A detection method for exogenous organic pollutants in plants

A technology of organic pollutants and detection methods is applied in the field of detection of exogenous organic pollutants in plants, and achieves the effects of efficient and rapid detection, shortened time, and simplified sample pretreatment steps.

Active Publication Date: 2020-12-25
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of this, the technical problem to be solved by the present invention is to provide a detection method for exogenous organic pollutants in plants. The detection method provided by the present invention can detect exogenous organic pollutants in plants well, and can observe Spatial distribution of source organic pollutants in plants

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  • A detection method for exogenous organic pollutants in plants
  • A detection method for exogenous organic pollutants in plants
  • A detection method for exogenous organic pollutants in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Substrate selection:

[0119] (1) Accurately draw 1 μL of 1-butyl-3-methylimidazole bromide standard solution with a concentration of 1mg / L with a pipette gun, spot the sample on a stainless steel target plate, and wait for it to dry naturally, then use a pipette gun to Accurately draw 1 μL of DHB matrix solution with a concentration of 10g / L, and place it on the air-dried position. Co-crystals are formed under natural drying conditions, and the target plate is put into a mass spectrometer for analysis.

[0120] Mass spectrometry method:

[0121] Positive ion reflection detection mode is applied, the mass range is 0~1000Da, and the sampling rate is 2.0GS / s;

[0122] Using 337nm nitrogen source laser, laser energy 60%, laser frequency 30.0Hz;

[0123] Number of laser clicks: 100 per map;

[0124] Reflector voltage 1800V, ion source acceleration voltage 18kV, ion source vacuum 3.0×10 -6 mbar.

[0125] Other parameters are system default values.

[0126] Thereby obt...

Embodiment 2

[0145] The setting of the blank control group:

[0146] The pea seeds were cultivated in the nutrient solution for 4 weeks, and the obtained pea plants were used as a blank control group.

[0147] 1. Pretreatment of blank samples:

[0148] A pea plant was selected, 1 cm of root tissue was cut off, and sliced ​​by a cryostat. The thickness of the obtained tissue slices was 100 μm. The tissue sections were fixed on an indium tin oxide conductive glass slide, and freeze-dried at a vacuum degree of -0.08MPa at a temperature of -60°C for 24 hours. Then, use the ImagePrep instrument (Bruker Daltonics) to deposit a DCTB matrix solution with a concentration of 10 g / L. The solvent is dichloromethane. The deposition needs to undergo 2 s of spraying, 30 s of incubation and 60 s of drying. After drying, continue to repeat the 2 s process. Spraying, incubation for 30 s and drying for 60 s, the number of repetitions was 59 times to obtain a pretreatment blank sample.

[0149] 2. Mass spe...

Embodiment 3

[0170] The setting of the blank control group:

[0171] The pea seeds were cultivated in the nutrient solution for 4 weeks, and the obtained pea plants were used as a blank control group.

[0172] 1. Pretreatment of blank samples:

[0173] A pea plant was selected, 1 cm of root tissue was cut off, and sliced ​​by a cryostat. The thickness of the obtained tissue slices was 100 μm. The tissue sections were fixed on an indium tin oxide conductive glass slide, and freeze-dried at a vacuum degree of -0.08MPa at a temperature of -70°C for 24 hours. Then, use the ImagePrep instrument (Bruker Daltonics) to deposit a DCTB matrix solution with a concentration of 10 g / L. The solvent is dichloromethane. The deposition needs to undergo 2 s of spraying, 30 s of incubation and 60 s of drying. After drying, continue to repeat the 2 s process. Spraying, incubation for 30 s and drying for 60 s, the number of repetitions was 59 times to obtain a pretreatment blank sample.

[0174] 2. Mass spe...

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Abstract

The invention provides a method for detecting exogenous organic pollutants in plants. By using a specific matrix solution, and cooperating with other steps, the exogenous organic pollutants in the plants can be well detected, and the spatial distribution condition of the exogenous organic pollutants in the plants can be observed. Compared with a traditional method for detecting the pollutants in the plants, according to the method for detecting the exogenous organic pollutants in the plants, detection is efficient and fast, and various compounds with different mass-to-charge ratios can be further analyzed simultaneously, so that establishment and optimization of an individual compound method are not required. Meanwhile, complex sample pretreatment steps in the traditional method are simplified, the time is greatly shortened, the time and labor are saved, and biochemical reactions of the plants in the pretreatment process are avoided; and meanwhile the loss of to-be-measured target molecules is reduced, and the accuracy of analysis results is improved.

Description

technical field [0001] The invention relates to the field of analysis and detection of organic pollutants, in particular to a method for detecting exogenous organic pollutants in plants. Background technique [0002] Trace amounts of organic matter are ubiquitous in environmental natural water bodies, and these micro-pollutants are refractory, bioaccumulative, and long-term harmful. Some studies have found that certain environmental micropollutants can have varying degrees of toxic effects on plants and animals. Traditional analysis methods for exogenous pollutants absorbed by plants mainly include: high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) Wait. However, before sample analysis by chromatography and chromatography-mass spectrometry, a series of complex sample pretreatment processes are required, which is not only time-consuming and laborious, but also compl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64G01N1/42
CPCG01N1/42G01N27/64
Inventor 盛国平贾宇宁尹浩
Owner UNIV OF SCI & TECH OF CHINA
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