix well with a glass rod . After the plastic preservative film was sealed, it was placed in a 98°C water bath for extraction for 2.8 hours. Combine the filtrates filtered through the Buchner funnel and repeat the suction filtration again, then concentrate under reduced pressure in a rotary evaporator to the minimum volume, add ethanol to make the final volume of ethanol reach 88%, stir and put it in a refrigerator at 4°C overnight. The obtained precipitate was collected by filtering with a 200-mesh nylon filter, and dried in a vacuum oven at 40° C. for 12 hours to obtain the water extract powder of saffron petals.

[0044] Dissolve the water extract powder of saffron petals in ultrapure water, transfer it into a D3000 dialysis bag, clam...

Embodiment 3

[0051] Detect the saffron petal total polysaccharide that obtains in embodiment 1 to DPPH scavenging ability

[0052] The polysaccharide was formulated into a solution with a concentration of 1, 2, 4, 8, and 16 mg / mL, and the DPPH scavenging ability was measured according to the following steps, with three parallels for each concentration, and the average value was taken.

[0053] Table 1 Experimental setup of saffron petal total polysaccharides (PPC) on DPPH scavenging ability

[0054]

[0055] Place it in the dark at room temperature for 30min, measure the absorbance at 517nm

[0056] The average value of each tube is marked A1 A2 A0

[0057] Clearance rate (%)=[1-(A1-A2) / A0]×100%

[0058] The results of the scavenging rate test of DPPH free radicals by 6 different concentrations of PPC solutions are shown in figure 1 , we can see that with the increase of the mass concentration of PPC, the scavenging effect on DPPH free radicals is gradually enhanced, which has a cert...

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Abstract

The invention provides a method for extracting saffron petal total polysaccharide, belonging to the technical field of saffron active ingredient extraction. The method comprises the following steps: the saffron petal is extracted with ethanol and air-dried and the filter residue is mixed with water at a ratio of 1 g to (15 to 25) ml and then extracted at 95-105 DEG C for 3 hours, and then alcoholprecipitation is carried out to obtain a saffron petal total polysaccharide crude product; the saffron petal total polysaccharide crude product is redissolved and subjected to chromatographic purification by chromatography column so as to obtain the saffron petal total polysaccharide. The method disclosed by the invention has high saffron petal total polysaccharide yield, high activity, high DPPHfree radical and .OH scavenging ability. The acute toxicity evaluation is practically non-toxic, which can reduce the contents of TC and TG in the liver to certain extent. The saffron petal polysaccharide is indicated to have the functions of lowering blood lipid levels, effectively preventing high blood lipid levels, and effectively preventing hyperlipidemia caused by long-term high-fat diet.

Description

technical field [0001] The invention belongs to the technical field of saffron active ingredient extraction, in particular to saffron petal total polysaccharides and an extraction method and application thereof. Background technique [0002] Saffron (Saffron, Crocus sativus L.), also known as saffron, saffron, saffron blue, and Safaji, is a perennial herb of the genus Saffron of the family Iridaceae. According to the "Chinese Pharmacopoeia", its medicine Use part is dried style, and its effect of saffron as traditional Chinese medicine is: blood circulation promoting and blood stasis dispelling, cooling blood and detoxification, resolving stagnation and calming the nerves. It is used for amenorrhea in the abdomen, postpartum stasis, febrile rash caused by warm toxin, depression and nausea, palpitation and madness, etc. In addition to medicinal purposes, it can be used as a food additive, food coloring and advanced flavoring. Pharmacological studies on saffron in recent yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/06A61K8/73A61Q19/00
CPCA61K8/73A61K31/715A61K2800/10A61P3/06A61Q19/00C08B37/0003C08B37/006
Inventor 李振宇王晓彤胡凌娟钱华李明焱
Owner JINHUA SHOUXIANGU PHAMACEUTICAL CO LTD
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