Synthesis and modification method of upconversion nanometer material

A technology of nanomaterials and modification methods, which is applied in the fields of wave energy or particle radiation treatment materials, pharmaceutical formulations, drug combinations, etc., can solve the problems of lack of tumor tissue targeting, insignificant treatment effect, and difficulty in transmitting tumors, and achieves strong The luminous conversion efficiency, the effect is good, the effect of wide application prospect

Inactive Publication Date: 2019-01-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, traditional photodynamic therapy has the following problems, which limit its application in medicine
1) Porphyrin derivatives are commonly used photosensitizers at this stage. They lack targeting to tumor tissue, and it is difficult to reach a certain concentration in the tumor, and the therapeutic effect is not obvious
2) Most of the photosensitizer molecules are hydrophobic molecules, which are easy to aggregate in the body and difficult to transport to the tumor

Method used

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  • Synthesis and modification method of upconversion nanometer material
  • Synthesis and modification method of upconversion nanometer material
  • Synthesis and modification method of upconversion nanometer material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Add 3 mL of oleic acid and 15 mL of 1-octadecene into a round bottom flask, and stir. The mixture was heated to 70°C under nitrogen atmosphere. Mix 397 μL YCl 3 , 300 μL YbCl 3, 30 μL ErCl 3 , 300 μL GdCl 3 Preparation of NaYF 4 : Yb / Er / Gd (30,0.3,30mol%) solution. The solution was added into the reaction liquid, and heated to 150° C., and kept warm for 30 minutes to obtain a clear and uniform solution.

[0049] (2) Cool the reaction solution in step (1) to 65°C, and add 10mL of 0.1g NaOH and 0.148gNH 4 F in methanol solution. The temperature of the reaction solution was raised to 110 ° C for 20 min. Connect the condenser tube and the piston, seal the three-necked bottle, and vacuumize it for 10 minutes. Repeat the cross evacuation and nitrogen filling three times, each lasting one minute.

[0050] (3) Fill the closed space with nitrogen, raise the temperature to 300°C at a rate of 10°C / min, and maintain this temperature for 1.5h. After the reaction, it w...

Embodiment 2

[0076] Step (1) to step (5) are the same as embodiment 1.

[0077] (6) Add 21mL cyclohexane, 1.5mL IGEPAL CO-520, and 2mL UCNPs solution into a 50ml single-necked bottle, add 211μL ammonia water with a mass fraction of 25% dropwise, stir for 30min, then add 100μL TEOS dropwise, and stir for 36 hours , The stirring speed was kept at 750r / min during the experimental reaction.

[0078] (7) Pack the reaction solution in step (6) into two centrifuge tubes respectively. Rinse the three-neck flask with 20mL of absolute ethanol, and transfer to two centrifuge tubes respectively. After vortexing and centrifuging for 1 min, add 10 mL of acetone each, and vortex for 30 s. Centrifuge at room temperature with a centrifugal force of 5200g for 20min, then remove the supernatant. Add 10 mL of ethanol to each centrifuge tube, vortex and sonicate for 10 min. Pour the solutions in the two centrifuge tubes into one centrifuge tube and add 15 mL of acetone. Centrifuge at 5200 g for 20 min at ...

Embodiment 3

[0082](1) Add 3 mL of oleic acid and 15 mL of 1-octadecene into a round bottom flask, and stir. The mixture was heated to 70°C under nitrogen atmosphere. Mix 390 μL YCl3, 250 μL YbCl3, 100 μL ErCl3, 350 μL GdCl3 to prepare NaYF4:Yb / Er / Gd (25, 1, 35 mol%) solution. The solution was added to the reaction solution, and the mixture was heated to 150° C., and after 30 min of heat preservation, a clear and uniform solution was obtained.

[0083] (2) Cool the reaction solution in step (1) to 65°C, and add 10mL of 0.1g NaOH and 0.148gNH 4 F in methanol solution. The temperature of the reaction solution was raised to 110 ° C for 20 min. Connect the condenser tube and the piston, seal the three-necked bottle, and vacuumize it for 10 minutes. Repeat the cross evacuation and nitrogen filling three times, each lasting one minute.

[0084] (3) Fill the closed space with nitrogen, raise the temperature to 300°C at a rate of 10°C / min, and maintain this temperature for 1.5h. After the re...

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Abstract

The invention discloses a synthesis and modification method of an upconversion nanometer material. The upconversion nanometer material with uniform particle diameter is prepared by a thermolysis method; secondly, mesoporous silica is wrapped outside nanoparticles; surface modification is performed; core-shell nanoparticles are formed; a hydrophylic structure is constructed; finally, photosensitizers and folic acid are carried on porous nanoparticles, so that the functions of photodynamic killing and in vitro targeting on tumor cells are realized. Near-infrared light with high penetrating capability is used for excitation, so that cancer cells generate a large number of active oxygen so as to achieve the effect of inhibiting the cancer; the goal of photodynamic treatment and killing on thetumor cells is realized; the damage to cells and biological tissues is reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a method for synthesizing and modifying up-conversion nanometer materials. Background technique [0002] Photodynamic therapy (PDT) is an effective method for the treatment of cancer and tumors in modern medicine, which has the advantages of high targeting, non-toxicity and minimal invasiveness. At the same time, the purpose of increasing or changing the degree of control can be achieved by modifying parameters, such as wavelength, intensity and exposure time, which can effectively solve the safety and specificity problems in the treatment. The killing mechanism of PDT on tumors is relatively complex, and factors such as the type of photosensitizer, tissue biological characteristics, and the state of combination of photosensitizer and tumor will affect its killing effect. Due to the characteristics of high absorption and low metabolism of tumor tissue, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/54A61K41/00A61K49/00A61P35/00
CPCA61K41/0057A61K47/545A61K47/6929A61K49/0052A61K49/0093A61P35/00
Inventor 毛传斌岳慧刘项宇
Owner ZHEJIANG UNIV
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