System for analyzing pulsed electron paramagnetic resonance data of complex multimeric protein receptor, method and application

Abstract

The invention discloses a system for analyzing the pulsed electron paramagnetic resonance data of a complex multimeric protein receptor, a method and application. The system comprises a module A, a module B, a module C and a module D; the module A is used for expressing a multimeric receptor protein through cell engineering and collecting and processing the distribution of spin labels in distancesbetween the subunit marker sites of the receptor protein under natural ligand stimulation; the module B is used for selecting some optimal protein structure models conforming to experimental data from the pulsed electron paramagnetic resonance map of the multimeric protein passage receptor according to the structural information of receptor protein electromagnetic resonance which is analyzed under the ligand stimulation; the module C is used for microscopically adjusting the optimal structural models through molecular manipulation techniques, comparing the distribution of spin label marker rotamers so as to find experimental data and structure and conformation information represented by part of a back portion which cannot be matched by the module B; and the module D is used for quantitatively determining the spatial structural features of the effect targets of a clinical drug in a transmembrane region under the action of ligands.

Description

technical field [0001] The invention relates to the fields of protein biotechnology, biophysics, and drug targeting mechanism, and specifically relates to a system, method and application for analyzing pulsed electron paramagnetic resonance data of complex multimeric protein receptors. Background technique [0002] Ligand-activated gated neural ion channels are located in the post-synapse of neurons, and are a large class of receptors that accept the stimulation of neurotransmitters released by pre-synaptic neurons to open their own ion channels and regulate the conduction of nerve potentials. These ion channels with different functions are responsible for the conduction and regulation of brain nerve signals, and affect human learning, memory, and emotional regulation. The structure is highly homologous, and they are all axisymmetric ion channels surrounded by five subunits. There will be a loop structure bounded by two cysteines in the extracellular region of each subunit n...

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Problems solved by technology

Based on the above reasons, for the γ-aminobutyric acid receptor (α1 / β3) reconstituted on the liposome to mimic the receptor post-synaptic cell membrane, spin labeling at the target site of the clinical anesthetic drug R-etomidate β3M286 The resulting distance distribution may be more complex than the theoretical long / short distance distribution because there is more than one conformation and the result may not be a set of distance peaks

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