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Method for separating or analyzing phenolic acid compound from burdock

A technology for phenolic acids and compounds, applied in the field of active ingredient separation, can solve the problems of unstable and time-consuming derivatization of phenolic acid substances, and achieve the effects of improving duty cycle and sensitivity, large peak capacity, and stable derivatization.

Inactive Publication Date: 2018-12-21
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many methods for separating and analyzing phenolic acid compounds in the prior art, but they usually have obvious disadvantages, such as taking too long, unstable derivation process of phenolic acid substances, etc.

Method used

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  • Method for separating or analyzing phenolic acid compound from burdock
  • Method for separating or analyzing phenolic acid compound from burdock
  • Method for separating or analyzing phenolic acid compound from burdock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] (1) Preparation of the test solution:

[0082] The burdock leaves were sliced ​​and dried into dry powder, then refluxed with 70% ethanol to extract for 3 hours, then added 0.15% of the total volume of hydrochloric acid to extract twice, mixed and concentrated under reduced pressure at 40°C, and then frozen Dry, add volume concentration and be mixed with the chromatographic methanol of 80% need testing solution;

[0083] (2) Prepare standard stock solution by reference product:

[0084] Weigh 3,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, trans-p-coumaric acid, quimaric acid, vanillic acid, caffeic acid, trans-ferulic acid, sinapinic acid, all with volume concentration 80% chromatographic methanol is prepared as a standard stock solution with a substance concentration of 100ppm;

[0085] (3) Preparation of mixed standard solution:

[0086] Accurately measure the standard stock solution of 8 kinds of phenolic acid compounds, use described 80% chromatographic me...

Embodiment 2

[0090] Adopt the operation process as embodiment 1, wherein:

[0091] The chromatographic conditions of high performance liquid chromatography are as follows:

[0092] The size is 2.1mm×100mm, 3.0μm C18 column, the mobile phase is composed of mobile phase A and mobile phase B, wherein mobile phase A is an aqueous solution containing 0.1% formic acid, mobile phase B is methanol or acetonitrile, and the gradient is carried out under the following conditions Elution: 0~0.05min, 5%~35% mobile phase B; 0.05~15min, 35%~55% mobile phase B; 15~25min, 55%~100% mobile phase B; volume flow rate is 0.15mL. min -1 ; The injection volume is 5 μL; min -1 ; Ion mobility spectrometry conditions are as follows:

[0093]

[0094]

[0095] The signal-to-noise ratio of the obtained ion mobility spectrum was analyzed to obtain the following figure 1 In the statistical results shown, in each group of columns in the figure, the column on the left is acetonitrile, and the column on the righ...

Embodiment 3

[0097] Use the operation process as embodiment 1, wherein:

[0098] The chromatographic conditions of the high-performance liquid chromatography are as follows: the specification is 2.1mm×100mm, 3.0μm C18 column, the mobile phase is composed of mobile phase A and mobile phase B, wherein mobile phase A is 0.1% formic acid aqueous solution or 0.1% acetic acid aqueous solution , the mobile phase B is methanol, and the following conditions are used for gradient elution: 0-0.05min, 5%-35% mobile phase B; 0.05-15min, 35%-55% mobile phase B; 15-25min, 55%-100 % mobile phase B; the volume flow rate is 0.15mL. min -1 ; The injection volume is 5 μL; the column temperature is 35°C; post-column replenishment is preferred, and the flow rate of the post-column replenishment solution is 0.1mL. min -1 ;

[0099] The ion mobility spectrometry conditions are as follows:

[0100]

[0101]

[0102] The signal-to-noise ratio of the obtained ion mobility spectrum was analyzed to obtain t...

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Abstract

The invention discloses a method for separating or analyzing a phenolic acid compound from a burdock. The method comprises a joint use process of an efficient liquid chromatograph and an ion mobilityspectrometer, wherein the ion mobility spectrometer is an electrospray-ion mobility spectrometer. Through the method, phenolic acid compound ingredients in the burdock can be quickly separated or analyzed, and an application basis is provided for new burdock medicine development.

Description

technical field [0001] The invention relates to the technical field of separating active components from burdock. Background technique [0002] Burdock A.Lappa L. is a perennial herb of Compositae, a long-sun plant, although it grows in warm climate conditions, it also has the characteristics of cold and heat resistance. Its general flowering period is June-August, and fruit period is August-October. The planting areas in my country include Shandong, Jiangsu, Taiwan, Shaanxi, Henan, Hubei, Anhui, Zhejiang and other places. Not only can it be eaten as a high-grade vegetable for physical fitness, but it can also be used as medicine, wine making, and health care products. [0003] Burdock contains a variety of active ingredients, such as volatile oil, polyphenols, polyacetylenes, thioacetylenes, polysaccharides, etc. The phenolic acids contained in burdock have attracted more and more attention nowadays, and studies have proved that they have physiological activities such as...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/02G01N30/72
CPCG01N30/02G01N30/7266G01N30/88G01N2030/027G01N2030/884
Inventor 刘圆杨正明刘春林李莹黄艳菲天喜格勒
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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